Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Genome-wide exploration of metal tolerance protein (MTP) genes in common wheat (Triticum aestivum): insights into metal homeostasis and biofortification
Date
2017-04-01
Author
Vatansever, Recep
Filiz, Ertugrul
Eroğlu, Seçkin
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
175
views
0
downloads
Cite This
Metal transport process in plants is a determinant of quality and quantity of the harvest. Although it is among the most important of staple crops, knowledge about genes that encode for membrane-bound metal transporters is scarce in wheat. Metal tolerance proteins (MTPs) are involved in trace metal homeostasis at the sub-cellular level, usually by providing metal efflux out of the cytosol. Here, by using various bioinformatics approaches, genes that encode for MTPs in the hexaploid wheat genome (Triticum aestivum, abbreviated as Ta) were identified and characterized. Based on the comparison with known rice MTPs, the wheat genome contained 20 MTP sequences; named as TaMTP1-8A, B and D. All TaMTPs contained a cation diffusion facilitator (CDF) family domain and most members harbored a zinc transporter dimerization domain. Based on motif, phylogeny and alignment analysis, A, B and D genomes of TaMTP3-7 sequences demonstrated higher homology compared to TaMTP1, 2 and 8. With reference to their rice orthologs, TaMTP1s and TaMTP8s belonged to Zn-CDFs, TaMTP2s to Fe/Zn-CDFs and TaMTP3-7s to Mn-CDFs. Upstream regions of TaMTP genes included diverse cis-regulatory motifs, indicating regulation by developmental stage, tissue type and stresses. A scan of the coding sequences of 20 TaMTPs against published miRNAs predicted a total of 14 potential miRNAs, mainly targeting the members of most diverged groups. Expression analysis showed that several TaMTPs were temporally and spatially regulated during the developmental time-course. In grains, MTPs were preferentially expressed in the aleurone layer, which is known as a reservoir for high concentrations of iron and zinc. The work identified and characterized metal tolerance proteins in common wheat and revealed a potential involvement of MTPs in providing a sink for trace element storage in wheat grains.
Subject Keywords
CDF
,
Micronutrient
,
Aleurone
,
Deficiency
,
Biofortification
URI
https://hdl.handle.net/11511/40742
Journal
BIOMETALS
DOI
https://doi.org/10.1007/s10534-017-9997-x
Collections
Department of Biology, Article
Suggestions
OpenMETU
Core
Kinetic analysis of aspergillus oryzae cultivations on starch
Bayındırlı, Alev; UNGAN, S (Informa UK Limited, 1991-01-01)
Cultivation of Aspergillus oryzae on starch is described as a combination of two rate processes: Starch hydrolysis and the cellular activities of the fungi including growth, enzyme production and maintenance. Kinetic models are presented to describe growth, enzyme production, starch hydrolysis and uptake of the hydrolysis products. Numerical values of the model parameters indicated that the rate controlling step of A. oryzae growth on starch was not starch hydrolysis, but the substrate uptake process. Gluco...
Modeling and simulation of photobioreactors for biological hydrogen production
Androga, Dominic Deo; Eroğlu, İnci; Uyar, Başar; Department of Biotechnology (2014)
In applications of photofermentative hydrogen production, maintaining optimal temperature, feed composition, pH range and light intensity is the most critical objective for growth and proper functioning of the photosynthetic bacteria. Response Surface Methodology was applied to optimize temperature and light intensity for indoor hydrogen production using Rhodobacter capsulatus. Surface and contour plots of the regressions models developed revealed a maximum hydrogen production rate of 0.566 mol H2/m3/h at 2...
Development of Random Amplified Polymorphic DNA (RAPD) markers in 150 F-6-inbred durum wheat (Triticum durum) lines derived from Kunduru-1149 x Cham-1 cross
Göçmen, Birşen; Kaya, Zeki (2003-01-01)
Random Amplified Polymorphic DNA (RAPD) markers were developed in a specific durum wheat population (150 lines) derived from Kunduru-1149 x Chain-1 cross. Of 284 RAPD primers screened against parent DNAs, 83 revealed at least one polymorphic locus. The most polymorphic and clear bands producing 13 primers were selected and screened with the DNAs of 150 lines. These 13 primers revealed a total number of 33 segregating loci. The preliminary results of this study could be used as a base for mapping economicall...
Theory of impurity induced lattice distortions in the normal phase of charge density wave systems
Turgut, Sadi (1994-05-01)
A phenomenological Ginzburg-Laundau theory is applied to the normal phase of one-dimensional charge-density-wave systems with a finite concentration of impurities. It is found that the interaction between the impurities and the highly polarizable electron gas leads to a strong and oscillatory impurityimpurity interaction, which in turn leads to ordered impurity arrangements and to sizeable periodic lattice distortions. The effect is very strongly dependent on the charge of the impurities, their concentratio...
Genome-wide analysis of gene expression profiling revealed that COP9 signalosome is essential for correct expression of Fe homeostasis genes in Arabidopsis
Eroğlu, Seçkin (Springer Science and Business Media LLC, 2017-10-01)
In plant cells, either excess or insufficient iron (Fe) concentration triggers stress responses, therefore it is strictly controlled. Proteasome-mediated degradation through ubiquitination of Fe homeostasis proteins has just become the focus of research in recent years. Deactivating ubiquitin ligases, COP9 signalosome has a central importance in the translational control of various stress responses. The aim of the study was to investigate COP9 signalosome in Fe deficiency response of Strategy I plants. In s...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
R. Vatansever, E. Filiz, and S. Eroğlu, “Genome-wide exploration of metal tolerance protein (MTP) genes in common wheat (Triticum aestivum): insights into metal homeostasis and biofortification,”
BIOMETALS
, pp. 217–235, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40742.