Assessment and optimization of xylanase production using co-cultures of Bacillus subtilis and Kluyveromyces marxianus

Yardimci, Gozde Oren
Çekmecelioğlu, Deniz
Co-cultures of Bacillus subtilis and Kluyveromyces marxianus were investigated in submerged fermentation for xylanase production at shake-flask scale. Xylanase production markedly increased when arabinose, xylose, or hazelnut shells were used as the single carbon source. Maximal xylanase of 49.5 IU/mL was achieved with 4% B. subtilis, 4% K. marxianus, 40% solid load of hazelnut shells, and pH 7.0. Overall, xylanase was enhanced by 4.4-fold compared to initial un-optimized monoculture production and 2.8-fold compared to initial un-optimized co-cultured production, after optimization by response surface method.


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Optimization of the solid-liquid extraction conditions for trans-resveratrol, trans-E-viniferin, ferulic acid, and total phenolics from milled grape canes has been investigated. The temperature and ethanol concentration were found to be major process variables for all responses, whereas the solvent to solid ratio was found not to be significant for any of the responses studied. The yields of transresveratrol, trans-epsilon-viniferin, and total phenolics increased with increasing temperature. Maximum yields ...
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Citation Formats
G. O. Yardimci and D. Çekmecelioğlu, “Assessment and optimization of xylanase production using co-cultures of Bacillus subtilis and Kluyveromyces marxianus,” 3 BIOTECH, pp. 0–0, 2018, Accessed: 00, 2020. [Online]. Available: