Evaluation of sericin/collagen membranes as prospective wound dressing biomaterial

Date

2011-09-01

Author

AKTÜRK, Omer
Tezcaner, Ayşen
BİLGİLİ, Hasan
Deveci, M. Salih
Gecit, M. Rusen
Keskin, Dilek

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

185
views

0
downloads

Sericin, a silk protein, has high potential for use in biomedical applications. In this study, wound dressing membranes of Sericin (S) and Collagen (C) were prepared by glutaraldehyde cross-linking at S/C; 2:1,1:1, 1:2, and 0:1 weight ratios. They were stable in water for 4 weeks. However, increasing the proportion of sericin had decreasing effect on the membrane stability. Water swelling property of membranes was enhanced with sericin. The highest water swelling was obtained in 1:1 group (9.06 g/g), but increasing collagen or sericin content in the membranes had a diminishing effect. Highest water vapor transmission rate was obtained with 1:2 group (1013.80 g/m(2)/day). Oxygen permeability results showed that 1:2 (7.67 mg/L) and 2:1 (7.85 mg/L) SIC groups were better than the other groups. While sericin decreased the tensile strength and elongation of membranes, it increased modulus. Sericin also increased brittleness of membranes, but their UTS range (24.93-44.92 MPa) was still suitable for a wound dressing. Membranes were not penetrable to microorganisms. Cytotoxicity studies showed that fibroblasts and keratinocytes attached and gained their characteristic morphologies. They also proliferated on membranes significantly. After 1 week of subcutaneous implantation, a fibrous capsule formed around all membranes with an acute inflammation. Sericin containing membranes showed signs of degradation (at 2nd week), while collagen only membranes remained largely intact. Eventually, sericin containing membranes degraded in 3 weeks with moderate inflammatory response. Overall results suggest that sericin/collagen membranes would be favorable as wound dressing material when sericin ratio is less than or equal to the collagen component. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.

Subject Keywords

Sericin, Collagen, Wound dressing, Membrane, Biocompatibility

URI

https://hdl.handle.net/11511/35420

Journal

JOURNAL OF BIOSCIENCE AND BIOENGINEERING

DOI

https://doi.org/10.1016/j.jbiosc.2011.05.014

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

Evaluation of Staphylococcus aureus DNA aptamer by enzyme-linked aptamer assay and isothermal titration calorimetry BAYRAÇ, CEREN; Öktem, Hüseyin Avni (2017-02-01) To monitor the specificity of Staphylococcus aureus aptamer (SA-31) against its target cell, we used enzyme-linked aptamer assay. In the presence of target cell, horseradish peroxidase-conjugated streptavidin bound to biotin-labeled SA-31 showed specific binding to S aureus among 3 different bacteria with limit of detection of 103 colony-forming unit per milliliter. The apparent K-a was 1.39 mu M-1 +/- 0.3 mu M-1. The binding of SA-31 to membrane proteins extracted from cell surface was characterized using ...
Life cycle assessment of sericin recovery from silk degumming wastewaters ÇAPAR, GÖKŞEN; PİLEVNELİ, TOLGA; Yetiş, Ülkü; Dilek, Filiz Bengü (2022-12-01) Sericin is a silk protein discarded with silk degumming wastewater. Life cycle assessment of sericin recovery from silk degumming wastewater was conducted considering two scenarios; base scenario (no recovery), and sericin recovery with membrane hybrid processes. Base scenario consists of silk degumming and treatment of degumming wastewater. The recovery scenario consists of sericin recovery with the process train; silk degumming, sedimentation, nanofiltration (NF), precipitation and lyophilization. Spray d...
Development and characterization of composite proton exchange membranes for fuel cell applications Akay, Ramiz Gültekin; Baç, Nurcan; Department of Chemical Engineering (2008) Intensive research on development of alternative low cost, high temperature membranes for proton exchange membrane (PEM) fuel cells is going on because of the well-known limitations of industry standard perfluoro-sulfonic acid (PFSA) membranes. To overcome these limitations such as the decrease in performance at high temperatures (>80 0C) and high cost, non-fluorinated aromatic hydrocarbon based polymers are attractive. The objective of this study is to develop alternative membranes that possess comparable ...
Synthesis and characterization of copolymers of diisocyanates and dialcohol Keskin, Selda; Usanmaz, Ali; Department of Polymer Science and Technology (2008) This study was aimed to synthesize low molecular weight hydroxyl terminated polyurethane acrylate polymers that can be used in biomedical applications. Acrylate end capping via inter-esterification reaction was successfully achieved with the methacryloyl chloride addition to the hydroxyl ends of the polyurethane at low temperatures. Isocyanate terminated polyurethane acrylates were also synthesized for the sake of comparison. TDI, HDI and MDI were used as diisocyanates for urethane synthesis and they were e...
Analysis of protein-protein interaction networks using random walks Can, Tolga; Singh, Ambuj K. (2005-08-21) Genome wide protein networks have become reality in recent years due to high throughput methods for detecting protein interactions. Recent studies show that a networked representation of proteins provides a more accurate model of biological systems and processes compared to conventional pair-wise analyses. Complementary to the availability of protein networks, various graph analysis techniques have been proposed to mine these networks for pathway discovery, function assignment, and prediction of complex mem...

Citation Formats

O. AKTÜRK, A. Tezcaner, H. BİLGİLİ, M. S. Deveci, M. R. Gecit, and D. Keskin, “Evaluation of sericin/collagen membranes as prospective wound dressing biomaterial,” JOURNAL OF BIOSCIENCE AND BIOENGINEERING, pp. 279–288, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35420.