Immunogenicity and protective efficacy of the recombinant Pasteurella lipoprotein E and outer membrane protein H from Pasteurella multocida A:3 in mice

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2012-12-01

Author

Okay, Sezer
Ozcengiz, Erkan
GÜRSEL, İHSAN
Özcengiz, Gülay

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Pasteurella multocida serotype A:3 is a Gram-negative bacterial pathogen, one of the causative agents of shipping fever of cattle. In this study, outer membrane protein H (ompH) and Pasteurella lipoprotein E (plpE) genes were cloned and plpEC-ompH fusion was constructed and expressed in Escherichia coli. Recombinant PlpE, OmpH and PlpEC-OmpH fusion proteins were purified and formulated with oil-based and oil-based CpG ODN adjuvants. Antibody responses in mice vaccinated with recombinant PlpE and PlpEC-OmpH proteins formulated with both adjuvants were significantly (p < 0.05) increased. However, a significant (p < 0.05) increment in serum IFN-gamma level was only observed upon immunization with oil-based CpG formulations. Protectivity of the vaccines were evaluated via intraperitoneal challenge of mice with 10 LD50 of P. multocida A:3. The recombinant proteins PlpE and PlpEC-OmpH fusion conferred 100% protection when formulated with oil-based CpG ODN while the protectivity was found to be 80% and 60%, respectively when only oil-based adjuvant was used in respective formulations. These findings indicated that the recombinant PIPE or PlpEC-OmpH fusion proteins formulated with oil-based CpG ODN adjuvant are possible acellular vaccine candidates against shipping fever.

Subject Keywords

Outer membrane protein H, Pasteurella lipoprotein E, Pasteurella multocida, Recombinant vaccine

URI

https://hdl.handle.net/11511/35723

Journal

RESEARCH IN VETERINARY SCIENCE

DOI

https://doi.org/10.1016/j.rvsc.2012.05.011

Collections

Department of Biology, Article

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Citation Formats

S. Okay, E. Ozcengiz, İ. GÜRSEL, and G. Özcengiz, “Immunogenicity and protective efficacy of the recombinant Pasteurella lipoprotein E and outer membrane protein H from Pasteurella multocida A:3 in mice,” RESEARCH IN VETERINARY SCIENCE, pp. 1261–1265, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35723.