Selective recognition of anionic cell membranes using targeted liposomes coated with zinc(II)-bis(dipicolylamine) affinity units

2014-08-14
Türkyılmaz, Serhan
Palumbo, Rachael
Smith, Bradley D.
Zinc(II)-bis(dipicolylamine) (Zn(2)BDPA) coated liposomes are shown to have high recognition selectivity towards vesicle and cell membranes with anionic surfaces. Robust synthetic methods were developed to produce Zn(2)BDPA-PEG-lipid conjugates with varying PEG linker chain length. One conjugate (Zn(2)BDPA-PEG(2000)-DSPE) was used in liposome formulations doped with the Lipophilic near-infrared fluorophore DiR. Fluorescence cell microscopy studies demonstrated that the multivalent Liposomes selectively and efficiently target bacteria in the presence of healthy mammalian cells and cause bacterial cell agglutination. The Liposomes also exhibited selective staining of the surfaces of dead or dying human cancer cells that had been treated with a chemotherapeutic agent.
ORGANIC & BIOMOLECULAR CHEMISTRY

Suggestions

Insertion of Fischer Carbene Complexes into the Carbon-Carbon Bond of 1,2-Diphenylcyclopropenone: Formation of Cyclobutenones and o- and p-Methoxyphenol Derivatives
Zora, Metin (American Chemical Society (ACS), 1994-08-01)
Thereactionbetween1,2-diphenylcyclopro-penoneandFischercarbenecomplexeshasbeeninves-tigated.Thereactionproducedamixtureofdipheny-lacetyleneandcyclobutenones.Whena,ß-unsaturatedcarbenecomplexeswereemployed,benzannulationprod-uctsanddiphenylacetylenewereproduced.Amecha-nisminvolvingmetallacyclobutenoneformation,followedbyeithercarbeneinsertionandreductiveeliminationorfragmentationhasbeenproposed.Recently,wereportedthatcarbon—carbonbondin-sertionisthemajorreactionpathwaywhenFischercarbenecomplexesreactwith1,2-...
Synthesis, characterization, and electrochemistry of tetracarbonyl(6-ferrocenyl-2,2 '-bipyridine)tungsten(0)
Edinc, Pelin; Oenal, Ahmet M.; Özkar, Saim (Elsevier BV, 2007-04-15)
6-Ferrocenyl-2,2'-bipyridine (fcbpy) was prepared by the reaction of lithiated ferrocene with bipyridine and employed as a bidentate ligand for the formation of tetracarbonyl(6-ferrocenyl-2,2'-bipyridine)tungsten(0). The labile complex pentacarbonyl[eta(2)-bis(trimethylsilyl)ethyne]tungsten(0) reacts with fcbpy in CH2Cl2 to yield the disubstitution product, W(CO)(4)(fcbpy), which was isolated as analytically pure substance and characterized by elemental analysis, IR, UV-Vis, MS, H-1 and C-13 NMR spectroscop...
Dipole moments of some styrene copolymers
Küçükyavuz, Zühal; Baysal, Bahattin (American Chemical Society (ACS), 1988-7)
Dipolemomentsofpoly(styrene-co-p-chlorostyrene),poly(styrene-co-p-methoxystyrene),poly(styrene-co-4-vinylpyridine),andpoly(styrene-co-N-vinylcarbazole)copolymersweredeterminedintoluenesolutionsatdifferenttemperatures.Thesamplesweresynthesizedbyusingfree-radicalinitiators.Theeffectsofthenatureofthepolargroup,composition,andtemperatureonthedipolemomentratiowereinvestigated.Themean-squaredipolemomentperstructuralunitshowsapositivedeviationfromlinearity,inagreementwithcalculationsbyMarkbasedonrotationalisomeric...
Ruthenium(III) acetylacetonate: A homogeneous catalyst in the hydrolysis of sodium borohydride
Keceli, Ezgi; Özkar, Saim (Elsevier BV, 2008-05-01)
Ruthenium(ill) acetylacetonate was employed for the first time as homogeneous catalyst in the hydrolysis of sodium borohydride. Ruthenium(III) acetylacetonate was not reduced by sodium borohydride under the experimental conditions and remains unchanged after the catalysis. Poisoning experiments with mercury and trimethylphosphite provide compelling evidence for the fact that ruthenium(III) acetylacetonate is indeed a homogenous catalyst in the hydrolysis of sodium borohydride. Kinetics of the ruthenium(III)...
Crosslinking of Trimethylene Carbonate and D,L-Lactide (Co-) Polymers by Gamma Irradiation in the Presence of Pentaerythritol Triacrylate
Bat, Erhan; Feijen, Jan; Grijpma, Dirk W. (Wiley, 2011-07-07)
High-molecular-weight (co) polymers of trimethylene carbonate and D, L-lactide are efficiently crosslinked using PETA during gamma irradiation. Form-stable networks with gel contents of 86 +/- 5 to 96 +/- 1 are obtained from non-crystalline (co) polymers. Glass transition temperatures and elastic moduli of the networks can be varied by adjusting the copolymer composition. The PETA-containing (co) polymer networks are not cytotoxic. Upon incubation in macrophage cultures for 14 d, all (co) polymer films and ...
Citation Formats
S. Türkyılmaz, R. Palumbo, and B. D. Smith, “Selective recognition of anionic cell membranes using targeted liposomes coated with zinc(II)-bis(dipicolylamine) affinity units,” ORGANIC & BIOMOLECULAR CHEMISTRY, pp. 5645–5655, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35815.