Simple and Rapid Method for the Simultaneous Determination of Cholesterol and Retinol in Meat Using Normal-Phase HPLC Technique

Date

2018-02-01

Author

DOMINGUEZ, Ruben
BARBA, Francisco J.
CENTENO, Juan A.
PUTNIK, Predrag
Alpas, Hami
LORENZO, Jose M.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

125
views

0
downloads

A direct saponification and normal-phase high-performance liquid chromatography (NP-HPLC) procedure was developed for simultaneous determination of cholesterol and retinol in meat. A normal-phase silica column fitted with diode array detection at 208 nm for cholesterol and fluorescence detection (lambda-excitation 344 nm/lambda-emission 472 nm) for retinol, with mobile phase consisting of 2% (v/v) 2-propanol in n-hexane was used. Cholesterol was eluted at 10 min and retinol at 12.66 min. High linearity (R (2) > 0.9996 for both compounds) in calibration range was obtained. The LOD and LOQ values show the high sensitivity of the developed methodology for simultaneous determination of cholesterol and retinol in meat. Recovery results obtained in this study (98.67-102.14% for cholesterol and 91.72-98.27% for retinol) were between AOAC recommendations to validated method and were comparable to most recent studies in precision and accuracy. In addition, the present method showed high repeatability and reproducibility. As a general conclusion, the results indicate that the direct saponification, extraction, and HPLC analysis is an adequate method for cholesterol and retinol analysis in meat samples.

Subject Keywords

Food analysis, Cholesterol, Vitamin A, Meat, Method validation, HPLC-PAD-FL

URI

https://hdl.handle.net/11511/36170

Journal

FOOD ANALYTICAL METHODS

DOI

https://doi.org/10.1007/s12161-017-1001-4

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Mathematical modeling of swelling in high moisture whey protein gels Öztop, Halil Mecit (Elsevier BV, 2011-09-01) Gels prepared from whey proteins can be used for controlled release of nutrients or active ingredients in food systems. The objective of this study was to characterize the water uptake by these hydrophilic gels to aid in the design of release systems. Whey protein isolate (WPI) gels (17% w/w protein) of different aspect ratios were submersed in aqueous solution at pH 7.0. Modeling of mass uptake is presented in terms of Case I (Fickian diffusion) and Case II (kinetic) models. Due to the extent of swelling, ...
Selenium speciation in chicken breast samples from inorganic and organic selenium fed chickens using high performance liquid chromatography-inductively coupled plasma-mass spectrometry BAKIRDERE, Sezgin; Volkan, Mürvet; Ataman, Osman Yavuz (2018-08-01) Speciation of selenium in chicken breast samples was performed by anion and cation exchange HPLC systems combined with ICP-MS. Samples were categorized into three groups consisting of Control Group, Inorganic Se Fed Group and Organic Se Fed Group. After the optimization of speciation parameters, selenomethionine, selenocystine, Se(IV) and Se(VI) were determined in the samples using the HPLC-ICP-MS system. Instrumental limit of detection values for selenium(IV), selenium(VI), Se in selenomethionine and Se in...
Some of the frequently used biochemical values of serum and plasma in three different populations of Anatolian shepherd dog Altunok, V; Maden, M; Nizamlioglu, M; Togan, İnci Zehra (2001-03-01) The biochemical values serum albumin, total cholesterol, magnesium (Mg), calcium (Ca), inorganic phosphorus (Pi), sodium (Na). potassium (K) in serum (nl = 38) and zinc (Zn) and copper (Cu) in plasma (n2 = 74) were determined in individuals of Anatolian shepherd dog (Asd) which is the most preferred dog in Anatolia as a guard dog of flocks. Individuals from both sexes were collected from three different farms (sites). Comparative studies showed that (i) there were no significant differences between the valu...
Current applications of exopolysaccharides from lactic acid bacteria in the development of food active edible packaging Moradi, Mehran; Guimarães, Jonas T; Şahin, Serpil (Elsevier BV, 2021-08-01) The lactic acid bacteria (LAB) are usually recognized as safe for consumption and comprise several genera with different technological and health-promoting potential for food applications, including probiotic characteristics. Their exopolysaccharides (EPS) have interesting film-forming properties and may be used to produce edible packaging, as a structural enhancer, a bioactive agent or probiotic carrier in edible films and coatings. In these days, there is a specific demand for food products with reduced a...
Preparation of cross-linkedtyrosinase aggregates Aytar, Burcu Selin; Bakır, Ufuk; Department of Chemical Engineering (2006) The aim of this study was to prepare cross-linked enzyme aggregate (CLEA) from crude mushroom (Agaricus bisporus) extract. However, the optimization of CLEA production was performed by using pure tyrosinase. Important parameters were determined as protein, ammonium sulfate and glutaraldehyde concentrations, CLEA particle size, and cross-linking temperature and period. On the other hand, the order of ammonium sulfate and glutaraldehyde addition did not affect the yield of CLEA. Optimum CLEA preparation condi...

Citation Formats

R. DOMINGUEZ, F. J. BARBA, J. A. CENTENO, P. PUTNIK, H. Alpas, and J. M. LORENZO, “Simple and Rapid Method for the Simultaneous Determination of Cholesterol and Retinol in Meat Using Normal-Phase HPLC Technique,” FOOD ANALYTICAL METHODS, pp. 319–326, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36170.