Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L.) seedlings

Akcay, U. Celikkol
Ercan, O.
Kavas, M.
Yildiz, L.
Yılmaz, Çetin
Öktem, Hüseyin Avni
Two cultivars of peanut (Arachis hypogaea L.) which were designated as resistant (Florispan) and sensitive (Gazipasa) according to their growth retardation under drought stress conditions were compared for their oxidative damage and antioxidant responses. Sixteen days-old peanut seedlings were subjected to PEG-6000 solutions of two different osmotic potentials; -0.4 and -0.8 MPa, and various growth parameters, photosystem II activity, changes in malondialdehyde (MDA), hydrogen peroxide (H2O2) and proline levels, activities of ascorbate peroxidase (APX), catalase (CAT), peroxidase (POX) and gluthatione reductase (GR) enzymes were determined. Both cultivars exhibited water deficit at -0.8 MPa osmotic potential of PEG-6000 and H2O2 levels significantly increased during exposure to -0.4 MPa osmotic potential. However, H2O2 levels were under control in both cultivars at exposure to -0.8 MPa osmotic potential. Significant proline accumulation was observed in the tissues of cv. Florispan at -0.8 MPa osmotic potential, whereas proline accumulation did not appear to be an essential part of the protection mechanism against drought in cv. Gazipasa. No significant variation in chlorophyll fluorescence values were detected in neither of the cultivars. Enzyme activity measurements revealed that Gazipasa copes well with lesser magnitudes of drought stress by increasing the activity of mainly APX, and during harsh stress conditions, only APX maintains its activity in the tissues. In cultivar Florispan, GR activity appears to take role in lesser magnitudes of drought stress, whereas CAT and APX activities appear to be very crucial antioxidative defenses during intense stress conditions. The results indicate that, the level of proline and activities of the enzymes CAT and APX are important mechanisms for the maintenance of drought tolerance in peanut plants.


KAVAS, MUSA; Akca, Oya Ercan; Akcay, Ufuk Celikkol; Peksel, Begum; Eroğlu, Seçkin; Öktem, Hüseyin Avni; Yucel, Meral (2015-01-01)
In this study, the effects of long-term NaCl treatment were investigated in two cultivars of peanut designated as drought-resistant and drought-sensitive. Growth parameters, changes in the concentrations of MDA, H2O2 and proline, and the activities of antioxidant enzymes were determined under salinity stress. Growth parameters indicated the superiority of cv. Florispan to cv. Gazipasa under milder salinity stress treatment. However, comparative analysis of the two cultivars showed that MDA, H2O2, ion leakag...
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Baloglu, Mehmet Cengiz; Kavas, Musa; Aydin, Gulsum; Öktem, Hüseyin Avni; Yücel, Ayşe Meral (2012-01-01)
Drought stress is one of the most important yield-reducing factors in crop production. Sunflower, an oilseed crop, is severely affected by abiotic stress. In this study, 2 sunflower cultivars (Musala and Aydin) were evaluated in terms of various biochemical and physiological responses under 2 different polyethylene glycol-mediated drought stress conditions. Stress-determining parameters such as malondialdehyde (MDA), hydrogen peroxide (H2O2), and proline contents were determined. Both cultivars showed simil...
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In this study, enzymatic hydrolysis of corn cobs, orange and pomegranate peels were evaluated. For the hydrolysis of corn cobs, effect of alkaline pretreatment was examined and it was found that glucose and reducing sugar yield (%) for the pretreated corn cobs increased from 7% to 21.5% and 14% to 33.6% respectively. Effect of cellobiase loading on hydrolysis efficiency was also investigated. It was observed that when cellobiase was added to hydrolysate in addition to cellulase, glucose and total reducing s...
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Citation Formats
U. C. Akcay et al., “Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L.) seedlings,” PLANT GROWTH REGULATION, pp. 21–28, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36350.