Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L.) seedlings

Date

2010-05-01

Author

Akcay, U. Celikkol
Ercan, O.
Kavas, M.
Yildiz, L.
Yılmaz, Çetin
Öktem, Hüseyin Avni
YÜCEL, MUSTAFA

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

231
views

0
downloads

Two cultivars of peanut (Arachis hypogaea L.) which were designated as resistant (Florispan) and sensitive (Gazipasa) according to their growth retardation under drought stress conditions were compared for their oxidative damage and antioxidant responses. Sixteen days-old peanut seedlings were subjected to PEG-6000 solutions of two different osmotic potentials; -0.4 and -0.8 MPa, and various growth parameters, photosystem II activity, changes in malondialdehyde (MDA), hydrogen peroxide (H2O2) and proline levels, activities of ascorbate peroxidase (APX), catalase (CAT), peroxidase (POX) and gluthatione reductase (GR) enzymes were determined. Both cultivars exhibited water deficit at -0.8 MPa osmotic potential of PEG-6000 and H2O2 levels significantly increased during exposure to -0.4 MPa osmotic potential. However, H2O2 levels were under control in both cultivars at exposure to -0.8 MPa osmotic potential. Significant proline accumulation was observed in the tissues of cv. Florispan at -0.8 MPa osmotic potential, whereas proline accumulation did not appear to be an essential part of the protection mechanism against drought in cv. Gazipasa. No significant variation in chlorophyll fluorescence values were detected in neither of the cultivars. Enzyme activity measurements revealed that Gazipasa copes well with lesser magnitudes of drought stress by increasing the activity of mainly APX, and during harsh stress conditions, only APX maintains its activity in the tissues. In cultivar Florispan, GR activity appears to take role in lesser magnitudes of drought stress, whereas CAT and APX activities appear to be very crucial antioxidative defenses during intense stress conditions. The results indicate that, the level of proline and activities of the enzymes CAT and APX are important mechanisms for the maintenance of drought tolerance in peanut plants.

Subject Keywords

Arachis hypogaea, Drought stress, Antioxidant enzymes, Proline, Lipid peroxidation, Oxidative stress

URI

https://hdl.handle.net/11511/36350

Journal

PLANT GROWTH REGULATION

DOI

https://doi.org/10.1007/s10725-010-9445-1

Collections

Department of Civil Engineering, Article

Suggestions

OpenMETU
Core

ANTIOXIDANT RESPONSES OF PEANUT (ARACHIS HYPOGAEA L.) SEEDLINGS TO PROLONGED SALT-INDUCED STRESS KAVAS, MUSA; Akca, Oya Ercan; Akcay, Ufuk Celikkol; Peksel, Begum; Eroğlu, Seçkin; Öktem, Hüseyin Avni; Yucel, Meral (2015-01-01) In this study, the effects of long-term NaCl treatment were investigated in two cultivars of peanut designated as drought-resistant and drought-sensitive. Growth parameters, changes in the concentrations of MDA, H2O2 and proline, and the activities of antioxidant enzymes were determined under salinity stress. Growth parameters indicated the superiority of cv. Florispan to cv. Gazipasa under milder salinity stress treatment. However, comparative analysis of the two cultivars showed that MDA, H2O2, ion leakag...
Antioxidative and physiological responses of two sunflower (Helianthus annuus) cultivars under PEG-mediated drought stress Baloglu, Mehmet Cengiz; Kavas, Musa; Aydin, Gulsum; Öktem, Hüseyin Avni; Yücel, Ayşe Meral (2012-01-01) Drought stress is one of the most important yield-reducing factors in crop production. Sunflower, an oilseed crop, is severely affected by abiotic stress. In this study, 2 sunflower cultivars (Musala and Aydin) were evaluated in terms of various biochemical and physiological responses under 2 different polyethylene glycol-mediated drought stress conditions. Stress-determining parameters such as malondialdehyde (MDA), hydrogen peroxide (H2O2), and proline contents were determined. Both cultivars showed simil...
Antioxidant responses of shoots and roots of lentil to NaCl-salinity stress Bandeoglu, E; Eyidogan, F; Yucel, M; Öktem, Hüseyin Avni (2004-01-01) The effect of salt stress (100 mM and 200 mM NaCl) on antioxidant responses in shoots and roots of 14-day-old lentil (Lens culinaris M.) seedlings was investigated. Salt stress caused a significant decrease in length, wet-dry weight and an increase in proline content of both shoot and root tissues. In leaf tissues, high salinity treatment resulted in a 4.4 fold increase in H2O2 content which was accompanied by a significant level of lipid peroxidation and an increase in electrolyte leakage. Root tissues wer...
Antioxidant responses of lentil to cold and drought stress Öktem, Hüseyin Avni; Demirba, Didem; Bayrac, Abdullah Tahir; Oez, Mehmet Tufan; Oezguer, Ebru; Selcuk, Feyza; Yuecel, Meral (2008-01-01) The effects of cold and drought stress on antioxidant responses and growth parameters in shoots and roots of lentil (Lens culinaris M cv Sultan 1) seedlings were investigated. Ten-day-old hydroponically grown seedlings were subjected to drought and cold (4 degrees C) stress for 5 days. The length and fresh weight of shoots decreased significantly under both stress conditions, contrary to the increase in these growth parameters for roots under the same conditions. The oxidative damage as:generation of malond...
Physiological, biochemical and molecular analysis of wheat cultivars under boron treatments Kayıhan, Ceyhun; Yücel, Ayşe Meral; Eyidoğan, Füsun; Department of Biotechnology (2014) Many studies have described the physiological, biochemical and molecular responses to boron (B) toxicity and deficiency individually in plants. However, overall pattern of gene expression changes and their physiological and biochemical signiﬁcances after high B, low B and supplementing artificial B-enriched fertilizer (Tarımbor) application have not been proposed up till now. In the present study, we assessed global changes in gene expression of two wheat cultivars Bolal-2973 and Atay-85 differing in their ...

Citation Formats

U. C. Akcay et al., “Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L.) seedlings,” PLANT GROWTH REGULATION, pp. 21–28, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36350.