In vivo distribution of beta 2 glycoprotein I under various pathophysiologic conditions

Date

2011-10-13

Author

Agostinis, Chiara
BİFFİ, Stefania
GARROVO, Chiara
DURİGUTTO, Paolo
LORENZON, Andrea
Bek, Alpan
Bulla, Roberta
Grossi, Claudia
Borghi, Maria O.
Meroni, PierLuigi
Tedesco, Francesco

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In vitro studies have documented beta 2 glycoprotein I (beta 2GPI) binding to endothelial cells (ECs) and trophoblast using antiphospholipid antibodies. The in vivo binding of beta 2GPI to these cells and the conditions that favor their interaction have not been investigated. We analyzed the in vivo distribution of cyanine 5.5-labeled beta 2GPI in mice and evaluated the effect of pregnancy and circulating antibodies on its tissue localization. The signal was detected in the liver by whole body scan and ex vivo analysis. The beta 2GPI failed to bind to the vascular endothelium and reacted only with the ECs of uterine vessels. In pregnant mice the protein was localized on ECs and trophoblast at the embryo implantation sites. Immunized mice showed a similar beta 2GPI biodistribution to naive mice but the immunized pregnant animals exhibited a significant increase in fetal loss associated with C3 and C9 deposition at the implantation sites. Treatment of mice with LPS after beta 2GPI-Cy5.5 injection promoted protein localization on gut and brain ECs associated with IgG, C1q, and C9 deposition in immunized mice. These findings indicate that beta 2GPI binding to EC requires priming with pro-inflammatory factors which is not needed for uterine and placental localization probably dependent on hormonal changes. (Blood. 2011;118(15):4231-4238)

Subject Keywords

Definite antiphospholipid syndrome, International consensus statement, Induced fetal loss, Beta(2)-glycoprotein i, Tissue factor, Anti-beta(2)-glycoprotein-i antibodies, Classification criteria, Endothelial activation, Complement activation, Mediated thrombosis

URI

https://hdl.handle.net/11511/36358

Journal

BLOOD

DOI

https://doi.org/10.1182/blood-2011-01-333617

Collections

Department of Physics, Article

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Citation Formats

C. Agostinis et al., “In vivo distribution of beta 2 glycoprotein I under various pathophysiologic conditions,” BLOOD, pp. 4231–4238, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36358.