Glucose isomerase production on a xylan-based medium by Bacillus thermoantarcticus

Date

2009-01-15

Author

Çalık, Pınar
Haykir, Nazife Isik
BOYACI, İSMAİL HAKKI

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Effects of medium components on intracellular glucose isomerase (GI) production were investigated by Bacillus thermoantarcticus. The highest GI activity was obtained as 1630 U dm(-3) in the medium containing (g dm(-3)): 10.6, birchwood-xylan: 5.6, yeast extract: 5.9 (NH(4))(2)SO(4) at T = 55 C in 33 cm(-3) shake-flasks. When birchwood-xylan was replaced with oat spelt- or beechwood-xylan, GI activity decreased to 1372 and 1308 U dm(-3). respectively. Effects of pH at uncontrolled-pH (pH(UC) = 6.0) and controlled-pH (pH(C) = 6.0) operations, and oxygen transfer at the all, inlet rate of 0.5vvm and agitation rates of 300, 500 and 700 min(-1), were investigated in 3.0 dm(-3) bioreactor system with 1.65 dm(3) working Volume in the designed medium. The highest GI activity was attained at 500 min(-1), 0.5 vvm, pH(UC) = 6 as 1840 U dm(-3) where cell concentration was 2.3 g dm(-3). The use of agricultural waste xylan, as the carbon source resulted in concomitant production of xylanase and GI. The highest xylanase activity was attained as 9300 U dm(-3) at 500 min(-1) and 0.5 vvm. K(L)a varied between 0.008-0.033 s(-1) whereas the highest oxygen Uptake Fate was 0.002 mmol dm(-3) s(-1). Initially biochemical reaction limitations were effective: thereafter, mass transfer resistances became more effective,

Subject Keywords

Biotechnology, Environmental Engineering, Bioengineering, Biomedical Engineering

URI

https://hdl.handle.net/11511/36537

Journal

BIOCHEMICAL ENGINEERING JOURNAL

DOI

https://doi.org/10.1016/j.bej.2008.08.003

Collections

Department of Chemical Engineering, Article

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Citation Formats

P. Çalık, N. I. Haykir, and İ. H. BOYACI, “Glucose isomerase production on a xylan-based medium by Bacillus thermoantarcticus,” BIOCHEMICAL ENGINEERING JOURNAL, pp. 8–15, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36537.