Low-dose curcumin reduced TNBS-associated mucin depleted foci in mice by scavenging superoxide anion and lipid peroxides, rebalancing matrix NO synthase and aconitase activities, and recoupling mitochondria

Date

2020-08-01

Author

Mouzaoui, Souad
Banerjee, Sreeparna
Djerdjouri, Bahia

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

152
views

0
downloads

Background The role of mitochondrial dysfunction in the pathogenesis of inflammatory bowel diseases (IBD) is still being investigated. This study evaluated the therapeutic effect of curcumin (Cur), a polyphenolic electrophile in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced chronic colitis and mitochondrial dysfunction, in mice. Methods Colitis was induced by rectal instillation to mice of 30 mg kg(-1)TNBS, alone or followed by daily intraperitoneal injections of Cur 25 mg kg(-1). Animals were euthanized at days 3, 7, and 14, post TNBS challenge. Colon mitochondria of control mice were treated with 5 mu M Cur, and TNBS (50, 100 mu M)-toxicity was evaluated by measuring swelling, respiration, and aconitase and fumarase activities. Redox status was evaluated in colon mucosa and in mitochondria. Results In vitro, a short-term Cur treatment controlled the dose and time dependent mitochondrial toxicity induced by TNBS, by collapsing the generation of superoxide anion and hydroperoxy lipids, rebalancing nitric oxide synthase and aconitase activities, and recoupling mitochondria. In vivo, a daily low-dose Cur abolished mice mortality which reached 27% in model group. Cur improved in a time dependent manner mucosal redox homeostasis, cell apoptosis, mucin depleted crypts and crypt abscesses by controlling prooxidant activity of myeloperoxidase and NO synthase associated to phagocytes influx, quenching hydroperoxy lipids, and reboosting GSH levels. Conclusion Cur, by quenching intra and extra mitochondrial ROS generation, rebalancing aconitase/fumarase and MDA/GSH ratios, and recoupling mitochondria, may support mithormesis priming and remitting in IBD. Graphic abstract

Subject Keywords

Immunology, Pharmacology (medical), Pharmacology, Aconitase, Curcumin, Electrophile, MDA/GSH, Mucin depleted crypts, TNBS

URI

https://hdl.handle.net/11511/36714

Journal

INFLAMMOPHARMACOLOGY

DOI

https://doi.org/10.1007/s10787-019-00684-4

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Interaction of Intestinal Microbiota and Intestinal Epithelial Health in High Carbohydrate Diet Ulutaş, Mehmet Sefa; Gültekin, Güzin Candan; Aydın, Aysun Cebeci; Department of Biotechnology (2021-8) Inflammatory bowel diseases (IBDs) are chronic inflammatory disorders that cause prolonged inflammation in the gastrointestinal tract. In western countries high fat and protein consumption is shown as the main cause of inflammatory bowel diseases. However, traditional Turkish diet heavily relies on carbohydrate-based foods, the incidence of IBD in Turkey is very similar to western countries. We hypothesize that this may be due to high carbohydrate consumption. In the first part of the study, the interactio...
Regulation of Glutathione S-Transferase Mu with type 1 diabetes and its regulation with antioxidants SADİ, GÖKHAN; Kartal, Deniz Irtem; Güray, Nülüfer Tülün (2013-01-01) Objective: Increased oxidative stress is now related with the pathogenesis and the chronic complications associated with the disease, diabetes mellitus. While roles of oxidative stress in diabetic complications are widely studied, the molecular mechanisms playing role in the regulations of detoxification enzymes in the presence of antioxidants have not been clearly established because of the complexity of the pathways.
The serum angiotensin converting enzyme and lysozyme levels in patients with ocular involvement of autoimmune and infectious diseases Sahin, Ozlem; Ziaei, Alireza; Karaismailoğlu, Eda; Taheri, Nusret (Springer Science and Business Media LLC, 2016-2-16) Background: Increased serum levels of angiotensin converting enzyme and lysozyme are considered as inflammatory markers for diagnosis of sarcoidosis which is an autoimmune inflammatory disease. The purpose of this study is to evaluate the significance of differences in serum angiotensin converting enzyme and lysozyme levels of patients with ocular involvement of other autoimmune inflammatory and infectious diseases. Methods: This is a prospective study involving patients with ankylosing spondylitis, behc...
Improving the Computer-Aided Estimation of Ulcerative Colitis Severity According to Mayo Endoscopic Score by Using Regression-Based Deep Learning Polat, Gorkem; Kani, Haluk Tarik; Ergenc, Ilkay; Alahdab, Yesim Ozen; Temizel, Alptekin; Atug, Ozlen (2022-11-01) Background Assessment of endoscopic activity in ulcerative colitis (UC) is important for treatment decisions and monitoring disease progress. However, substantial inter- and intraobserver variability in grading impairs the assessment. Our aim was to develop a computer-aided diagnosis system using deep learning to reduce subjectivity and improve the reliability of the assessment. Methods The cohort comprises 11 276 images from 564 patients who underwent colonoscopy for UC. We propose a regression-based deep ...
Impaired toll like receptor-7 and 9 induced immune activation in chronic spinal cord injured patients contributes to immune dysfunction Gucluler, Gozde; Adiguzel, Emre; Gungor, Bilgi; Kahraman, Tamer; Gürsel, Mayda; Yilmaz, Bilge; GÜRSEL, İHSAN (Public Library of Science (PLoS), 2017-02-07) Reduced immune activation or immunosuppression is seen in patients withneurological diseases. Urinary and respiratory infections mainly manifested as septicemia and pneumonia are the most frequent complications following spinal cord injuries and they account for the majority of deaths. The underlying reason of these losses is believed to arise due to impaired immune responses to pathogens. Here, we hypothesized that susceptibility to infections of chronic spinal cord injured (SCI) patients might be due to i...

Citation Formats

S. Mouzaoui, S. Banerjee, and B. Djerdjouri, “Low-dose curcumin reduced TNBS-associated mucin depleted foci in mice by scavenging superoxide anion and lipid peroxides, rebalancing matrix NO synthase and aconitase activities, and recoupling mitochondria,” INFLAMMOPHARMACOLOGY, pp. 949–965, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36714.