Various applications of immobilized glucose oxidase and polyphenol oxidase in a conducting polymer matrix

Date

2007-06-01

Author

Cil, M.
Boyukbayram, A. E.
Kiralp, S.
Toppare, Levent Kamil
Yagci, Y.

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In this study, glucose oxidase and polyphenol oxidase were immobilized in conducting polymer matrices; polypyrrole and poly(N-(4-(3-thienyl methylene)-oxycarbonyl phenyl) maleimide-co-pyrrole) via electrochemical method. Fourier transform infrared and scanning electron microscope were employed to characterize the copolymer of (N-(4-(3-thienyl methylene)-oxycarbonyl phenyl) maleimide) with pyrrole. Kinetic parameters, maximum reaction rate and Michealis-Menten constant, were determined. Effects of temperature and pH were examined for immobilized enzymes. Also, storage and operational stabilities of enzyme electrodes were investigated. Glucose and polyphenol oxidase enzyme electrodes were used for determination of the glucose amount in orange juices and human serum and phenolic amount in red wines, respectively.

Subject Keywords

Biochemistry, Molecular Biology, Structural Biology, General Medicine

URI

https://hdl.handle.net/11511/35475

Journal

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

DOI

https://doi.org/10.1016/j.ijbiomac.2006.12.005

Collections

Department of Chemistry, Article

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Citation Formats

M. Cil, A. E. Boyukbayram, S. Kiralp, L. K. Toppare, and Y. Yagci, “Various applications of immobilized glucose oxidase and polyphenol oxidase in a conducting polymer matrix,” INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, pp. 49–55, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35475.