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Carboligation reactions with benzaldehyde lyase immobilized on superparamagnetic solid support

2009-01-01
Sopaci, S. Betuel
Turan, Ilke Simsek
Tural, Bilsen
Volkan, Mürvet
Demir, Ayhan Sıtkı
Histidine-tagged recombinant benzaldehyde lyase (BAL, EC 4.1.2.38) was efficiently immobilized to surface-modified magnetic particles with affinity ligand binding. In addition to conventional benzoin condensation reactions, two important representative BAL-catalyzed carboligation reactions, were also performed with this magnetically responsive biocatalyst. The results obtained from the carboligation reactions that were performed with this simple and convenient heterogenous biocatalyst were comparable to that of free-enzyme-catalyzed reactions.