Glucose determination based on a two component self-assembled monolayer functionalized surfaceenhanced Raman spectroscopy (SERS) probe

Dudak, Fahriye Ceyda
Adiguzel, Yekbun
Külah, Haluk
In this report, we present a new detection method for blood glucose, using gold nanorod SERS, a surface enhanced Raman scattering probe embedded in two component self-assembled monolayers (SAMs). Gold nanorod particles and a gold coated slide surface were modified with the two component SAMs consisting of 3-mercaptophenylboronic acid (3-MBA) and 1-decanethiol (1-DT). The immobilization of 3-MBA/1-DT surface-functionalized gold nanoparticles onto 3-MBA/1-DT modified gold-coated slide surfaces was achieved by the cooperation of hydrophobic forces. Two component SAM functionalized substrates were used as SERS probes, by means of the boronic acid and the alkyl spacer functional groups that serve as the molecular recognition and penetration agents, respectively. The SERS platform surface was characterized by cyclic voltammetry, contact angle measurements, AFM (atomic force microscopy) and Raman spectroscopy. Optimum values of the parameters such as pH, time and (3-MBA/1-DT) molar ratio were also examined for the glucose determination. The analytical performance was evaluated and linear calibration graphs were obtained in the glucose concentration range of 2-16 mM, which is also in the range of the blood glucose levels, and the detection limit was found to be 0.5 mM. As a result, the SERS platform was also used for the determination of glucose in plasma samples.


Glucose electrooxidation modelling studies on carbon nanotube supported Pd catalyst with response surface methodology and density functional theory
KAYA, ŞEFİKA; Ulaş, Berdan; Duzenli, Derya; Önal, Işık; Er, Omer Faruk; Yilmaz, Yonca; Tezsevin, Ilker; DEMİR KIVRAK, HİLAL (2022-09-01)
© 2022 Elsevier LtdIn this study, carbon nanotube supported Pd catalysts (Pd/CNT) are synthesized at different weight percentages by the sodium borohydride (NaBH4) reduction method to investigate catalytic performance of glucose electrooxidation reaction. 0.5% Pd/CNT, 3% Pd/CNT, and 7% Pd/CNT catalysts are characterized by using X-ray diffraction (XRD), electron microscopy with energy dispersive X-ray (SEM-EDX), and N2 adsorption-desorption measurements. The average particle size and surface area of 3% Pd/C...
Laser induced periodic surface structuring for surface enhanced raman spectroscopy
Özkarslıgil, Zeynep Tuğçe; Bek, Alpan; Department of Physics (2020)
In this study, our aim is to fabricate and characterize efficient substrates of surface enhanced Raman spectroscopy (SERS) utilizing the field enhancement due to hot spots made by recently developed method of laser induced periodic surface structuring (LIPSS). LIPSS is a cost-effective technique for rapid processing of almost any materials compared to conventional lithography methods. Coating of a thin silver film on LIPSS applied substrate surface provides to observe the localized surface plasmon effect fo...
Facile fabrication of Au-Ag alloy nanoparticles on filter paper: Application in SERS based swab detection and multiplexing
Khan, Ghazanfar Ali; Demirtaş, O. Özge; Bek, Alpan; Bhatti, Arshad Saleem; Ahmed, Waqqar (2022-05-01)
© 2022 Elsevier B.V.Facile fabrication of flexible substrates containing high concentration of nanoparticles (NPs) is very promising owing to their capability of swab-based surface-enhanced Raman scattering (SERS) trace detection. However, the background signal of the substrate could compromise the trace-detection capabilities. Moreover, the presence of fluorescent molecules may result in intense fluorescence background which could overshadow the Raman peaks. Herein, we demonstrate that the surfactant-free ...
Production and characterisation of PMN-PT piezoceramics modified by yttrium and bismuth
Camurlu, HE; Timucin, M (2004-01-01)
This study was carried out to investigate the effects of Bismuth (Bi+3) and Yttrium (y(+3)) on the dielectric and piezoelectric properties of Lead Magnesium Niobate-Lead Titanate (PMN-PT) compositional system. The ceramic powders were produced by thermal synthesis using a modified mixed oxide technique. The sintering parameters were optimized for developing piezoelectric ceramics with enhanced piezoelectric strain coefficients.
Gold nanoparticle modified conducting polymer of 4-(2,5-di(thiophen-2-yl)-1H-pyrrole-1-l) benzenamine for potential use as a biosensing material
Tuncagil, Sevinc; Ozdemir, Caglar; ODACI DEMİRKOL, DİLEK; TİMUR, SUNA; Toppare, Levent Kamil (2011-08-01)
Gold nanoparticle (AuNP) modified conducting polymer of 4-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine (SNS-NH2) was used as the biosensing platform for glucose analysis. Electrochemical measurements were carried out by following the consumed oxygen due to the enzymatic reaction of glucose oxidase (GOx) at -0.7 V vs Ag/AgCl. Optimisation of pH, enzyme loading, stability experiments were carried out. Effect of NP was investigated by monitoring the signal responses at different AuNP sizes and amounts. A ...
Citation Formats
H. TORUL et al., “Glucose determination based on a two component self-assembled monolayer functionalized surfaceenhanced Raman spectroscopy (SERS) probe,” ANALYTICAL METHODS, pp. 5097–5104, 2014, Accessed: 00, 2020. [Online]. Available: