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High-Throughput Screening and Quantitation of Target Compounds in Biofluids by Coated Blade Spray-Mass Spectrometry
Date
2017-08-15
Author
Tascon, Marcos
Gomez-Rios, German Augusto
Reyes-Garces, Nathaly
Poole, Justen
Boyacı, Ezel
Pawliszyn, Janusz
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Most contemporary methods of screening and quantitating controlled substances and therapeutic drugs in biofluids typically require laborious, time-consuming, and expensive analytical workflows. In recent years, our group has worked toward developing microextraction (mu e)-mass spectrometry (MS) technologies that merge all of the tedious steps of the classical methods into a simple, efficient, and low-cost methodology. Unquestionably, the automation of these technologies allows for faster sample throughput, greater reproducibility, and radically reduced analysis times. Coated blade spray (CBS) is a mu e technology engineered for extracting/enriching analytes of interest in complex matrices, and it can be directly coupled with MS instruments to achieve efficient screening and quantitative analysis. In this study, we introduced CBS as a technology that can be arranged to perform either rapid diagnostics (single-vial) or the high-throughput (96-well plate) analysis of biofluids. Furthermore, we demonstrate that performing 96-CBS extractions at the same time allows the total analysis time to be reduced to less than SS s per sample. Aiming to validate the versatility of CBS, substances comprising a broad range of molecular weights, moieties, protein binding, and polarities were selected. Thus, the high-throughput (HT)-CBS technology was used for the concomitant quantitation of 18 compounds (mixture of anabolics, beta-2 agonists, diuretics, stimulants, narcotics, and beta-blockers) spiked in human urine and plasma samples. Excellent precision (similar to 2.5%), accuracy (>= 90%), and linearity (R-2 >= 0.99) were attained for all the studied compounds, and the limits of quantitation (LOQs) were within the range of 0.1 to 10 ng.mL(-1) for plasma and 0.25 to 10 ng.mL(-1) for urine. The results reported in this paper confirm CBS's great potential for achieving subsixty-second analyses of target compounds in a broad range of fields such as those related to clinical diagnosis, food, the environment, and forensics.
Subject Keywords
Analytical Chemistry
URI
https://hdl.handle.net/11511/37278
Journal
ANALYTICAL CHEMISTRY
DOI
https://doi.org/10.1021/acs.analchem.7b01877
Collections
Department of Chemistry, Article
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M. Tascon, G. A. Gomez-Rios, N. Reyes-Garces, J. Poole, E. Boyacı, and J. Pawliszyn, “High-Throughput Screening and Quantitation of Target Compounds in Biofluids by Coated Blade Spray-Mass Spectrometry,”
ANALYTICAL CHEMISTRY
, pp. 8421–8428, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37278.