Fermentation and oxygen transfer characteristics in serine alkaline protease production by recombinant Bacillus subtilis in molasses-based complex medium

Date

2004-11-01

Author

Calik, G
Pehlivan, N
Ozcelik, IS
Çalık, Pınar
Ozdamar, TH

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

60
views

0
downloads

Serine alkaline protease (SAP) production in a complex medium based on physically pretreated molasses by recombinant Bacillus subtilis carrying pHV1431::subC gene is described. The effects of oxygen transfer were investigated in 3.5 dm(3) bioreactor systems with controls for agitation rate, dissolved oxygen, pH, temperature, and foam formation under two different agitation rates, ie N = 500 and 750 min(-1), and four different air flow rates, ie Q/V-R = 0.2, 0.5, 0.7, and 1.0 vvm, at a molasses concentration equivalent to initial sucrose concentration (C-So) of 20 kg m(-3). The yield values (Y-x/s, Y-x/o, Y-s/o) and maintenance coefficient of oxygen (m(o)), were calculated. mo decreased with the increase in the air-inlet rate. Increase in oxygen transfer rate increased the rate of growth and SAP activity, and affected the cultivation time to achieve maximum expression of SAP activity. At Q/V-R = 0.5 vvm and N = 750 min(-1), SAP activity reached 2250 U cm(-3) at t = 36 h. The oxygen transfer coefficient (K(L)a) and oxygen uptake rate (-r(o)) were measured throughout the fermentations and their variation with the oxygen transfer conditions determined. New correlations for the calculation of KLa and -r(o) are proposed. (C) 2004 Society of Chemical Industry.

Subject Keywords

Biotechnology, Fuel Technology, Inorganic Chemistry, Renewable Energy, Sustainability and the Environment, Organic Chemistry, Waste Management and Disposal, Pollution, General Chemical Engineering

URI

https://hdl.handle.net/11511/39291

Journal

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

DOI

https://doi.org/10.1002/jctb.1118

Collections

Department of Chemical Engineering, Article

Suggestions

OpenMETU
Core

Metabolic reaction network of Pichia pastoris with glycosylation reactions: Flux analysis for erythropoietin production Eskitoros, Melda S.; Ata, Ozge; Çalık, Pınar (Wiley, 2014-11-01) BACKGROUND: Biochemical reaction network of Pichia pastoris was improved by including N-glycosylation pathway reactions to determine the intracellular reaction rates for glycosylated protein production.
Beet molasses based exponential feeding strategy for thermostable glucose isomerase production by recombinant Escherichia coli BL21 (DE3) Angardi, Vahideh; Çalık, Pınar (Wiley, 2013-05-01) BACKGROUND: The effects of pretreated beet molasses based feeding strategies on thermostable glucose isomerase (GI) production by recombinant Escherichia coli BL21 (DE3) pLysS were investigated. RESULTS: The thermostable GI encoding gene of Thermus thermophilus (xylA) was recombined with pRSETA vector, and the pRSETA::xylA obtained was transferred into E.coli BL21 (DE3) pLysS and used for GI production. The highest soluble GI activity was obtained at t = 30 h, as A = 16 400 U L1 (20.6 U mg1 protein) under m...
Fermentation and oxygen transfer characteristics in recombinant human growth hormone production by Pichia pastoris in sorbitol batch and methanol fed-batch operation Çalık, Pınar; Soyaslan, Elif S.; Sahin, Merve; Taspinar, Hatice; Acik, Eda; Bayraktar, Eda (Wiley, 2010-02-01) BACKGROUND: The influence of methanol feed rate on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) in medium containing sorbitol was investigated at three different specific growth rates (mu), namely, 0.02 (MS-0.02), 0.03 (MS-0.03), and 0.04 (MS-0.04).
Oxygen transfer effects in beta-lactamase fermentation by Bacillus licheniformis in a glucose-based defined medium Çalık, Pınar; Calik, G (Wiley, 2005-09-01) The effects of oxygen transfer on the beta-lactamase production by Bacillus licheniformis were investigated in a glucose-based defined medium. The experiments were conducted in 3.0 dm(3) batch bioreactor systems at three different air inlet (Q(O)/V-R = 0.2, 0.5 and 1.0 vvm) and agitation rates (N = 250, 500 and 750 min(-1)). During the fermentation, the concentrations of the cell, glucose, by-products, ie organic and amino acids, oxygen transfer coefficients (K(L)alpha), yield coefficients, specific rates a...
Enzymic activity of the K5-type yeast killer toxin and its characterization Izgu, F; Altinbay, D; Sertkaya, A (Informa UK Limited, 2005-11-01) K5-type yeast killer toxin secreted by P. anomala NCYC 434 cells has a broad killing spectrum. Competitive inhibiton of killer activity showed that glucans, mainly the beta-1,3 glucan, represent the primary toxin binding site within the cell wall of sensitive cells. Its hydrolytic activity on laminarin in an exo-like fashion revealed that the toxin exerts its killing effect by exo-beta-1,3-glucanase activity. Its specific activity on laminarin was 120U/mg, and the Michaelis constants K-m, and V-max for lami...

Citation Formats

G. Calik, N. Pehlivan, I. Ozcelik, P. Çalık, and T. Ozdamar, “Fermentation and oxygen transfer characteristics in serine alkaline protease production by recombinant Bacillus subtilis in molasses-based complex medium,” JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, pp. 1243–1250, 2004, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39291.