Show/Hide Menu
Hide/Show Apps
anonymousUser
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Videos
Videos
Thesis submission
Thesis submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Contact us
Contact us
Fermentation and oxygen transfer characteristics in serine alkaline protease production by recombinant Bacillus subtilis in molasses-based complex medium
Date
2004-11-01
Author
Calik, G
Pehlivan, N
Ozcelik, IS
Çalık, Pınar
Ozdamar, TH
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
5
views
0
downloads
Cite This
Serine alkaline protease (SAP) production in a complex medium based on physically pretreated molasses by recombinant Bacillus subtilis carrying pHV1431::subC gene is described. The effects of oxygen transfer were investigated in 3.5 dm(3) bioreactor systems with controls for agitation rate, dissolved oxygen, pH, temperature, and foam formation under two different agitation rates, ie N = 500 and 750 min(-1), and four different air flow rates, ie Q/V-R = 0.2, 0.5, 0.7, and 1.0 vvm, at a molasses concentration equivalent to initial sucrose concentration (C-So) of 20 kg m(-3). The yield values (Y-x/s, Y-x/o, Y-s/o) and maintenance coefficient of oxygen (m(o)), were calculated. mo decreased with the increase in the air-inlet rate. Increase in oxygen transfer rate increased the rate of growth and SAP activity, and affected the cultivation time to achieve maximum expression of SAP activity. At Q/V-R = 0.5 vvm and N = 750 min(-1), SAP activity reached 2250 U cm(-3) at t = 36 h. The oxygen transfer coefficient (K(L)a) and oxygen uptake rate (-r(o)) were measured throughout the fermentations and their variation with the oxygen transfer conditions determined. New correlations for the calculation of KLa and -r(o) are proposed. (C) 2004 Society of Chemical Industry.
Subject Keywords
Biotechnology
,
Fuel Technology
,
Inorganic Chemistry
,
Renewable Energy, Sustainability and the Environment
,
Organic Chemistry
,
Waste Management and Disposal
,
Pollution
,
General Chemical Engineering
URI
https://hdl.handle.net/11511/39291
Journal
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
DOI
https://doi.org/10.1002/jctb.1118
Collections
Department of Chemical Engineering, Article
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
G. Calik, N. Pehlivan, I. Ozcelik, P. Çalık, and T. Ozdamar, “Fermentation and oxygen transfer characteristics in serine alkaline protease production by recombinant Bacillus subtilis in molasses-based complex medium,”
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
, vol. 79, no. 11, pp. 1243–1250, 2004, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39291.