Metabolic reaction network of Pichia pastoris with glycosylation reactions: Flux analysis for erythropoietin production

2014-11-01
Eskitoros, Melda S.
Ata, Ozge
Çalık, Pınar
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
231
views
0
downloads
BACKGROUND: Biochemical reaction network of Pichia pastoris was improved by including N-glycosylation pathway reactions to determine the intracellular reaction rates for glycosylated protein production.
Biotechnology, Fuel Technology, Inorganic Chemistry, Renewable Energy, Sustainability and the Environment, Organic Chemistry, Waste Management and Disposal, Pollution, General Chemical Engineering
https://hdl.handle.net/11511/39890
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
Department of Chemical Engineering, Article

Suggestions

Fermentation and oxygen transfer characteristics in recombinant human growth hormone production by Pichia pastoris in sorbitol batch and methanol fed-batch operation
Çalık, Pınar; Soyaslan, Elif S.; Sahin, Merve; Taspinar, Hatice; Acik, Eda; Bayraktar, Eda (Wiley, 2010-02-01)
BACKGROUND: The influence of methanol feed rate on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) in medium containing sorbitol was investigated at three different specific growth rates (mu), namely, 0.02 (MS-0.02), 0.03 (MS-0.03), and 0.04 (MS-0.04).
Fermentation and oxygen transfer characteristics in serine alkaline protease production by recombinant Bacillus subtilis in molasses-based complex medium
Calik, G; Pehlivan, N; Ozcelik, IS; Çalık, Pınar; Ozdamar, TH (Wiley, 2004-11-01)
Serine alkaline protease (SAP) production in a complex medium based on physically pretreated molasses by recombinant Bacillus subtilis carrying pHV1431::subC gene is described. The effects of oxygen transfer were investigated in 3.5 dm(3) bioreactor systems with controls for agitation rate, dissolved oxygen, pH, temperature, and foam formation under two different agitation rates, ie N = 500 and 750 min(-1), and four different air flow rates, ie Q/V-R = 0.2, 0.5, 0.7, and 1.0 vvm, at a molasses concentration...
Beet molasses based exponential feeding strategy for thermostable glucose isomerase production by recombinant Escherichia coli BL21 (DE3)
Angardi, Vahideh; Çalık, Pınar (Wiley, 2013-05-01)
BACKGROUND: The effects of pretreated beet molasses based feeding strategies on thermostable glucose isomerase (GI) production by recombinant Escherichia coli BL21 (DE3) pLysS were investigated. RESULTS: The thermostable GI encoding gene of Thermus thermophilus (xylA) was recombined with pRSETA vector, and the pRSETA::xylA obtained was transferred into E.coli BL21 (DE3) pLysS and used for GI production. The highest soluble GI activity was obtained at t = 30 h, as A = 16 400 U L1 (20.6 U mg1 protein) under m...
EFFECTS OF SUBSTRATE CONCENTRATION ON GROWTH AND LACTIC-ACID PRODUCTION BY MIXED CULTURES OF LACTOBACILLUS-BULGARICUS AND STREPTOCOCCUS-THERMOPHILUS
OZEN, S; OZILGEN, M (Wiley, 1992-01-01)
The kinetics of growth and lactic acid production by mixed cultures of Lactobacillus bulgaricus and Streptococcus thermophilus were modeled with a set of modified logistic and Luedeking-Piret equations. The specific growth rates and the inhibitory effect of each individual species on the mixed culture growth were found to be determined by the media composition. The kinetic analysis clearly showed that the contribution of each microbial species to the mixed culture growth process changed drastically when the...
Immobilization of invertase and glucose oxidase in conducting H-type polysiloxane/polypyrrole block copolymers
Gursel, A; Alkan, S; Toppare, Levent Kamil; Yagci, Y (Elsevier BV, 2003-01-01)
In this study, immobilizations of enzymes, invertase and glucose oxidase, were achieved in conducting copolymers of N-pyrrolyl terminated polydimethylsiloxane/polypyrrole (PDMS/PPy) matrices via electrochemical polymerization. The kinetic parameters, v(max) (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on enzyme activity and film morphologies was examined. ...
Citation Formats
M. S. Eskitoros, O. Ata, and P. Çalık, “Metabolic reaction network of Pichia pastoris with glycosylation reactions: Flux analysis for erythropoietin production,” JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, pp. 1675–1685, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39890.
METU IIS - Integrated Information Service open@metu.edu.tr