Identification of XRCC1 Arg399Gln and XRCC3 Thr241Met Polymorphisms in a Turkish Population and Their Association with the Risk of Chronic Lymphocytic Leukemia

Date

2015-09-01

Author

Mutlu, Pelin
Yildirim, Murat
Nevruz, Oral
Cetin, Ahmet Turker
Avcu, Ferit

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

159
views

0
downloads

DNA repair systems are essential for cellular functions. Defects due to sequence variations in DNA repair genes can lead severe failure of cell functions and causing many cancer types including leukemia. The aim of this study was to investigate the relationship between XRCC1 Arg399Gln and XRCC3 Thr241Met polymorphisms and susceptibility to chronic lymphocytic leukemia (CLL) in Turkish patients. In addition, genotype distribution of these polymorphisms was compared with other populations. The frequencies of Arg399Gln and Thr241Met single nucleotide polymorphisms were studied in 25 CLL patients and 30 healthy individuals. Single nucleotide polymorphisms were genotyped by PCR-RFLP method. The genotype and allele frequencies of Arg399Gln and Thr241Met polymorphisms were not statistically different between the CLL patients and control group. The allelic frequency similarities were found between Turkish and Brazilian populations for Arg399Gln polymorphism. On the other hand, similarities were found between Turkish and other Caucasian populations for Thr241Met polymorphism. Marked differences were observed between American African versus Turkish and Chinese versus Turkish populations for Arg399Gln and Thr241Met polymorphisms respectively. These results indicate that Arg399Gln and Thr241Met polymorphisms were not associated with the development of CLL in Turkish population and ethnic differences is one of the most important factor for allele frequency differences.

Subject Keywords

Chronic lymphocytic leukemia, SNP, XRCC1, XRCC3

URI

https://hdl.handle.net/11511/37834

Journal

INDIAN JOURNAL OF HEMATOLOGY AND BLOOD TRANSFUSION

DOI

https://doi.org/10.1007/s12288-014-0482-1

Collections

Test and Measurement Center In advanced Technologies (MERKEZ LABORATUVARI), Article

Suggestions

OpenMETU
Core

Association between XRCC3 Thr241Met polymorphism and laryngeal cancer susceptibility in Turkish population Mutlu, Pelin; Yalcin, Serap; Yaylaci, Atilay; Unsoy, Gozde; Saylam, Guleser; Akm, Istemihan; Gündüz, Ufuk (2015-12-01) DNA repair systems are essential for normal cell function. Genetic alterations in the DNA repair genes such as X-ray repair cross-complementing group 3 (XRCC3), can cause a change in protein activity which results in cancer susceptibility. The aim of this study was to investigate the association of XRCC3 Thr241Met single nucleotide polymorphism (SNP), smoking and alcohol consumption with the risk of laryngeal cancer in Turkish population. The frequencies of Thr241Met SNP were studied in 58 laryngeal cancer ...
Detection of XRCC1 gene polymorphisms in Turkish head and neck squamous cell carcinoma patients: a comparative analysis with different populations Mutlu, Pelin; Yalcin, Serap; Unsoy, Gozde; Yaylaci, Atilay; Saylam, Guleser; Akin, Istemihan; Korkmaz, Hakan; Gündüz, Ufuk (2015-03-01) Purpose: X-ray repair cross-complementing (XRCC1) is one of the most important genes for the maintenance of genomic integrity and protection of cells from DNA damage. Although tobacco and alcohol consumption are the major risk factors for the development of head and neck squamous cell carcinoma (HNSCC), sequence variation in XRCC1 gene may alter HNSCC susceptibility. Reports on the relationship between HNSCC and polymorphisms in XRCC1 gene have been inconsistent so far. The aim of this study was to investig...
Isolation of High-Quality RNA from Pichia pastoris Öztürk, Sibel; Demir, İrem; Çalık, Pınar (Wiley, 2019-12-01) Analysis of RNA structuromes provides new insights into cellular processes, enabling systems biology and biotechnology researchers to calculate promoter and terminator strengths and to directly observe how differing circuit states impact host gene expression and the burdens imposed by the circuits. Such analysis, however, is crucially dependent on the availability of highly pure, intact RNA isolated from fresh or frozen cell cultures. RNA extraction from the yeast Pichia pastoris requires specific pretreatm...
IDENTIFYING ISOFORM SWITCHES IN BREAST CANCER HENDEN, Şevki Onur; Can, Tolga; Department of Computer Engineering (2021-9-9) Characterizing the human genome's molecular functions and their variations across people is vital for understanding the cellular processes behind human genetic characteristics and diseases. With the advent of single-cell RNA sequencing (scRNA-seq), it is now possible to investigate gene expression in individual cells. Although a number of scRNA-seq bioinformatics tools are now available, many of them focus on overall gene expression levels and, as a result, often ignore heterogeneity caused by individual tr...
Capturing Wheat Phenotypes at the Genome Level Hussaın, Babar; et. al. (2022-07-04) Recent technological advances in next-generation sequencing (NGS) technologies have dramatically reduced the cost of DNA sequencing, allowing species with large and complex genomes to be sequenced. Although bread wheat (Triticum aestivum L.) is one of the world’s most important food crops, efficient exploitation of molecular marker-assisted breeding approaches has lagged behind that achieved in other crop species, due to its large polyploid genome. However, an international public–private effort spanning 9 ...

Citation Formats

P. Mutlu, M. Yildirim, O. Nevruz, A. T. Cetin, and F. Avcu, “Identification of XRCC1 Arg399Gln and XRCC3 Thr241Met Polymorphisms in a Turkish Population and Their Association with the Risk of Chronic Lymphocytic Leukemia,” INDIAN JOURNAL OF HEMATOLOGY AND BLOOD TRANSFUSION, pp. 332–338, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37834.