Phaseguide assisted liquid lamination for magnetic particle-based assays

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Date

2014-01-01

Author

Phurimsak, Chayakom
Yıldırım, Ender
Tarn, Mark D.
Trietsch, Sebaastian J
Hankemeier, Thomas
Pamme, Nicole
Vulto, Paul

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We have developed a magnetic particle-based assay platform in which functionalised magnetic particles are transferred sequentially through laminated volumes of reagents and washing buffers. Lamination of aqueous liquids is achieved via the use of phaseguide technology; microstructures that control the advancing air-liquid interface of solutions as they enter a microfluidic chamber. This allows manual filling of the device, eliminating the need for external pumping systems, and preparation of the system requires only a few minutes. Here, we apply the platform to two on-chip strategies: (i) a one-step streptavidin-biotin binding assay, and (ii) a two-step C-reactive protein immunoassay. With these, we demonstrate how condensing multiple reaction and washing processes into a single step significantly reduces procedural times, with both assay procedures requiring less than 8 seconds.

Subject Keywords

Biochemistry, Bioengineering, General Chemistry, Biomedical Engineering

URI

https://hdl.handle.net/11511/37884

Journal

LAB ON A CHIP

DOI

https://doi.org/10.1039/c4lc00139g

Collections

Department of Mechanical Engineering, Article

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Citation Formats

C. Phurimsak et al., “Phaseguide assisted liquid lamination for magnetic particle-based assays,” LAB ON A CHIP, pp. 2334–2343, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37884.