Determination of malathion and diazinon resistance by sequencing the Md alpha E7 gene from Guatemala, Colombia, Manhattan, and Thailand housefly (Musca domestica L.) strains

Date

2004-04-01

Author

Taskin, V
Kence, M
Göçmen, Birşen

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

193
views

0
downloads

Organophosphate (OP) insecticides (parathion/diazinon) resistance in housefly (Musca domestica L.) is associated with the change in carboxylesterase activity. The product of alphaE7 gene, which is a member of alpha-esterase gene cluster, is probably playing a role in detoxyfication of the xenobiotic esters. In parathion/diazinon resistant M. domestica species Gly(137) to Asp substitution was found in the active center of the product of alphaE7 gene. In malathion (an OP) resistant M. domestica strains Trp(251) to Ser substitution was identified in the active center of the MdalphaE7. In our research, to understand the allelic diversity of the MdalphaE7, the gene was partially sequenced from four different housefly strains from different localities (Guatemala, Manhattan (USA), Colombia (USA), and Thailand). It was found out that; in Thailand strain one allele has Cys residue at the position of 251, the other allele contains a Trp for the same site. In Colombia strain, one allele has Asp(137), the other allele contains a Gly residue at this point. The Manhattan and Guatemala strains have Asp(137) and Trp(251) residues on their both alleles at these two different positions.

Subject Keywords

Ester , Malathion, Allelic diversity , Active center , Gene cluster

URI

https://hdl.handle.net/11511/37927

Journal

RUSSIAN JOURNAL OF GENETICS

DOI

https://doi.org/10.1023/b:ruge.0000024974.58424.f9

Collections

Department of Basic English, Article

Suggestions

OpenMETU
Core

The genetic basis of malathion resistance in Turkish house fly (Musca domestica L.) strains and worldwide distribution of MdαE7 alleles Taşkın, Vatan; Kence, Meral; Department of Biology (2002) Organophosphate (OP) insecticides (parathion / diazinon) resistance in house fly (Musca domestica L.) is associated with the change in carboxylesterase activity. The product of aE7 gene, which is a member of oc-esterase gene cluster, is probably playing a role in detoxyfication of the xenobiotic esters. In diazinon resistant Lucilia cuprina (blowfly) and diazinon / parathion resistant M.domestica species Gly137 to Asp substitution was found in the active center of the product of aE7. This enhance the abilit...
The genetic basis of malathion resistance in housefly (Musca domestica L.) strains from Turkey Taskin, V; Kence, M (Springer Science and Business Media LLC, 2004-11-01) Organophosphate insecticide (parathion/diazinon) resistance in housefly ( Musca domestica L.) is associated with the change in carboxylesterase activity. The product of MdalphaE7 gene is probably playing a role in detoxification of xenobiotic esters. In our research, we have isolated, cloned and sequenced the MdalphaE7 gene from five different Turkish housefly strains. High doses of malathion ( 600 mug/fly) were applied in a laboratory environment for one year to Ceyhan1, Ceyhan2, Adana, and Ankara strains ...
Determination of metabolic bottlenecks using reaction engineering principles in serine alkaline protease production by recombinant bacillus sp. Telli, İlkin Ece; Çalık, Pınar; Department of Chemical Engineering (2004) In this study, firstly, bioprocess characteristics for Serine Alkaline Protease (SAP) production, using recombinant Bacillus subtilis carrying pHV1431::subC, were examined. The cell concentration, substrate concentration, SAP activity and SAP synthesis rate profiles demonstrated that the system reaches to a steady state in terms of cell growth and SAP synthesis between t=15-25 h, therefore, this time interval is appropriate to employ both metabolic flux analysis and metabolic control analysis, which apply s...
Detection of physically interacting proteins with the CC and NB-ARC domains of a putative yellow rust resistance protein, Yr10, in wheat Yildirim-Ersoy, Figen; Ridout, Christopher J.; Akkaya, Mahinur (Springer Science and Business Media LLC, 2011-08-01) Gene-for-gene (GFG) resistance is a potent defense mechanism in plants, that is mediated by resistance (R) proteins. In GFG resistance, pathogen effector or avirulence (Avr) proteins are recognised by R-proteins which initiate a series of signal transduction events that lead to hypersensitive cell death. In cereals, many R-proteins are comprised of an N-terminal coiled-coiled (CC) domain, a Nucleotide Binding (NB) domain and a Leucine Rich Repeats (LRR) region associated with effector recognition. NB-LRR im...
Identification of differentially expressed proteins in wheat after benzothiadiazole treatment Gunel, Aslihan; Asbahi, Adnan; Ozgazi, Nese; Akkaya, Mahinur (2012-12-01) The systemic acquired resistance (SAR) mechanism is stimulated by biological and chemical agents in response to pathogen infection as a part of the innate immunity response system of plants. The externally applied synthetic chemical, benzo-(1,2,3)-thiadiazole-7-carbothioic acid-S-methyl ester (BTH), is also known to induce a SAR response in plants. Studies identifying genes induced or suppressed by BTH are limited. Only few genes responding to BTH are determined. The focus of this preliminary study is to id...

Citation Formats

V. Taskin, M. Kence, and B. Göçmen, “Determination of malathion and diazinon resistance by sequencing the Md alpha E7 gene from Guatemala, Colombia, Manhattan, and Thailand housefly (Musca domestica L.) strains,” RUSSIAN JOURNAL OF GENETICS, pp. 377–380, 2004, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37927.