Determination of malathion and diazinon resistance by sequencing the Md alpha E7 gene from Guatemala, Colombia, Manhattan, and Thailand housefly (Musca domestica L.) strains

Date

2004-04-01

Author

Taskin, V
Kence, M
Göçmen, Birşen

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Organophosphate (OP) insecticides (parathion/diazinon) resistance in housefly (Musca domestica L.) is associated with the change in carboxylesterase activity. The product of alphaE7 gene, which is a member of alpha-esterase gene cluster, is probably playing a role in detoxyfication of the xenobiotic esters. In parathion/diazinon resistant M. domestica species Gly(137) to Asp substitution was found in the active center of the product of alphaE7 gene. In malathion (an OP) resistant M. domestica strains Trp(251) to Ser substitution was identified in the active center of the MdalphaE7. In our research, to understand the allelic diversity of the MdalphaE7, the gene was partially sequenced from four different housefly strains from different localities (Guatemala, Manhattan (USA), Colombia (USA), and Thailand). It was found out that; in Thailand strain one allele has Cys residue at the position of 251, the other allele contains a Trp for the same site. In Colombia strain, one allele has Asp(137), the other allele contains a Gly residue at this point. The Manhattan and Guatemala strains have Asp(137) and Trp(251) residues on their both alleles at these two different positions.

Subject Keywords

Ester , Malathion, Allelic diversity , Active center , Gene cluster

URI

https://hdl.handle.net/11511/37927

Journal

RUSSIAN JOURNAL OF GENETICS

DOI

https://doi.org/10.1023/b:ruge.0000024974.58424.f9

Collections

Department of Basic English, Article

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The genetic basis of malathion resistance in Turkish house fly (Musca domestica L.) strains and worldwide distribution of MdαE7 alleles Taşkın, Vatan; Kence, Meral; Department of Biology (2002) Organophosphate (OP) insecticides (parathion / diazinon) resistance in house fly (Musca domestica L.) is associated with the change in carboxylesterase activity. The product of aE7 gene, which is a member of oc-esterase gene cluster, is probably playing a role in detoxyfication of the xenobiotic esters. In diazinon resistant Lucilia cuprina (blowfly) and diazinon / parathion resistant M.domestica species Gly137 to Asp substitution was found in the active center of the product of aE7. This enhance the abilit...
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Citation Formats

V. Taskin, M. Kence, and B. Göçmen, “Determination of malathion and diazinon resistance by sequencing the Md alpha E7 gene from Guatemala, Colombia, Manhattan, and Thailand housefly (Musca domestica L.) strains,” RUSSIAN JOURNAL OF GENETICS, pp. 377–380, 2004, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37927.