The genetic basis of malathion resistance in housefly (Musca domestica L.) strains from Turkey

Taskin, V
Kence, M
Organophosphate insecticide (parathion/diazinon) resistance in housefly ( Musca domestica L.) is associated with the change in carboxylesterase activity. The product of MdalphaE7 gene is probably playing a role in detoxification of xenobiotic esters. In our research, we have isolated, cloned and sequenced the MdalphaE7 gene from five different Turkish housefly strains. High doses of malathion ( 600 mug/fly) were applied in a laboratory environment for one year to Ceyhan1, Ceyhan2, Adana, and Ankara strains while no insecticide treatment was performed in the laboratory to Kirazli strain. Trp(251) --> Ser substitution was found in the product of MdalphaE7 gene in all malathion-resistant and Kirazli stocks. In addition, we checked the malathion carboxylesterase (MCE), percent remaining activities in acetylcholinesterase (AChE), glutathion-S-transferase (GST), and general esterase activities in all five strains used in this study. In comparing with universal standard sensitive control WHO, a high level of MCE and GST activities were observed while lower level of general esterase activities was detected in the tested strains. In addition, a higher percent remaining activities in AChE than WHO susceptible strain were observed in all malathion-resistant strains.


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Determination of malathion and diazinon resistance by sequencing the Md alpha E7 gene from Guatemala, Colombia, Manhattan, and Thailand housefly (Musca domestica L.) strains
Taskin, V; Kence, M; Göçmen, Birşen (2004-04-01)
Organophosphate (OP) insecticides (parathion/diazinon) resistance in housefly (Musca domestica L.) is associated with the change in carboxylesterase activity. The product of alphaE7 gene, which is a member of alpha-esterase gene cluster, is probably playing a role in detoxyfication of the xenobiotic esters. In parathion/diazinon resistant M. domestica species Gly(137) to Asp substitution was found in the active center of the product of alphaE7 gene. In malathion (an OP) resistant M. domestica strains Trp(25...
Citation Formats
V. Taskin and M. Kence, “The genetic basis of malathion resistance in housefly (Musca domestica L.) strains from Turkey,” RUSSIAN JOURNAL OF GENETICS, pp. 1215–1222, 2004, Accessed: 00, 2020. [Online]. Available: