FTIR spectroscopy offers hints towards widespread molecular changes in cobalt-acclimated freshwater bacteria

Date

2014-10-01

Author

Kardaş, Mehmet
Gözen, Ayşe Gül
Severcan, Feride

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

140
views

0
downloads

High concentrations of heavy metals can be toxic for bacteria. However, after prolonged exposure, bacteria can become acclimated and begin to be able to grow in the presence of heavy metals. Acclimation can involve alterations of metabolism and molecular structures. Our aim was to examine these alterations in cobalt-acclimated bacteria via attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy on viable samples. Bacillus sp. and Pseudomonas sp. isolated from a temperate shallow lake and a well-established strain of E. coli were investigated. Our results revealed consistent, wide-spread changes in cell membrane and cell wall dynamics of Bacillus sp. and E. coli, including a decrease in peptidoglycan content of Bacillus sp. and increased lipid ordering of the membrane in both bacteria. Furthermore, a decrease in RNA and protein concentrations of Bacillus sp. was measured. All three bacteria studied showed a decrease in conformational freedom of proteins following cobalt acclimation. Interestingly, both Bacillus sp. and E. coli showed slight but significant alterations in their DNA conformations which might imply a methylation-mediated memory formation leading to epigenetic modulation for cobalt adaptation.

Subject Keywords

Aquatic Science, Health, Toxicology and Mutagenesis

URI

https://hdl.handle.net/11511/38533

Journal

AQUATIC TOXICOLOGY

DOI

https://doi.org/10.1016/j.aquatox.2014.05.027

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Effect of acclimatization of microorganisms to heavy metals on the performance of activated sludge process Ozbelge, Tulay A.; Özbelge, Hilmi Önder; Altinten, Pinar (Elsevier BV, 2007-04-02) Although selected heavy metals (HMs) stimulate biological reactions at low concentrations, all HMs are toxic to microorganisms (MOs) at moderate concentrations and can cause inhibitory effects on the biological processes. Therefore, MOs must be acclimated to HMs or other toxic substances present in wastewaters (WWs) before they are used in an activated sludge process (ASP). In this study, combined effect of Cu2+ and Zn2+ ions in a synthetic WW on the efficiency of a laboratory- scale ASP without recycle was...
Diversity of the Aromatic-Ring-Hydroxylating Dioxygenases in the Monoaromatic Hydrocarbon Degraders Held by a Common Ancestor Yavas, Alper; İçgen, Bülent (Springer Science and Business Media LLC, 2018-09-01) Aromatic ring hydroxylating dioxygenases (ARHDs), harboured by a variety of bacteria, catalyze the initial reaction in the degradation of a wide range of toxic environmental contaminants like aromatic and polycyclic aromatic hydrocarbons (PAHs). Regardless of the source, bacteria harbouring RHDs play major role in the removal of these toxic contaminants. The diversity of ARHDs in contaminated sites is supposed to be huge. However, most of the ARHD diversity studies are based on the PAH degraders and the ARH...
Use of cadA-Specific Primers and DNA Probes as Tools to Select Cadmium Biosorbents with Potential in Remediation Strategies İçgen, Bülent (Springer Science and Business Media LLC, 2016-05-01) Biosorption, using cadmium-resistant bacterial isolates, is often regarded as a relatively inexpensive and efficient way of cleaning up wastes, sediments, or soils polluted with cadmium. Therefore, many efforts have been devoted to the isolation of cadmium-resistant isolates for the efficient management of cadmium remediation processes. However, isolation, identification and in situ screening of efficient cadmium-resistant isolates are primary challenges. To overcome these challanges, in this study, cadA, c...
High-rate anaerobic treatment of digestate using fixed film reactors Ülgüdür, Nilüfer; Bayramoğlu, Tuba Hande; Uludağ-Demirer, Sibel; Demirer, Göksel (Elsevier BV, 2019-09-01) The effluent stream of the anaerobic digestion processes, the digestate, accommodates high residual organic content that needs to be further treated before discharge. Anaerobic treatment of digestate would not only reduce the residual organic compounds in digestate but also has a potential to capture the associated biogas. High-rate anaerobic reactor configurations can treat the waste streams using lower hydraulic retention times which requires less footprint opposed to the conventional completely stirred t...
Trace level determination of beryllium in natural and flavored mineral waters after pre-concentration using activated carbon KILINÇ, ERSİN; Bakirdere, Sezgin; YAMAN, MEHMET (Informa UK Limited, 2011-01-01) The concentrations of beryllium (Be) in natural and flavored mineral water samples were determined by flame atomic absorption spectrophotometer (FAAS) after pre-concentration based on the complexation of Be(+2) with a mixture of acetylacetone (pentane-2,4-dione) plus morin (3,5,7,2',4'-pentaoxyflavone) and adsorption on activated carbon. The adsorbed complex was eluted with 1.5 ml of 2.0 M HNO(3) and evaporated to dryness. After adding 1.5 ml of 2 M HNO(3) and centrifuging, Be in acid solution was determine...

Citation Formats

M. Kardaş, A. G. Gözen, and F. Severcan, “FTIR spectroscopy offers hints towards widespread molecular changes in cobalt-acclimated freshwater bacteria,” AQUATIC TOXICOLOGY, pp. 15–23, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/38533.