Salmonella enterica Serotype 4,5,12:i:-, an Emerging Salmonella Serotype That Represents Multiple Distinct Clones

Soyer, Yeşim
Davis, M. A.
Maurer, J.
McDonough, P. L.
Schoonmaker-Bopp, D. J.
Dumas, N. B.
Root, T.
Warnick, L. D.
Groehn, Y. T.
Wiedmann, M.
The prevalence, among human clinical cases, of Salmonella enterica serotype 4,5,12: i:-, a serotype antigenically similar to Salmonella enterica serotype Typhimurium but lacking second-phase flagellar antigens, has increased considerably over the last 10 years. To probe the evolution and ecology of this emerging serotype, we characterized 190 Salmonella isolates initially classified as Salmonella serotypes 4,5,12: i:- (n = 90) and Typhimurium (n = 100) and obtained from various sources in the United States and Spain. These isolates were characterized into six sequence types (determined by multilocus sequence typing [MLST]) and 79 pulsed-field gel electrophoresis types. The majority of Salmonella serotype 4,5,12: i:- and Typhimurium isolates (85 and 84 isolates, respectively) represented a single MLST type. Existing genome information revealed different genome deletions (which included genes responsible for phase 2 flagellum expression) in four Spanish Salmonella serotype 4,5,12: i:- isolates and one U. S. Salmonella serotype 4,5,12: i:- isolate. Fifty-nine isolates of both serotypes, representing different sources and geographical locations as well as different molecular subtypes, were thus screened for the presence of six genes and one specific region, all of which were previously found to show variable presence among Salmonella serotype 4,5,12: i:- and Typhimurium strains. All Salmonella serotype 4,5,12: i:- isolates lacked the phase 2 flagella genes fljA and fljB, which were present in all Salmonella serotype Typhimurium isolates. While all Spanish Salmonella serotype 4,5,12: i:- isolates carried the same deletion surrounding fljAB, all but two U. S. isolates showed a different genomic deletion; the two atypical U. S. isolates represented the "Spanish" deletion genotype and a unique deletion genotype. Salmonella serotype 4,5,12: i:- thus appears to represent at least two common clones, which cannot easily be differentiated with standard diagnostic procedures.


Multilocus Variable-Number Tandem-Repeat Method for Typing Salmonella enterica Serovar Newport
Davis, Margaret A.; Baker, Katherine N. K.; Call, Douglas R.; Warnick, Lorin D.; Soyer, Yeşim; Wiedmann, Martin; Grohn, Yrjo; McDonough, Patrick L.; Hancock, Dale D.; Besser, Thomas E. (American Society for Microbiology, 2009-06-01)
In recent years, the proportion of Salmonella enterica infections represented by S. enterica serovar Newport has increased markedly among humans and animals. Multilocus variable-number tandem-repeat analysis (MLVA) has proven to be useful in discriminating other highly clonal Salmonella serovars. Here, we report on the development of a highly discriminatory MLVA for Salmonella serovar Newport.
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Davis, Margaret A.; Lim, Ji Youn; Soyer, Yeşim; Harbottle, Heather; Chang, Yung-Fu; New, Daniel; Orfe, Lisa H.; Besser, Thomas E.; Call, Douglas R. (Elsevier BV, 2010-07-01)
A microarray was developed to simultaneously screen Escherichia coli and Salmonella enterica for multiple genetic traits. The final array included 203 60-mer oligonucleotide probes, including 117 for resistance genes, 16 for virulence genes, 25 for replicon markers, and 45 other markers. Validity of the array was tested by assessing inter-laboratory agreement among four collaborating groups using a blinded study design. Internal validation indicated that the assay was reliable (area under the receiver-opera...
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İçgen, Bülent; Gültekin, Güzin Candan; Özcengiz, Gülay (Elsevier BV, 2002-01-01)
20 local isolates of enterics belonging to the genera Salmonella, Enterobacter Proteus, Citrobacter from human, chicken and/or egg were characterised for their antibiotic resistance patterns, plasmid profiles, phage types, outer membrane proteins, and lipopolysaccharide patterns. Relatedness of these characteristics for epidemiological analysis was assessed. 18 (90%) strains were resistant to at least one antibiotic and those (multi-drug resistant ones) resisting to two or more antibiotics constituted 50% o...
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The members of the genus Streptomyces are well-known for their capacity to synthesize a vast repertoire of secondary metabolites, including many useful antibiotics and proteins. Streptomyces clavuligerus is the producer of the medically important β-lactam antibiotics such as cephamycin C and the potent β-lactamase inhibitor clavulanic acid. The aspartate pathway of S. clavuligerus is an important primary metabolic pathway providing substrates for β-lactam synthesis. This pathway uses L-aspartic acid as the ...
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Okay, Sezer; Özcengiz, Gülay; Department of Biology (2005)
Chitinases have been shown to be potential agents for biological control of the plant diseases caused by various phytopathogenic fungi and insect pests, because fungal cell walls and insect exoskeletons contain chitin as a major structural component. Chitinase has also been found to increase the efficacy and potency of Bacillus thuringiensis crystal (Cry) proteins toxic to larvae of insect pests. The reason of this synergy is the presence of chitin in the structure of the outer membrane of larval midgut. In...
Citation Formats
Y. Soyer et al., “Salmonella enterica Serotype 4,5,12:i:-, an Emerging Salmonella Serotype That Represents Multiple Distinct Clones,” JOURNAL OF CLINICAL MICROBIOLOGY, pp. 3546–3556, 2009, Accessed: 00, 2020. [Online]. Available: