Development and validation of a resistance and virulence gene microarray targeting Escherichia coli and Salmonella enterica

Download

index.pdf

Date

2010-07-01

Author

Davis, Margaret A.
Lim, Ji Youn
Soyer, Yeşim
Harbottle, Heather
Chang, Yung-Fu
New, Daniel
Orfe, Lisa H.
Besser, Thomas E.
Call, Douglas R.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

149
views

75
downloads

A microarray was developed to simultaneously screen Escherichia coli and Salmonella enterica for multiple genetic traits. The final array included 203 60-mer oligonucleotide probes, including 117 for resistance genes, 16 for virulence genes, 25 for replicon markers, and 45 other markers. Validity of the array was tested by assessing inter-laboratory agreement among four collaborating groups using a blinded study design. Internal validation indicated that the assay was reliable (area under the receiver-operator characteristic curve = 0.97). Inter-laboratory agreement, however, was poor when estimated using the intraclass correlation coefficient, which ranged from 0.27 (95% confidence interval 0.24, 0.29) to 0.29 (0.23, 0.34). These findings suggest that extensive testing and procedure standardization will be needed before bacterial genotyping arrays can be readily shared between laboratories.

Subject Keywords

Microbiology (medical), Molecular Biology, Microbiology

URI

https://hdl.handle.net/11511/38750

Journal

JOURNAL OF MICROBIOLOGICAL METHODS

DOI

https://doi.org/10.1016/j.mimet.2010.03.017

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Investigation of cytocidal effect of K5 type yeast killer protein on sensitive microbial cells Sertkaya, Abdullah; İzgü, Kadri Fatih; Department of Biology (2005) Some yeasts secrete polypeptide toxins, which are lethal to other sensitive yeast cells, gram-positive pathogenic bacteria and pathogenic fungi. Therefore these are designated as killer toxins. Killer toxins are suggested as potent antimicrobial agents especially for the protection of fermentation process against contaminating yeasts, biological control of undesirable yeasts in the preservation of foods. Moreover they are promising antimicrobial agents in the medical field; due to immune system suppressing ...
Fast fluorometric enumeration of E. coli using passive chip KASAP, ESİN NAGİHAN; DOĞAN, ÜZEYİR; ÇOĞUN, FERAH; Yıldırım, Ender; BOYACI, İSMAİL HAKKI; ÇETİN, DEMET; SULUDERE, ZEKİYE; TAMER, UĞUR; ERTAŞ, NUSRET (Elsevier BV, 2019-09-01) In this report, a passive microfluidic chip design was developed for fast and sensitive fluorometric determination of Escherichia coli (E. coli) based on sandwich immunoassay. Initially, magnetic nanoparticles (MNPs) and chitosan modified mercaptopropionic acid capped cadmium telluride (CdTe) quantum dots (QDs) were functionalized with E.coli specific antibody to form a sandwich immunoassay with the E. coli. The magnetic separation and preconcentration of the E.coli from the sample solution was performed in...
Characterisation of local isolates of Enterobacteriaceae from Turkey İçgen, Bülent; Gültekin, Güzin Candan; Özcengiz, Gülay (Elsevier BV, 2002-01-01) 20 local isolates of enterics belonging to the genera Salmonella, Enterobacter Proteus, Citrobacter from human, chicken and/or egg were characterised for their antibiotic resistance patterns, plasmid profiles, phage types, outer membrane proteins, and lipopolysaccharide patterns. Relatedness of these characteristics for epidemiological analysis was assessed. 18 (90%) strains were resistant to at least one antibiotic and those (multi-drug resistant ones) resisting to two or more antibiotics constituted 50% o...
Recombinant plasmid-based quantitative Real-Time PCR analysis of Salmonella enterica serotypes and its application to milk samples Gokduman, Kurtulus; AVŞAROĞLU ERKAN, MÜRŞİDE DİLEK; Cakiris, Aris; Ustek, Duran; Gültekin, Güzin Candan (Elsevier BV, 2016-03-01) The aim of the current study was to develop, a new, rapid, sensitive and quantitative Salmonella detection method using a Real-Time PCR technique based on an inexpensive, easy to produce, convenient and standardized recombinant plasmid positive control. To achieve this, two recombinant plasmids were constructed as reference molecules by cloning the two most commonly used Salmonella-specific target gene regions, invA and ttrRSBC The more rapid detection enabled by the developed method (21 h) compared to the ...
Salmonella enterica Serotype 4,5,12:i:-, an Emerging Salmonella Serotype That Represents Multiple Distinct Clones Soyer, Yeşim; Davis, M. A.; Maurer, J.; McDonough, P. L.; Schoonmaker-Bopp, D. J.; Dumas, N. B.; Root, T.; Warnick, L. D.; Groehn, Y. T.; Wiedmann, M. (American Society for Microbiology, 2009-11-01) The prevalence, among human clinical cases, of Salmonella enterica serotype 4,5,12: i:-, a serotype antigenically similar to Salmonella enterica serotype Typhimurium but lacking second-phase flagellar antigens, has increased considerably over the last 10 years. To probe the evolution and ecology of this emerging serotype, we characterized 190 Salmonella isolates initially classified as Salmonella serotypes 4,5,12: i:- (n = 90) and Typhimurium (n = 100) and obtained from various sources in the United States ...

Citation Formats

M. A. Davis et al., “Development and validation of a resistance and virulence gene microarray targeting Escherichia coli and Salmonella enterica,” JOURNAL OF MICROBIOLOGICAL METHODS, pp. 36–41, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/38750.