Design of a biosensor based on 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H pyrrole

Date

2010-07-01

Author

Tuncagil, Sevinc
Varis, Serhat
Toppare, Levent Kamil

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

138
views

0
downloads

Immobilization of polyphenol oxidase (tyrosinase, E.C. 1.14.18.1) was achieved on a copolymer of 1-(4-nitrophenyl)-2,5-di(2-thieny1)-1H-pyrrole [SNS(NO2)] with pyrrole USNS(NO2)]/PPy) via electrochemical polymerization. Two different substrates; catechol and L-tyrosine were used for the characterization of biosensor. The kinetic parameters of the biosensor, maximum reaction rate of the enzyme (V-max) and Michaelis-Menten constant (K-m) were determined for two different substrates. V-max was found as 0.02 mu mol/min electrode for both substrates. K-m values were determined as 250 and 2 mM for catechol and L-tyrosine respectively. Calibration curves for enzyme activity versus substrate concentration were plotted between 0.05 and 0.5 M catechol and between 0.8 and 2.5 mM L-tyrosine. Optimum temperature and pH, operational and storage stabilities of immobilized enzyme were examined. (C) 2009 Elsevier B.V. All rights reserved,

Subject Keywords

Conducting polymers, Electrochemical biosensors, Enzyme immobilization, Catechol, L-Tyrosine, Tyrosinase

URI

https://hdl.handle.net/11511/39517

Journal

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

DOI

https://doi.org/10.1016/j.molcatb.2009.06.002

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Preparation of biosensors by immobilization of polyphenol oxidase in conducting copolymers and their use in determination of phenolic compounds in red wine Boyukbayram, A. Efif; Kiralp, Senem; Toppare, Levent Kamil; Yagci, Yusuf (2006-10-01) Electrochemically produced graft copolymers of thiophene capped polytetrahydofuran (TPTHF1 and TPTHF2) and pyrrole were achieved by constant potential electrolysis using sodium dodecylsulfate (SDS) as the supporting electrolyte. Characterizations were based on Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Electrical conductivities were measured by the four-probe technique.
Synthesis of conducting block copolymers and their use in the immobilization of invertase and polyphenol oxidase enzyme Kıralp, Senem; Kıralp, Senem; Department of Chemistry (2004) A new thiophene derivative containing menthyl group (MM) was synthesized and polymerized via chemical and electrochemical methods. Polymers obtained and MM itself were used to synthesize copolymers with pyrrole under conditions of constant potential electrolysis. Cyclic Voltammetry, thermal analysis and scanning electron microscopy analyses were performed for the characterization of samples. Immobilization of invertase and polyphenol oxidase enzymes was performed in the matrices obtained via copolymerizatio...
Characterization of conducting copolymer of pyrrole via pyrolysis mass spectrometry Levent, Anil; Hacaloğlu, Jale; Toppare, Levent Kamil (2008-03-01) In this work, structural and thermal characterization of BF4- doped copolymer of pyrrole (PPy) with 2-methylbutyl-2-(3-thienyl)acetate prepared by electrochemical polymerization were performed via a pyrolysis mass spectrometry technique. The pyrolysis mass spectrometry data of the copolymer PPy/PMBTA, and the homopolymers; polypyrrole, PPy, and poly(2-methylbutyl-2-(3-thienyl)acetate), PMBTA were analyzed and compared. It has been determined that when the electrochemical polymerization of pyrrole was achiev...
Characterization of conducting copolymers of succinic acid bis-(-4-pyrrol-1-yl-phenyl) ester and thiophene via pyrolysis mass spectrometry Ertas, Merve; Hacaloğlu, Jale; Toppare, Levent Kamil (2005-03-01) In this work, a direct pyrolysis mass spectrometry technique was applied to characterize a conducting copolymer of succinic acid bis-(4-pyrrol-1-yl-phenyl) ester (SM) and thiophene prepared by electrochemical polymerization. Thermal degradation behavior of both components was quite similar to those of the corresponding homopolymers, PSM, and polythiophene PTh. Yet, detection of oligomers and mixed dimers of both monomers in the high temperature pyrolysis mass spectra confirmed the formation of a copolymer. ...
Synthesis and electro-optical properties of new conjugated hybrid polymers based on furan and fluorene units Gunes, Arzu; CİHANER, ATİLLA; Önal, Ahmet Muhtar (2013-02-01) A novel series of hybrid monomers containing furan and fluorene units, namely 2-(2-(furan-2-yl)-9H-fluoren-7-yl)furan (FFF), 2,7-di(furan-2-yl)-9H-fluoren-9-one (FOF) and 2-(2-(furan-2-yl)-9,9-dihexyl-9H-fluoren-7-yl)furan (FHF), was synthesized and their electrochemical polymerization was achieved via potential cycling. Optical and electrochemical properties of the polymers, poly(2-(2-(furan-2-yl)9H-fluoren-7-yl)furan (PFFF), poly(2,7-di(furan-2-yl)-9H-fluoren-9-one) (PFOF) and poly(2-(2-(furan2-yl)-9,9-di...

Citation Formats

S. Tuncagil, S. Varis, and L. K. Toppare, “Design of a biosensor based on 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H pyrrole,” JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, pp. 195–199, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39517.