Preparation of biosensors by immobilization of polyphenol oxidase in conducting copolymers and their use in determination of phenolic compounds in red wine

Boyukbayram, A. Efif
Kiralp, Senem
Toppare, Levent Kamil
Yagci, Yusuf
Electrochemically produced graft copolymers of thiophene capped polytetrahydofuran (TPTHF1 and TPTHF2) and pyrrole were achieved by constant potential electrolysis using sodium dodecylsulfate (SDS) as the supporting electrolyte. Characterizations were based on Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Electrical conductivities were measured by the four-probe technique.


Production and biochemical characterization of polyphenol oxidase from thermomyces lanuginosus
Astarcı, Erhan; Bakır, Ufuk; Ögel, Zümrüt B.; Department of Biotechnology (2003)
Polyphenol oxidases are enzymes that catalyze the oxidation of certain phenolic substrates to quinones in the presence of molecular oxygen. Polyphenol oxidases are widely used in several applications. In food industry, they are used for enhancement of flavor in coffee, tea and cocoa production, and determination of food quality. In medicine, they have several uses in treatments of Parkinson̕s disease, phenlyketonurea and leukemia. In wastewater treatment, they are used for the removal of phenolic pollutants...
Synthesis of conducting block copolymers and their use in the immobilization of invertase and polyphenol oxidase enzyme
Kıralp, Senem; Kıralp, Senem; Department of Chemistry (2004)
A new thiophene derivative containing menthyl group (MM) was synthesized and polymerized via chemical and electrochemical methods. Polymers obtained and MM itself were used to synthesize copolymers with pyrrole under conditions of constant potential electrolysis. Cyclic Voltammetry, thermal analysis and scanning electron microscopy analyses were performed for the characterization of samples. Immobilization of invertase and polyphenol oxidase enzymes was performed in the matrices obtained via copolymerizatio...
Immobilization of invertase in conducting copolymers of 3-methylthienyl methacrylate
Çırpan, Ali; Toppare, Levent Kamil; Yagci, Y. (Elsevier BV, 2003-4)
Immobilization of invertase in conducting copolymer matrices of 3-methylthienyl methacrylate with pyrrole and thiophene was achieved by constant potential electrolysis using sodium dodecyl sulfate (SDS) as the supporting electrolyte. Polythiophene (PTh) was also used in entrapment process for comparison. Kinetic parameters, Michaelis-Menten constant, Km, and the maximum reaction rate, Vmax, were investigated. Operational stability and temperature optimization of the enzyme electrodes were also examined.
Characterization of conducting copolymer of pyrrole via pyrolysis mass spectrometry
Levent, Anil; Hacaloğlu, Jale; Toppare, Levent Kamil (2008-03-01)
In this work, structural and thermal characterization of BF4- doped copolymer of pyrrole (PPy) with 2-methylbutyl-2-(3-thienyl)acetate prepared by electrochemical polymerization were performed via a pyrolysis mass spectrometry technique. The pyrolysis mass spectrometry data of the copolymer PPy/PMBTA, and the homopolymers; polypyrrole, PPy, and poly(2-methylbutyl-2-(3-thienyl)acetate), PMBTA were analyzed and compared. It has been determined that when the electrochemical polymerization of pyrrole was achiev...
Synthesis and characterization of poly(oxalic acid dithiophen-3-yl methyl ester) and thiophene ended poly-caprolactone
Kerman, İpek; Toppare, Levent Kamil; Department of Chemistry (2004)
Synthesis and characterization of thiophene ended poly-e-caprolactone (PCL) and oxalic acid dithiophen-3-yl methyl ester (ODME) and their copolymers with both pyrrole and thiophene were achieved. Chemical structure of the precursor polymer and monomer were investigated Redox behavior of polymer and monomers were determined by Cyclic Voltammetry (CV). Structural characterization of samples were carried out by 1H, 13C Nuclear Magnetic Resonance (NMR) and Fourier Transform Infrared Spectroscopy (FTIR). Conduct...
Citation Formats
A. E. Boyukbayram, S. Kiralp, L. K. Toppare, and Y. Yagci, “Preparation of biosensors by immobilization of polyphenol oxidase in conducting copolymers and their use in determination of phenolic compounds in red wine,” BIOELECTROCHEMISTRY, pp. 164–171, 2006, Accessed: 00, 2020. [Online]. Available: