Genetic screening of Turkish wheat varieties for the durable resistance gene, Lr34

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2011

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Boylu, Barış

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Wheat diseases such as rusts and powdery mildews are among the most important and ancient diseases that affect wheat cultivation worldwide. The pathogen race specific resistance genes cannot maintain long lasting resistance. On the other hand, the presence of genes confers the non-race specific resistance last much longer. The durable resistance phenotypes in wheat against various rust and powdery mildew diseases were reported as Lr34, Yr18, and Pm38 separately; nevertheless, they were known to locate very close to each other based on linkage analysis. Recently, it was shown that all of these resistance phenotypes are indeed conferred by the same gene, encoding Adenosine triphosphate–binding cassette transporter (ABC-transporter) type protein. The way with which this transporter is functioning to maintain the durable resistance against different types of pathogens is still unknown. v The presence of LR34 (or ABC-transporter) gene resistant allele can be detected by specially designed markers. In spite of the few DNA sequence differences between the resistant and the susceptible alleles, the easily applicable PCR based markers allow the detection of the presence of this durable resistant allele on any given variety or cultivar. In our study, 62 different Turkish bread wheat varieties were screened by the gene specific molecular markers, developed from those LR34 gene mutation sites. The 11 cultivars determined to contain the resistant allele. This study is the first screening of Turkish cultivars for the presence of LR34 gene.

Subject Keywords

Biotechnology, Wheat

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http://etd.lib.metu.edu.tr/upload/12613140/index.pdf
https://hdl.handle.net/11511/20551

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

B. Boylu, “Genetic screening of Turkish wheat varieties for the durable resistance gene, Lr34,” M.S. - Master of Science, Middle East Technical University, 2011.