Genetic screening of Turkish wheat varieties for the durable resistance gene, Lr34

Download

index.pdf

Date

2011

Author

Boylu, Barış

Metadata

Show full item record

Item Usage Stats

405
views

300
downloads

Wheat diseases such as rusts and powdery mildews are among the most important and ancient diseases that affect wheat cultivation worldwide. The pathogen race specific resistance genes cannot maintain long lasting resistance. On the other hand, the presence of genes confers the non-race specific resistance last much longer. The durable resistance phenotypes in wheat against various rust and powdery mildew diseases were reported as Lr34, Yr18, and Pm38 separately; nevertheless, they were known to locate very close to each other based on linkage analysis. Recently, it was shown that all of these resistance phenotypes are indeed conferred by the same gene, encoding Adenosine triphosphate–binding cassette transporter (ABC-transporter) type protein. The way with which this transporter is functioning to maintain the durable resistance against different types of pathogens is still unknown. v The presence of LR34 (or ABC-transporter) gene resistant allele can be detected by specially designed markers. In spite of the few DNA sequence differences between the resistant and the susceptible alleles, the easily applicable PCR based markers allow the detection of the presence of this durable resistant allele on any given variety or cultivar. In our study, 62 different Turkish bread wheat varieties were screened by the gene specific molecular markers, developed from those LR34 gene mutation sites. The 11 cultivars determined to contain the resistant allele. This study is the first screening of Turkish cultivars for the presence of LR34 gene.

Subject Keywords

Biotechnology, Wheat

URI

http://etd.lib.metu.edu.tr/upload/12613140/index.pdf
https://hdl.handle.net/11511/20551

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Investigating the roles of micrornas in biotic stress responses and functional characterization of a novel ztl-type f-box protein via virus induced gene silencing Dağdaş, Yasin Fatih; Akkaya, Mahinur S.; Department of Biotechnology (2009) Barley and wheat are the two most important crop species in Turkey. Molecular studies for increasing crop yield of these species are very important for the economic benefits of Turkey. Powdery mildew and yellow rust are the two main pathogens, infecting barley and wheat, respectively in our country and causing a great amount of yield loss each year. Till now, classical genetics studies were performed in order to develop resistant barley and wheat cultivars, but these studies have not been succesful. Therefo...
Functional analysis of a mirna putatively involved in powdery mildew disease susceptibility in barley Dağdaş, Gülay; Akkaya, Mahinur S.; Department of Biotechnology (2009) Barley is one of the most important crop species in Turkey and powdery mildew is one of the most common pathogen decreasing yield in barley. For this problem, agricultural biologists apply breeding technologies in order to select and propagate resistant barley cultivars. However, this is not a permanent solution since pathogens evolve rapidly to overcome plant resistance mechanisms. On the other hand, molecular plant pathologists are trying to understand basic mechanisms underlying plant-pathogen interactio...
Expression analysis of NAC type transcription factors on wheat seedlings under abiotic stress conditions Baloğlu, Mehmet Cengiz; Yücel, Ayşe Meral; Öktem, Hüseyin Avni; Department of Biology (2011) Wheat is the most important grain crop grown in our country providing greatest part of the daily nutritional requirement. Abiotic factors including salinity, drought, cold and heat stresses affect quality and yield of wheat varieties used for the production of both bread and pasta flour. NAC proteins form one of the widest families of plant specific transcription factors. Members of this family are related with development, defense and abiotic stress responses. TaNAC69-1 and TtNAM-B2 genes were isolated fro...
Bacterial expression of an effector protein of yellow rust pathogen and a resistance protein of wheat and characterization of the effector protein Erdoğan, Sayıt Mahmut; Akkaya, Mahinur S.; Özçubukçu, Salih; Department of Biotechnology (2019) Yellow rust is one of the most important wheat disease encountered in large parts of the world and in our country caused by a pathogen which is called as Puccinia striiformis f. sp. tritici (Pst). Rapid alterations in the pathogen virulency can make previously resistant varieties susceptible to the disease. Finding out common or species-specific genes, participated in the plant-pathogen interactions will provide an understanding of the biological mechanisms of the disease. Within the scope of this thesis fo...
Biodegradation of the Allelopathic Chemical Pterostilbene by a Sphingobium sp. Strain from the Peanut Rhizosphere Yu, Ri-Qing; Kurt, Zöhre; He, Fei; Spain, Jim C. (American Society for Microbiology, 2019-03-01) Many plants produce allelopathic chemicals, such as stilbenes, to inhibit pathogenic fungi. The degradation of allelopathic compounds by bacteria associated with the plants would limit their effectiveness, but little is known about the extent of biodegradation or the bacteria involved. Screening of tissues and rhizosphere of peanut (Arachis hypogaea) plants revealed substantial enrichment of bacteria able to grow on resveratrol and pterostilbene, the most common stilbenes produced by the plants. Investigati...

Citation Formats

B. Boylu, “Genetic screening of Turkish wheat varieties for the durable resistance gene, Lr34,” M.S. - Master of Science, Middle East Technical University, 2011.