Fed-Batch Biomolecule Production by Bacillus subtilis: A State of the Art Review

Date

2016-04-01

Author

Ozturk, Sibel
Çalık, Pınar
Ozdamar, Tuncer H.

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Bacillus subtilis is a highly promising production system for various biomolecules. This review begins with the algorithm of fed-batch operations (FBOs) and then illustrates the approaches to design the initial production medium and/or feed stream. Additionally, the feeding strategies developed with or without feedback control for fed-batch B. subtilis fermentations were compiled with a special emphasis on recombinant protein (r-protein) production. For biomolecule production by wild-type B. subtilis, due to the different intracellular production patterns, no consensus exists on the FBO strategy that gives the maximum productivity, whereas for r-protein production appropriate feeding strategies vary depending on the promoter used. Thus, we conclude that the B. subtilis community is still seeking an approved strong promoter and generalized FBO strategies.

Subject Keywords

Biotechnology, Bioengineering

URI

https://hdl.handle.net/11511/39677

Journal

TRENDS IN BIOTECHNOLOGY

DOI

https://doi.org/10.1016/j.tibtech.2015.12.008

Collections

Department of Chemical Engineering, Article

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Citation Formats

S. Ozturk, P. Çalık, and T. H. Ozdamar, “Fed-Batch Biomolecule Production by Bacillus subtilis: A State of the Art Review,” TRENDS IN BIOTECHNOLOGY, pp. 329–345, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39677.