Obtaining durable enzyme powder via spray drying

Namaldı, Ayşegül
Serine alkaline protease (SAP, EC produced by Bacillus species, that are the microbioreactors within the bioreactors, is one of the major industrial enzymes. In this study, after production by Recombinant Bacillus subtilis (BGSC-1A751), carrying pHV1431::subc gene in the complex media and separation of solids, SAP was dried by using a spray drier. Experiments were performed to investigate the stabilization of SAP during spray drying and subsequent storage. Initially, the effect of air inlet temperature of the spray drier on SAP activity was evaluated. For this purpose, SAP solutions were spray dried in the absence of any protective agents at five different air inlet temperature (700C, 900C, 1100C, 1200C, 1300C). As a result, increasing air inlet temperature lead to an increase in activity loss of SAP during drying. Thereafter, the effect of protective additives, glucose and maltodextrin (0.5%, 1%, 2% w/v), on SAP activity was investigated during spray drying. The activity loss of SAP was completely inhibited in the presence of glucose at 70, 90 and 1100C. However, the addition of maltodextrin was better than glucose for activity preservation of SAP at 1300C. Among the obtained results, 1% glucose addition was the best to preserve activity of SAP during spray drying. Then, structural change of SAP during drying was investigated. FTIR-ATR spectrum was used to evaluate the change in physical structure of the dried SAP powders in the presence of 1% glucose. From infrared images, at 900C more native-like structure for dried SAP powders was observed. In the last stage of this study storage stability of obtained SAP powders at 40C for a long period (6 months) was investigated. When considering all conditions, 0.5% maltodextrin addition was the best for stabilizing SAP powders along storage time.


Fed-Batch Biomolecule Production by Bacillus subtilis: A State of the Art Review
Ozturk, Sibel; Çalık, Pınar; Ozdamar, Tuncer H. (Elsevier BV, 2016-04-01)
Bacillus subtilis is a highly promising production system for various biomolecules. This review begins with the algorithm of fed-batch operations (FBOs) and then illustrates the approaches to design the initial production medium and/or feed stream. Additionally, the feeding strategies developed with or without feedback control for fed-batch B. subtilis fermentations were compiled with a special emphasis on recombinant protein (r-protein) production. For biomolecule production by wild-type B. subtilis, due t...
Effects of bioreactor operation parameters on intracellular reaction rate distribution in beta-lactamase production by bacillus species
Arifoğlu, Müge; Çalık, Pınar; Department of Chemical Engineering (2004)
In this study, the effects of oxygen transfer (OT) on beta-lactamase production and on intracellular reaction rates were investigated with Bacillus licheniformis ATCC 2597. In order to clarify the oxygen transfer effects on the production of beta-lactamase, firstly a glucose based defined medium was designed and using this medium, the effects of bioreactor operation parameters, i.e., pH and temperature, on beta-lactamase activity and cell formation were investigated in laboratory scale batch-bioreactors usi...
Studies on selective adsorption of aqueous glucose or fructose on various cationic forms of Zeolite Y
Yeşiltepe, Suat Bora; Kıncal, N. Suzan; Department of Biotechnology (2006)
The equilibria of adsorption on calcium and hydrogen forms of zeolite Y by equimolar solutions of 12.5 %, 20%, 25%, 30% and 35% w/v of mixtures of glucose, G and fructose, F; also the non-equimolar mixtures of 20% w/v glucose - 30% w/v fructose, 30% w/v glucose - 20% w/v fructose, 25% w/v glucose 35% w/v fructose, and 35% w/v glucose-25% w/v fructose solutions, which were prepared 24 hours in advance at the experimental temperature, have been studied batch wise at 50ºC. Glucose adsorption, in solutions tha...
Preparation of cross-linked tyrosinase aggregates
Aytar, Burcu Selin; Bakir, Ufuk (Elsevier BV, 2008-02-01)
Tyrosinase from mushroom was immobilized as a cross-linked enzyme aggregate (CLEA) via precipitation with ammonium sulfate and cross-linking with glutaraldehyde. The effects of precipitation and cross-linking on CLEA activity were investigated and the immobilized tyrosinase was characterized. Sixty percent ammonium sulfate saturation and 2% glutaraldehyde were used; a 3-h cross-linking reaction at room temperature, at pH 7.0 was performed; particle sizes of the aggregates were reduced; consequently, 100% ac...
In planta determination of GaMyb transcription factor as a target of pathogen induced microRNA, mir159
Akkaya, Mahinur; Dagdas, Gulay Gok; Dagdas, Yasin F. (2011-09-01)
One of the molecular mechanisms underlying the plant–pathogen interactions is the regulation of gene expressions in plant responses by microRNAs which are either stimulated or silenced against pathogen attacks. Among the plant miRNAs, we found that mir159 is one of which that differentially expressed upon Blumeria graminis f. sp. hordei (Bgh) infected resistant and susceptible barley lines. The study aims to confirm its role in regulating one of its putative target genes, GaMyb transcription factor, in vivo...
Citation Formats
A. Namaldı, “Obtaining durable enzyme powder via spray drying,” M.S. - Master of Science, Middle East Technical University, 2005.