Eriochrome Black T-Eu3+ Complex as a Ratiometric Colorimetric and Fluorescent Probe for the Detection of Dipicolinic Acid, a Biomarker of Bacterial Spores

Date

2018-03-20

Author

Yilmaz, M. Deniz
Öktem, Hüseyin Avni

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A novel ratiometric colorimetric and fluorescent dual probe based on Eriochrome Black T (EBT) Eu3+ complex was designed to detect dipicolinic acid (DPA), a major constituent of bacterial spores, with high sensitivity and selectivity. UV vis titration experiments demonstrated that EBT and Eu3+ ions formed a 1:1 coordination pair in water. In the presence of Eu3+ ions, the blue solution of EBT changed to magenta, however, upon the addition of DPA, the magenta color changed to blue immediately and characteristic fluorescence emission from DPA-Eu3+ complex was observed. In addition, the sensitivity of the system was further evaluated on Geobacillus stearothermophilus spores and as low as 2.5 X 10(5) spores were detected.

Subject Keywords

Analytical Chemistry

URI

https://hdl.handle.net/11511/40344

Journal

ANALYTICAL CHEMISTRY

DOI

https://doi.org/10.1021/acs.analchem.8b00576

Collections

Department of Biology, Article

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Citation Formats

M. D. Yilmaz and H. A. Öktem, “Eriochrome Black T-Eu3+ Complex as a Ratiometric Colorimetric and Fluorescent Probe for the Detection of Dipicolinic Acid, a Biomarker of Bacterial Spores,” ANALYTICAL CHEMISTRY, pp. 4221–4225, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40344.