Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Secondary structure and conformational change of mushroom polyphenol oxidase during thermosonication treatment by using FTIR spectroscopy
Date
2017-01-01
Author
Baltacıoğlu, Hande
Bayındırlı, Alev
Severcan, Feride
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
175
views
0
downloads
Cite This
To understand the conformational changes of mushroom PPO, the secondary structural change of the enzyme during thermosonication treatment at different power (60, 80 and 100%), temperature (20-60 degrees C) and time (0-30 min) combinations was investigated by using FTIR spectroscopy and compared with the change in enzyme activity. The enzyme inactivation higher than 99% was obtained at 100% amplitude at 60 degrees C for 10 min. FTIR studies showed that marked spectral changes were noted after ultrasound treatment at 20 degrees C. The alpha-helix and beta-sheet contents decreased, while aggregated beta p-sheet, turns and random coil contents increased as temperature increased up to 60 degrees C during thermosonication treatment for 10 min indicating protein denaturation. Aggregated bands located at 1683 and 1616 cm(-1) became evident after ultrasound treatment at 40 degrees C. When temperature was lowered back to 25 degrees C, from ultrasound treatment at 60 degrees C, these bands were still observed, indicating the irreversible change in the structure.
Subject Keywords
Conformation change
,
Protein secondary structure
,
Spectroscopy
,
FTIR
,
Thermosonication
,
PPO
URI
https://hdl.handle.net/11511/41894
Journal
FOOD CHEMISTRY
DOI
https://doi.org/10.1016/j.foodchem.2016.07.021
Collections
Department of Food Engineering, Article
Suggestions
OpenMETU
Core
Effect of thermal treatment on secondary structure and conformational change of mushroom polyphenol oxidase (PPO) as food quality related enzyme: A FTIR study
Baltacioglu, Hande; Bayındırlı, Alev; Severcan, Mete; Severcan, Feride (2015-11-15)
In order to understand the conformational changes of polyphenol oxidase (PPO), which is a food quality related enzyme, after thermal treatment, secondary structure changes of the enzyme were analyzed by using Fourier Transform Infrared (FTIR) spectroscopy and compared with the change in enzyme activity in the temperature range of 25-80 degrees C. Fourier self-deconvolution, neural network (NN) and curve-fitting analysis were applied to the amide I band of FTIR spectra for detail analysis of secondary struct...
Effects of the non-steroidal anti-inflammatory drug celecoxib on cholesterol containing distearoyl phosphatidylcholine membranes
Sade, Asli; Banerjee, Sreeparna; Severcan, Feride (2011-01-01)
The effects of different concentrations of celecoxib on the acyl chain order, dynamics and the hydration status of the head group and interfacial region of model membranes containing DSPC and cholesterol were investigated in detail using Fourier transform infrared spectroscopy. Our results reveal that regardless of the presence of cholesterol, celecoxib is able to alter the physical properties of membranes. It exerts opposing effects on membrane order at high and low concentrations and decreases membrane fl...
Competitive effect of vitamin D-2 and Ca2+ on phospholipid model membranes: an FTIR study
Toyran, N; Severcan, Feride (2003-04-01)
The interaction of Ca2+, with dipalmitoyl phosphatidylcholine (DPPC) model membranes was studied in the presence and absence of vitamin D-2 by using Fourier transform infrared spectroscopy. Addition of vitamin D-2 and/or Ca2+ into pure DPPC liposomes shifts the phase transition to higher temperature, orders and decreases the dynamics of the acyl chains in both phases and does not induce hydrogen bond formation in the interfacial region. Moreover, the dynamics of the head group of the phospholipid decreases ...
Temperature dependence of the phospholipids bilayers stability, studied by FTIR spectroscopy
Severcan, Feride; Agheorghiesei, Catalin; Dorohoi, Dana-Ortansa (2008-03-01)
The temperature induced modifications in the lipid bilayers in water were studied by using 2D-FTIR Correlation Spectroscopy. The spectral range analysed in this paper corresponds to symmetric and asymmetric stretching vibrations of the -CH2 groups from the acyl chains of DPPC. Autocorrelation peaks at 2916 cm(-1) and 2848.5 cm(-1) and asynchrone modifications in the spectral ranges 2916-3000 cm(-1) and 2848-3000 cm(-1) were evidenced. The revealed modifications indicate changes in the conformers of the hydr...
Physiological changes of Escherichia coli O157:H7 and Staphylococcus aureus following exposure to high hydrostatic pressure
ÇELİK, MUTLU; Yousef, Ahmed; Alpas, Hami (2013-09-01)
Morphological changes and membrane integrity of Escherichia coli O157:H7 and Staphylococcus aureus cells before and after high hydrostatic pressure (HHP) treatments (200-400 MPa) and time (1-5 min), at a constant temperature (40 A degrees C), in peptone water were examined by using scanning electron microscopy (SEM) and fluorescent microscopy, respectively. SEM images showed that unpressurized cells exhibited a smooth surface appearance. E. coli O157:H7 cells exposed to pressure treatments first appeared la...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
H. Baltacıoğlu, A. Bayındırlı, and F. Severcan, “Secondary structure and conformational change of mushroom polyphenol oxidase during thermosonication treatment by using FTIR spectroscopy,”
FOOD CHEMISTRY
, pp. 507–514, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41894.