Effects of the non-steroidal anti-inflammatory drug celecoxib on cholesterol containing distearoyl phosphatidylcholine membranes

Download

10.1155-2011-813853.pdf

Date

2011-01-01

Author

Sade, Asli
Banerjee, Sreeparna
Severcan, Feride

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

200
views

104
downloads

The effects of different concentrations of celecoxib on the acyl chain order, dynamics and the hydration status of the head group and interfacial region of model membranes containing DSPC and cholesterol were investigated in detail using Fourier transform infrared spectroscopy. Our results reveal that regardless of the presence of cholesterol, celecoxib is able to alter the physical properties of membranes. It exerts opposing effects on membrane order at high and low concentrations and decreases membrane fluidity in the presence of cholesterol. An evidence of phase separation has also been observed. The decrease in membrane fluidity supports the hypothesis that celecoxib may induce changes in the activity of membrane bound enzymes through modulating physical properties of the membrane thereby contributing to its anticancer activity. A possible change in the location of celecoxib in DSPC membranes when cholesterol is present has also been proposed. These results clarify, to a certain extent, the molecular interactions of celecoxib with membrane systems and may additionally contribute to a better understanding of COX-2 independent mechanisms of celecoxib action.

Subject Keywords

Celecoxib, Cholesterol, DSPC, FTIR, MLV, Lipid Organization, Model membranes, Vitamin D-2, Fluidity, Spectroscopy, Inhibitors

URI

https://hdl.handle.net/11511/39711

Journal

SPECTROSCOPY-BIOMEDICAL APPLICATIONS

DOI

https://doi.org/ 10.1155/2011/813853

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Effect of thermal treatment on secondary structure and conformational change of mushroom polyphenol oxidase (PPO) as food quality related enzyme: A FTIR study Baltacioglu, Hande; Bayındırlı, Alev; Severcan, Mete; Severcan, Feride (2015-11-15) In order to understand the conformational changes of polyphenol oxidase (PPO), which is a food quality related enzyme, after thermal treatment, secondary structure changes of the enzyme were analyzed by using Fourier Transform Infrared (FTIR) spectroscopy and compared with the change in enzyme activity in the temperature range of 25-80 degrees C. Fourier self-deconvolution, neural network (NN) and curve-fitting analysis were applied to the amide I band of FTIR spectra for detail analysis of secondary struct...
The influence of gramicidin S on the thermotropic phase transition of dipalmitoylphosphatidylglycerol DPPG/GS systems: Experiment and computer model Stan, C; Cristescu, CP; Severcan, Feride; Dorohoi, D (2004-09-01) The paper presents experimental data recorded by Fourier Tran form Infrared Spectroscopy regarding the influence of the antimicrobial peptide gramicidin (GS) concentration on the thermotropic phase transition of dipalmitoylphosphatidylglycerol (DPPG) lipid bilayer membrane in DPPG/GS systems, as reflected in the change of the bandwidth of the symmetric stretching band of CH2. The samples were investigated cyclically in a temperature range between 26.5degrees and 61.1degreesC which includes the gel to liquid...
Concentration dependent different action of progesterone on the order, dynamics and hydration states of the head group of dipalmitoyl-phosphatidylcholine membrane Korkmaz, Filiz; Kirbiyik, Halil; Severcan, Feride (Hindawi Limited, 2005) Interactions of progesterone with zwitterionic dipalmitoyl phosphatidylcholine (DPPC) multilamellar liposomes (MLVs) were investigated as a function of progesterone concentration at selected temperatures monitoring both the gel and liquid crystalline phase, by using Fourier Transform Infrared spectroscopy (FTIR). It has been show that the effect of progesterone on membrane dynamics is dependent on progesterone concentration. At 1 mol%, which is close to physiological level, progesterone behaves differently....
Biophysical studies of progesterone-model membrane interactions Korkmaz, Filiz; Kırbıyık, Halil; Severcan, Feride; Department of Physics (2003) Interactions of progesterone with zwitterionic dipalmitoyl phosphotidylcholine (DPPC) multilamellar liposomes (MLVs) were investigated as a function of temperature and progesterone concentration by using three non-invasive techniques of Fourier transform infrared (FTIR) spectroscopy, turbidity at 440 nm and differential scanning calorimetry (DSC).The results show that lmol% of progesterone does not induce a significant change in the shape of thermotropic profile of DPPC. However as progesterone concentratio...
Effect of gramicidin S on the dipalmitoylphosphatidyl-glycerol thermotropic phase transition in DPPG/GS systems: A mathematical approach Stan, Cristina; Cristescu, C. P.; Severcan, Feride; Dorohoi, Dana (Informa UK Limited, 2006-01-01) We present a mathematical approach to the experimental data recorded via Fourier transform-infrared spectroscopy regarding the influence of the concentration of the antimicrobial peptide gramicidin S (GS) on the thermotropic phase transition of the dipalmitoylphosphatidyl-glycerol (DPPG) lipid bilayer membrane in DPPG/GS systems. The model is based on the influence of the GS concentration on the parameters of a nonlinear damped oscillator, which models the CH2 symmetric stretching band.

Citation Formats

A. Sade, S. Banerjee, and F. Severcan, “Effects of the non-steroidal anti-inflammatory drug celecoxib on cholesterol containing distearoyl phosphatidylcholine membranes,” SPECTROSCOPY-BIOMEDICAL APPLICATIONS, pp. 177–185, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39711.