Phylogenetic relationships among native Oxytropis species in Turkey using the trnL intron, trnL-F IGS, and trnV intron cpDNA regions

Tekpinar, Ayten
Kaya, Zeki
We tested the phylogenetic utility of three chloroplast DNA loci, i.e. the trnL((UAA)) intron,trnL((UAA))-F-(GAA) intergenic spacer (IGS), and trnV((UAC)) intron, across thirteen native Oxytropis species. Our objective was to determine whether any of these chloroplast DNA markers could be beneficial to figure out phylogenetic relationships among Oxytropis species. To increase the interspecific sampling, nine sequences of the trnL intron and trnL-F regions were retrieved from GenBank and included in the analyses. No sequence of the trnV intron region was available in the database and so only sequences of the native species were used for the analyses. Phylogenies derived from maximum likelihood and maximum parsimony analyses indicated that the trnL intron and trnV intron regions provided better resolution for relationships among species with respect to the trnL-F region. The highest variable and parsimony informative sites were observed in the trnL intron region, while the lowest sites were seen in the trnV intron. Less variable sites for the trnV intron region were expected since no foreign sequences could be included in the analysis. Oxytropis lazica was phylogenetically separated from native species and clustered with foreign ones when the trnL intron and trnL-F regions were analyzed. The result obtained from the trnV intron region proved that Oxytropis engizekensis Duman and Vural may be a synonym of O. persica Boiss. A previous study that used morphological characters arrived at a similar conclusion.


Phylogenetic relationships of Elymus L. and related genera (Poaceae) based on the nuclear ribosomal internal transcribed spacer sequences
Dizkirici, Ayten; KAYA, ZEKİ; Cabi, Evren; Doğan, Musa (2010-01-01)
To help in the clarification of the taxonomic status of Elymus L and related genera Agropyron Gaertn Leymus Hochst Hordelymus (Jess) Harz and Brachypodium P Beauv, the sequence diversity in the internal transcribed spacer (ITS) region of nuclear ribosomal DNA was studied ITS data of 64 Triticeae accessions including 40 Elymus 12 Agropyron 7 Leymus and 4 Hordelymus and 1 Brachypodium as an out group were analysed The molecular diversity statistics Indicated that the most diverse genus is Elymus among the stu...
Molecular phylogenetic analyses of Juniperus L. species in Turkey and their relations with other junipers based on cpDNA
Güvendiren, Aysun Demet; Kaya, Zeki; Department of Biology (2015)
Evolutionary relationships within and among two sections and three subsections of Juniperus species (Section Juniperus, subsections Juniperus, Oxycedrus and Caryocedrus, Section Sabina) naturally distributed in Turkey were investigated with molecular variations of chloroplast DNA (cpDNA). This study revealed the phylogenetic relation of 66 individuals from 7 native Turkish Juniperus L. species based upon DNA sequence of trnL intron (trnL5’-L3’) , trnL3’- F(GAA) (trnL-F intergenic spacer), trnV intron and ma...
Dogan, Gulden; Yılmaz, Aslı; Bagci, Eyup; Kaya, Zeki (2017-12-01)
The phylogenetic relationships of Drosanthe section of Hypericum genus (Hypericaceae) were analyzed by using non-coding chloroplast DNA region (trnL 3'-trnF) for 58 individuals. The section is represented by 23 taxa and nine of which are endemic to Turkey. The chloroplast phylogeny suggested that the members of this section belonged to a polyphyletic group, which imply at least two independent origins. The individuals of this section clearly formed two main clades. One clade included all members of this sec...
Phylogenetic relationships between Oxytropis DC. and Astragalus L. species native to an Old World diversity center inferred from nuclear ribosomal ITS and plastid matK gene sequences
Tekpinar, Ayten Dizkirici; KARAMAN ERKUL, SEHER; AYTAÇ, ZEKİ; Kaya, Zeki (2016-01-01)
Oxytropis and Astragalus represent one of the largest angiosperm genera complexes. Although phylogenetic studies of this complex exist, the evolutionary relationships among Astragalus and Oxytropis species sharing similar habitats in the Old World have not been studied in detail. The phylogenetic relationships among 13 Oxytropis and 56 Astragalus species native to Turkey were inferred from nucleotide sequence variations in the nuclear ribosomal internal transcribed spacer (ITS) and chloroplast maturase-like...
KENCE, M; Kence, Aykut (1992-10-01)
The pattern of inheritance of genes conferring resistance to malathion and genetic consequences of linkage between an autosomal male-determining factor and resistance genes on the second chromosome were investigated in a strain of house fly, Musca domestica L., selected for malathion resistance. The second and fifth chromosomes contribute significantly to malathion resistance. The presence of a male-determining factor linked with the resistance genes on the second chromosome resulted in a strong sexual dimo...
Citation Formats
A. Tekpinar, S. KARAMAN ERKUL, Z. AYTAÇ, and Z. Kaya, “Phylogenetic relationships among native Oxytropis species in Turkey using the trnL intron, trnL-F IGS, and trnV intron cpDNA regions,” TURKISH JOURNAL OF BOTANY, pp. 472–479, 2016, Accessed: 00, 2020. [Online]. Available: