A novel conducting copolymer: Investigation of its matrix properties for cholesterol biosensor applications

Date

2013-06-01

Author

SÖYLEMEZ, SANİYE
Kanik, Fulya Ekiz
Nurioglu, Ayda Goycek
Akpinar, Hava
Toppare, Levent Kamil

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2-Heptyl-4,7-di(thiophen-2-yl)-1H-benzo[d]imidazole) (BImTh) was synthesized. Electrochemical copolymerization of this monomer with 2-(((9H-fluoren-9-yl)methoxy)carbonylamino)acetic acid (Fmoc-Gly-OH) was achieved on a graphite electrode and used as a matrix for amperometric cholesterol biosensing studies. In order to prepare a new cholesterol biosensor, cholesterol oxidase (ChOx) was covalently immobilized onto the copolymer coated graphite electrode. Cholesterol was used as the substrate and the decrease in oxygen level as a result of enzymatic reaction was monitored at -0.7V vs Ag reference electrode in a phosphate buffer (50 mM, pH 7.0). Kinetic parameters, storage stabilities and surface characteristics were investigated. K-M(app), I-max, LOD and sensitivity were calculated as 6.25 mu M, 9.69 mu A, 0.17 mu M and 2.47 mA/mM cm(2), respectively. This biosensor was applied to the determination of total cholesterol in serum samples. The estimation of cholesterol is an important for the diagnosis and prevention of several heart diseases and arteriosclerosis. Hence, it is important to develop new cholesterol biosensors.

Subject Keywords

Instrumentation, Electrical and Electronic Engineering, Materials Chemistry, Electronic, Optical and Magnetic Materials, Surfaces, Coatings and Films, Condensed Matter Physics, Metals and Alloys

URI

https://hdl.handle.net/11511/42403

Journal

SENSORS AND ACTUATORS B-CHEMICAL

DOI

https://doi.org/10.1016/j.snb.2013.03.009

Collections

Department of Chemistry, Article

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Citation Formats

S. SÖYLEMEZ, F. E. Kanik, A. G. Nurioglu, H. Akpinar, and L. K. Toppare, “A novel conducting copolymer: Investigation of its matrix properties for cholesterol biosensor applications,” SENSORS AND ACTUATORS B-CHEMICAL, pp. 322–329, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42403.