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A new amperometric cholesterol biosensor based on poly(3,4-ethylenedioxypyrrole)
Date
2009-03-02
Author
Turkarslan, Ozlem
Kayahan, Senem
Toppare, Levent Kamil
Metadata
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Cholesterol oxidase (ChOx) was physically entrapped in poly(3,4-ethylenedioxypyrrole) (PEDOP) to construct an amperometric cholesterol biosensor. The responses of the enzyme electrodes were measured via monitoring oxidation current of H2O2 at +0.7V in the absence of a mediator. Kinetic parameters, operational and storage stabilities, pH and temperature dependencies were determined. K-m, I-max and sensitivity (I-max/K-m) were calculated as 3.4 mM, 34 mu A cm(-2) and 10 mu A mM(-1) cm(-2), respectively. The minimum detectable substrate concentration was 0.4 mM and for a period of 20 days the biosensor showed the maximum relative activity.
Subject Keywords
Instrumentation
,
Electrical and Electronic Engineering
,
Materials Chemistry
,
Electronic, Optical and Magnetic Materials
,
Surfaces, Coatings and Films
,
Condensed Matter Physics
,
Metals and Alloys
URI
https://hdl.handle.net/11511/40369
Journal
SENSORS AND ACTUATORS B-CHEMICAL
DOI
https://doi.org/10.1016/j.snb.2008.10.016
Collections
Department of Chemistry, Article