A new amperometric cholesterol biosensor based on poly(3,4-ethylenedioxypyrrole)

Date

2009-03-02

Author

Turkarslan, Ozlem
Kayahan, Senem
Toppare, Levent Kamil

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

111
views

0
downloads

Cholesterol oxidase (ChOx) was physically entrapped in poly(3,4-ethylenedioxypyrrole) (PEDOP) to construct an amperometric cholesterol biosensor. The responses of the enzyme electrodes were measured via monitoring oxidation current of H2O2 at +0.7V in the absence of a mediator. Kinetic parameters, operational and storage stabilities, pH and temperature dependencies were determined. K-m, I-max and sensitivity (I-max/K-m) were calculated as 3.4 mM, 34 mu A cm(-2) and 10 mu A mM(-1) cm(-2), respectively. The minimum detectable substrate concentration was 0.4 mM and for a period of 20 days the biosensor showed the maximum relative activity.

Subject Keywords

Instrumentation, Electrical and Electronic Engineering, Materials Chemistry, Electronic, Optical and Magnetic Materials, Surfaces, Coatings and Films, Condensed Matter Physics, Metals and Alloys

URI

https://hdl.handle.net/11511/40369

Journal

SENSORS AND ACTUATORS B-CHEMICAL

DOI

https://doi.org/10.1016/j.snb.2008.10.016

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

A novel conducting copolymer: Investigation of its matrix properties for cholesterol biosensor applications SÖYLEMEZ, SANİYE; Kanik, Fulya Ekiz; Nurioglu, Ayda Goycek; Akpinar, Hava; Toppare, Levent Kamil (Elsevier BV, 2013-06-01) 2-Heptyl-4,7-di(thiophen-2-yl)-1H-benzo[d]imidazole) (BImTh) was synthesized. Electrochemical copolymerization of this monomer with 2-(((9H-fluoren-9-yl)methoxy)carbonylamino)acetic acid (Fmoc-Gly-OH) was achieved on a graphite electrode and used as a matrix for amperometric cholesterol biosensing studies. In order to prepare a new cholesterol biosensor, cholesterol oxidase (ChOx) was covalently immobilized onto the copolymer coated graphite electrode. Cholesterol was used as the substrate and the decrease ...
Understanding the role ofd-Alluloseand soy protein addition in pectin gels Ates, Elif Gokcen; ÖZVURAL, EMİN BURÇİN; Öztop, Halil Mecit (Wiley, 2020-09-01) D-Allulose (a monosaccharide and C3 epimer of fructose), one of the common rare sugars is getting attention due to its low caloric values. In this study, DAllulose was used as a replacement of sucrose at different ratios (D-Allulose/Sucrose: 35/0, 20/15, 10/25, 0/35) to formulate pectin-based soft confectionery gels. Soy protein isolate was also added to increase the protein content. Physical properties, such as hardness, moisture content, pH, and color, were measured for the gels. Higher hardness values we...
An engineered pancreatic cancer model with intra-tumoral heterogeneity of driver mutations Moon, Hye-ran; Ozcelikkale, Altug; Yang, Yi; Elzey, Bennett D.; Konieczny, Stephen F.; Han, Bumsoo (Royal Society of Chemistry (RSC), 2020-10-01) Pancreatic ductal adenocarcinoma (PDAC) is a complex disease with significant intra-tumoral heterogeneity (ITH). Currently, no reliable PDAC tumor model is available that can present ITH profiles in a controlled manner. We develop an in vitro microfluidic tumor model mimicking the heterogeneous accumulation of key driver mutations of human PDAC using cancer cells derived from genetically engineered mouse models. These murine pancreatic cancer cell lines have KPC (Kras and Trp53 mutations) and KIC genotypes ...
A soluble conducting polymer of 2,5-di(thiophen-2-yl)-1-p-tolyl-1H-pyrrole and its electrochromic device YİGİTSOY, Basak; VARİS, Serhat; Tanyeli, Cihangir; Akhmedov, Idris M.; Toppare, Levent Kamil (Elsevier BV, 2007-02-26) A monomer 2,5-di(thiophen-2-yl)-1-p-tolyl-1H-pyrrole was synthesized via reaction of 1,4-di(2-thienyl)-1,4-butanedione and p-toluidine in the presence of catalytical amount of p-toluenesulfonic acid. Chemical polymerization of the monomer yielded a soluble polymer. The average molecular weight was determined by gel permeation chromatography as number average molecular weight (Mn) 2.5 x 10(3) g/mol. The monomer was also electrochemically polymerized in the presence of LiClO4, NaClO4 (1:1) as the supporting e...
PREPARATION AND CHARACTERIZATION OF POLYMER COATED SMALL UNILAMELLAR VESICLES OZDEN, MY; Hasırcı, Vasıf Nejat (1991-09-02) Glucose oxidase was entrapped in small unilamellar vesicles composed of phosphatidylcholine, dicetyl phosphate and cholesterol. Prediction of the enzyme content of liposomes by calculations based on input concentrations of lipid and protein, dimensions of the lipids and the liposomes yielded one protein per vesicle. The entrapment efficiency was experimentally determined to be about 13%. On the other hand the entrapment efficiency for the small chromate ions was found to be significantly lower (0.1%). T...

Citation Formats

O. Turkarslan, S. Kayahan, and L. K. Toppare, “A new amperometric cholesterol biosensor based on poly(3,4-ethylenedioxypyrrole),” SENSORS AND ACTUATORS B-CHEMICAL, pp. 484–488, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40369.