A new amperometric cholesterol biosensor based on poly(3,4-ethylenedioxypyrrole)

2009-03-02
Turkarslan, Ozlem
Kayahan, Senem
Toppare, Levent Kamil
Cholesterol oxidase (ChOx) was physically entrapped in poly(3,4-ethylenedioxypyrrole) (PEDOP) to construct an amperometric cholesterol biosensor. The responses of the enzyme electrodes were measured via monitoring oxidation current of H2O2 at +0.7V in the absence of a mediator. Kinetic parameters, operational and storage stabilities, pH and temperature dependencies were determined. K-m, I-max and sensitivity (I-max/K-m) were calculated as 3.4 mM, 34 mu A cm(-2) and 10 mu A mM(-1) cm(-2), respectively. The minimum detectable substrate concentration was 0.4 mM and for a period of 20 days the biosensor showed the maximum relative activity.
SENSORS AND ACTUATORS B-CHEMICAL

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Citation Formats
O. Turkarslan, S. Kayahan, and L. K. Toppare, “A new amperometric cholesterol biosensor based on poly(3,4-ethylenedioxypyrrole),” SENSORS AND ACTUATORS B-CHEMICAL, pp. 484–488, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40369.