A new amperometric cholesterol biosensor based on poly(3,4-ethylenedioxypyrrole)

Date

2009-03-02

Author

Turkarslan, Ozlem
Kayahan, Senem
Toppare, Levent Kamil

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Cholesterol oxidase (ChOx) was physically entrapped in poly(3,4-ethylenedioxypyrrole) (PEDOP) to construct an amperometric cholesterol biosensor. The responses of the enzyme electrodes were measured via monitoring oxidation current of H2O2 at +0.7V in the absence of a mediator. Kinetic parameters, operational and storage stabilities, pH and temperature dependencies were determined. K-m, I-max and sensitivity (I-max/K-m) were calculated as 3.4 mM, 34 mu A cm(-2) and 10 mu A mM(-1) cm(-2), respectively. The minimum detectable substrate concentration was 0.4 mM and for a period of 20 days the biosensor showed the maximum relative activity. (C) 2008 Elsevier B.V. All rights reserved.

Subject Keywords

Instrumentation, Electrical and Electronic Engineering, Materials Chemistry, Electronic, Optical and Magnetic Materials, Surfaces, Coatings and Films, Condensed Matter Physics, Metals and Alloys

URI

https://hdl.handle.net/11511/40369

Collections

Department of Chemistry, Article