In Vitro Selection of DNA Aptamers to Glioblastoma Multiforme

Date

2011-03-01

Author

Bayrac, Abdullah Tahir
Sefah, Kwame
Parekh, Parag
Bayrac, Ceren
Gulbakan, Basri
Öktem, Hüseyin Avni
Tan, Weihong

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Aptamer probes for specific recognition of glioblastoma multiforme were generated using a repetitive and broad cell-SELEX-based procedure without negative selection. The 454 sequencing technology was used to monitor SELEX, and bioinformatics tools were used to identify aptamers from high throughput data. A group of aptamers were generated that can bind to target cells specifically with dissociation constants (K-d) in the nanomolar range. Selected aptamers showed high affinity to different types of glioblastoma cell lines while showing little or no affinity to other cancer cell lines. The aptamers generated in this study have potential use in different applications, such as probes for diagnosis and devices for targeted drug delivery, as well as tools for molecular marker discovery for glioblastomas.

Subject Keywords

DNA aptamer, Cell-SELEX, Glioblastoma cancer

URI

https://hdl.handle.net/11511/42819

Journal

ACS CHEMICAL NEUROSCIENCE

DOI

https://doi.org/10.1021/cn100114k

Collections

Department of Biology, Article

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Citation Formats

A. T. Bayrac et al., “In Vitro Selection of DNA Aptamers to Glioblastoma Multiforme,” ACS CHEMICAL NEUROSCIENCE, pp. 175–181, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42819.