Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Synthesis and characterization of nanosized calcium phosphates by flame spray pyrolysis, and their effect on osteogenic differentiation of stem cells
Download
index.pdf
Date
2015-02-14
Author
Ataol, Sibel
Tezcaner, Ayşen
Duygulu, Ozgur
Keskin, Dilek
Machin, Nesrin E.
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
225
views
0
downloads
Cite This
The present study evaluates the synthesis of biocompatible osteoconductive and osteoinductive nano calcium phosphate (CaP) particles by industrially applied, aerosol-derived flame spray pyrolysis method for biomedical field. Calcium phosphate nanoparticles were produced in a range of calcium-to-phosphorus ratio, (1.20-2.19) in order to analyze the morphology and crystallinity changes, and to test the bioactivity of particles. The characterization results confirmed that nanometer-sized, spherical calcium phosphate particles were produced. The average primary particle size was determined as 23 nm by counting more than 500 particles in TEM pictures. XRD patterns, HRTEM, SAED, and SEM analyses revealed the amorphous nature of the asprepared nano calcium phosphate particles at low Ca/P ratios. Increases in the specific surface area and crystallinity were observed with the increasing Ca/P ratio. TGA-DTA analysis showed that the thermally stable crystal phases formed after 700 degrees C. Cell culture studies were conducted with urine-derived stem cells that possess the characteristics of mesenchymal stem cells. Synthesized amorphous nanoparticles did not have cytotoxic effect at 5-50 mu g/ml concentration range. Cells treated with the as-prepared nanoparticles had higher alkaline phosphatase (ALP) enzyme activity than control cells, indicating osteogenic differentiation of cells. A slight decrease in ALP activity of cells treated with two highest Ca:P ratios at 50 mu g/ml concentration was observed at day 7. The findings suggest that calcium phosphate nanoparticles produced in this work have a potential to be used as biomaterials in biomedical applications.
Subject Keywords
Flame spray pyrolysis
,
Nanoparticles
,
Calcium phosphates
,
USC
,
Osteogenic differentiation
,
Nanobiotechnology
,
Nanomedicine
URI
https://hdl.handle.net/11511/43683
Journal
JOURNAL OF NANOPARTICLE RESEARCH
DOI
https://doi.org/10.1007/s11051-015-2901-0
Collections
Department of Engineering Sciences, Article
Suggestions
OpenMETU
Core
Synthesis of iron borophosphates and phosphates with zeo-type structures
Tuncel, Selcan; Kızılyallı, Meral; Department of Chemistry (2004)
New iron phosphate and borophosphate compounds were synthesized and characterized by single crystal/powder X-ray diffraction, infrared spectroscopy, raman spectroscopy, thermogravimetric analysis, electron microscopy and elemental analysis. Using several compositions, Fey B(PO4)x type of compounds were attempted to be prepared by solid state reactions. The solid state reactions of boron compounds with a phosphating agent has been completed at 950oC. A new product Fe2BP3O12 is synthesized and indexed in this...
Fabrication and characterization of porous hydroxyapatite and biphasic calcium phosphate ceramic as bone substitutes
Koc, N; Timucin, M; Korkusuz, Feza (2004-01-01)
In the present study, the calcium phosphate powders (HAP, HAP-TCP) were produced by a precipitation technique involving the use of aqueous solutions of calcium nitrate and diammonium hydrogen phosphate. Preforms of calcium phosphate ceramics were produced by using a modified slip casting technique. Porosity in the cast and in the sintered ceramic was obtained through custom made PMMA beads similar to200-300 mum size. The green ceramic samples were prepared by suspending the apatite powder and PMMA beads in ...
Preparation and thermal characterization of poly(2-vinylpyridine) copolymers coordinated to Cr nanoparticles
Öztürk, Yurdagül; Kayran, Ceyhan; Hacaloğlu, Jale (2015-06-01)
In this study, polystyrene-block-poly(2-vinylpyridine), PS-b-P2VP, polyisoprene-block-poly(2-vinylpyridne), PI-b-P2VP and poly(methyl metacrylate)-block-poly(2-vinylpyridine), PMMA-b-P2VP, coordinated to Cr metal were synthesized and characterized by Fourier transform infrared, transmission electron microscopy and direct pyrolysis mass spectrometry techniques. Both thermal degradation mechanism and thermal stability of P2VP blocks were affected by the coordination of Cr nanoparticles to nitrogen of pyridine...
Characterization of doped polypyrrole-poly(methylthienyl methacrylate) films via pyrolysis mass spectrometry
Athar, Imran; Hacaloğlu, Jale; Toppare, Levent Kamil (2004-07-01)
This work presents the characterization of p-toluene sulfonate doped polypyrrole-poly(methylthienyl methacrylate) films synthesized by electrochemical polymerization of pyrrole on a poly(methylthienyl methacrylate) coated anode. Pyrolysis mass spectrometry was used to characterize the polymer. The study was focused on the influence of each of the components on the thermal degradation behaviour of the other. The results indicated that decomposition of the matrix polymer, ie poly(methylthienyl methacrylate), ...
Synthesis of calcium carbonate particles for biomedical applications
Oral, Çağatay Mert; Ercan, Batur; Department of Metallurgical and Materials Engineering (2020)
Calcium carbonate (CaCO3) particles have been widely used in biomedical applications owing to their biocompatibility and biodegradability. In order to effectively utilize CaCO3 particles in biomedical applications, their physical and chemical properties should be systematically controlled. However, this is a challenging task due to the presence of three different anhydrous CaCO3 polymorphs having complex crystallization behavior. In this thesis, CaCO3 particles were synthesized at distinct environments to c...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
S. Ataol, A. Tezcaner, O. Duygulu, D. Keskin, and N. E. Machin, “Synthesis and characterization of nanosized calcium phosphates by flame spray pyrolysis, and their effect on osteogenic differentiation of stem cells,”
JOURNAL OF NANOPARTICLE RESEARCH
, pp. 0–0, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/43683.