Increased production of Coleoptera-specific delta-endotoxin by homologous expression of cry3Aa11 in Bacillus thuringiensis Mm2

Kurt, Aslihan
Özcengiz, Gülay
The cry3Aa11 gene of Bacillus thuringiensis strain Mm2 (B. thuringiensis Mm2) was PCR-amplified and cloned into E. coli/B. thuringiensis shuttle vector pHT315. The recombinant plasmid carrying the cry3Aa11 gene was introduced into the parental B. thuringiensis Mm2. The toxin production capacities of parental and recombinant strains grown in DSM, containing either 50 mM or 200 mM inorganic phosphate (Pi), were compared by SDS-PAGE and western blot analyses. In comparison to the parental strain, recombinant B. thuringiensis Mm2 produced 4-fold more Cry3Aa11 toxin after 12 h of growth in the presence of 50 mM Pi. However, the 200 mM Pi concentration did not cause a drastic increase in toxin production by the recombinant strain. This study shows that higher toxin production could be obtained from the cultures during the early hours of growth by introducing the multicopy cry3Aa11 gene into the source organism.


Molecular cloning, characterization, and homologous expression of an endochitinase gene from Bacillus thuringiensis serovar morrisoni
Okay, Sezer; Özcengiz, Gülay (2011-01-01)
The endochitinase gene (chi3023) of Bacillus thuringiensis (Bt) serovar morrisoni strain 3023 was amplified via polymerase chain reaction (PCR) and cloned in Escherichia coli. The ORF of chi3023 (GenBank Accession Number: DQ993175) consists of 2031 nucleotides encoding a 676-residue protein with a calculated molecular mass of 74.5 kDa and a pI value of 6.0. The amino acid sequence of Chi3023 was compared with previously sequenced Bt chitinases and the phylogenetic relationships among them were determined. T...
Increasing clavulanic acid production both in wild type and industrial streptomyces clavuligerus strains by amplification of positive regulator claR gene
Mutlu, Alper; Özcengiz, Gülay; Department of Biology (2012)
Streptomyces clavuligerus is a Gram-positive, filamentous bacterium which produces several important secondary metabolites, including isopenicillin N, cephamycin C and the β-lactamase inhibitor clavulanic acid. Among these compounds, clavulanic acid is being used in combination with commonly used β-lactam antibiotics in order to fight against bacterial infections that are resistant to such antibiotics. Among these combinations, Augmentin, composed of amoxicillin and clavulanic acid, is the most widely presc...
Expression and activity analyses of industrially important extracellular enzymes produced by a bacilysin knock-out mutant of Bacillus Subtilis
Aytekin, Samet; Özcengiz, Gülay; Okay, Sezer; Department of Molecular Biology and Genetics (2018)
Bacilysin, the smallest peptide antibiotic known to date, is produced non-ribosomally by Bacillus subtilis by the collective actions of seven proteins transcribed from bacABCDEF operon and bacG gene. Bacilysin is a two-amino acid peptide composed of L-alanine and a modified amino acid, L-anticapsin. Bacilysin biosynthesis was shown to be strongly regulated by quorum sensing through the actions of global regulator proteins including Spo0K, Spo0H, Spo0A, ComQ/ComX, ComP/ComA as well as several Phr proteins, O...
New Steroidal 4-Aminoquinolines Antagonize Botulinum Neurotoxin Serotype A in Mouse Embryonic Stem Cell Derived Motor Neurons in Postintoxication Model.
Konstantinović, J; Kiriş, Erkan; Kota, KP; Kugelman-Tonos, J; Videnović, M; Cazares, LH; Terzić, Jovanović; Verbić, TŽ; Andjelković, B; Duplantier, AJ; Bavari, S; Šolaja, BA (American Chemical Society (ACS), 2018-02-22)
The synthesis and inhibitory potencies against botulinum neurotoxin serotype A light chain (BoNT/A LC) using in vitro HPLC based enzymatic assay for various steroidal, benzothiophene, thiophene, and adamantane 4-aminoquinoline derivatives are described. In addition, the compounds were evaluated for the activity against BoNT/A holotoxin in mouse embryonic stem cell derived motor neurons. Steroidal derivative 16 showed remarkable protection (up to 89% of uncleaved SNAP-25) even when administered 30 min postin...
Stability of a recombinant plasmid carrying alpha-amylase gene in Bacillus subtilis
Gültekin, Güzin Candan; Bayındırlı, Alev (1998-03-01)
Plasmid pSB6 is a streptococcal recombinant plasmid carrying the alpha-amylase gene of Bacillus amyloliquefaciens and the chloramphenicol resistance gene. The segregational and structural instabilities of this plasmid were examined under non-selective conditions in Bacillus subtilis. These instabilities were modelled according to a kinetic expression derived from the difference in the growth between plasmid-bearing and plasmid-free cells. This plasmid showed slight segregational instability and much higher ...
Citation Formats
A. Kurt and G. Özcengiz, “Increased production of Coleoptera-specific delta-endotoxin by homologous expression of cry3Aa11 in Bacillus thuringiensis Mm2,” TURKISH JOURNAL OF BIOLOGY, pp. 585–592, 2011, Accessed: 00, 2020. [Online]. Available: