Isolation, identification and characterization of non-saccharomyces and saccharomyces yeasts in traditional wines made from five different national grapes in Turkey

Download

index.pdf

Date

2019

Author

Aktuna, İpe

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

333
views

213
downloads

Kalecik Karası, Öküzgözü, Dimrit and Boğazkere are local red grapes in Ankara, Elazığ, Cappadocia and Elazığ, respectively. Moreover, Emir is a local white grape grown in Cappadocia. Yeast populations are different in various types of grapes due to the unique properties of these grapes. In this study, 104 non-Saccharomyces and 293 Saccharomyces yeasts were isolated at different maceration and fermentation times from the grape’s musts and wines made of these grapes. All 104 non-Saccharomyces isolates were sequenced. 29 (among 77 sequenced isolates) out of 293 total Saccharomyces yeasts were selected as those with high technological properties. Among all 181 (104 non-Saccharomyces and 77 Saccharomyces) yeast isolates, 16 different species of yeasts were identified by DNA sequencing of internal transcribed spacer (ITS) region and/or D1/D2 domain of large subunit ribosomal DNA as: Hanseniaspora guilliermondii, Hanseniaspora opuntiae, Hanseniaspora uvarum, Rhodotorula mucilaginosa, Wickerhamomyces anomalus, Metschnikowia aff. fructicola, Metschnikowia fructicola, Lachancea thermotolerans, Solicoccozyma aeria, Metschnikowia aff. pulcherrima, Metschnikowia pulcherrima, Metschnikowia Kalecik Karası, Öküzgözü, Dimrit and Boğazkere are local red grapes in Ankara, Elazığ, Cappadocia and Elazığ, respectively. Moreover, Emir is a local white grape grown in Cappadocia. Yeast populations are different in various types of grapes due to the unique properties of these grapes. In this study, 104 non-Saccharomyces and 293 Saccharomyces yeasts were isolated at different maceration and fermentation times from the grape’s musts and wines made of these grapes. All 104 non-Saccharomyces isolates were sequenced. 29 (among 77 sequenced isolates) out of 293 total Saccharomyces yeasts were selected as those with high technological properties. Among all 181 (104 non-Saccharomyces and 77 Saccharomyces) yeast isolates, 16 different species of yeasts were identified by DNA sequencing of internal transcribed spacer (ITS) region and/or D1/D2 domain of large subunit ribosomal DNA as: Hanseniaspora guilliermondii, Hanseniaspora opuntiae, Hanseniaspora uvarum, Rhodotorula mucilaginosa, Wickerhamomyces anomalus, Metschnikowia aff. fructicola, Metschnikowia fructicola, Lachancea thermotolerans, Solicoccozyma aeria, Metschnikowia aff. pulcherrima, Metschnikowia pulcherrima, Metschnikowia sinensis, Metschnikowia chrysoperlae, Starmerella bacillaris, Saccharomyces cerevisiae and Saccharomyces cf. cerevisiae/paradoxus. Selected non-Saccharomyces isolates were tested for some phenotypic properties such as alcohol tolerance, SO2 tolerance and H2S production. In addition, selected Lachancea thermotolerans and Saccharomyces cerevisiae were used as starter cultures for sequential inoculation to produce Emir wine. Moreover, Hanseniaspora uvarum and Saccharomyces cerevisiae were added as a single starter culture separately for wine making from Kalecik Karası grapes. Volatile compounds of these wines were analyzed by gas chromatography-mass spectrometry (GS-MS). According to flavour profiles and amounts of volatile compounds, Emir wines with sequential inoculation of our Lachancea thermotolerans and Saccharomyces cerevisiae strains were found successful.

Subject Keywords

Saccharomyces, Saccharomyces Biotechnology., Non-Saccharomyces, Saccharomyces, wines starter cultures, identification, aroma compounds

URI

http://etd.lib.metu.edu.tr/upload/12623619/index.pdf
https://hdl.handle.net/11511/43853

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Stratigraphy of the volcanic products around Nemrut caldera: Implications for reconstruction of the caldera formation Karaoglu, O; Ozdemir, Y; Tolluoglu, AU; Karabiyikoglu, M; Kose, O; Froger, JL (2005-05-01) The volcanological development of the Nemrut stratovolcano, located near the southwestern tip of Lake Van in eastern Turkey, is subdivided into three stages: pre-caldera, post-caldera and late stages. Two ignimbrite flows have been recognized in the pre-caldera stage. The earlier of the two occurred after basaltic lavas formed along extensional fissures. The latter, which forms the main subject of this paper, was a major flow associated with the development of the Nemrut caldera. The pyroclastic deposits of...
Characterization and Comparison of Turkish Table Olive Varieties With NMR Relaxometry and Magnetic Resonance Imaging Kilercioğlu, Mete; Özel, Barış; Öztop, Halil Mecit (2016-01-01) Olive is one of the fruits that is mostly consumed in the Mediterranean region. Depending on the variety, oil quality of the olive changes significantly. In this study, Nuclear Magnetic Resonance (NMR), Relaxometry and Magnetic Resonance Imaging (MRI) experiments were used to characterize different Turkish table olive varieties, (Ayvalik, Mega, Sele, Light) in terms of tissue structure, fat and water contents. Moisture and fat content were measured using an infrared moisture analyzer and soxhlet extraction,...
Determination of phenolic compounds content and antioxidant activity in skin, pulp, seed, cane and leaf of five native grape cultivars in West Azerbaijan province, Iran Farhadi, Khalil; Esmaeilzadeh, Forough; Hatami, Mehdi; Forough, Mehrdad; Molaie, Rahim (2016-05-01) In the present work, the phenolic compounds content and antioxidant activity in the skin, pulp, seed, cane and leaf of one international (Muscat) and five native (Hosseini, Ghara Shira, Agh Shani, Ghara Shani and Ghara Ghandome) grape cultivated in West Azerbaijan, Iran were investigated. Ghara Shani grape skin was found to contain the highest content of total phenolic and anthocyanin and cane of Ghara Shani contains the highest amount of flavonoid. A remarkable DPPH radical scavenging activity up to 95% an...
Impact of olive grove management on spider (araneae) community in Northwestern Turkey Elverici, Mert; Bilgin, Cemal Can; Department of Biology (2018) Olive (Olea europea) plantations cover vast areas on the Aegean coast of Turkey, including the southern foothills of Kaz Mountains. In such environments, olive trees dominate the terrestrial vegetation in such a way that they might form a novel ‘ecosystem’. The ‘olive grove agro-ecosystem’ is shaped by agricultural management while impacts on biodiversity are largely unknown. As representatives of local biodiversity, spider communities were sampled in soil and vegetation microhabitats, alongside with variou...
Comparative sequence analysis of the internal transcribed spacer 2 region of Turkish red pine (pinus brutia ten.) and natural aleppo pine (pinus halepensis mill.) populations from Turkey Tozkar, C. Özge; Kaya, Zeki; Department of Biology (2007) Turkish red pine (Pinus brutia) is wide-spread and an important forest tree species in Turkey, occurring mainly in southern, western and north-western Turkey and as small isolated populations in the Black Sea region. Aleppo pine (Pinus halepensis) has naturally found only in Adana and Muğla provinces as small population in mixture with Turkish red pine. Although Turkish red pine and Aleppo pine are morphologically different, Turkish red pine has been regarded as subspecies of Aleppo pine by some taxonomists...

Citation Formats

İ. Aktuna, “Isolation, identification and characterization of non-saccharomyces and saccharomyces yeasts in traditional wines made from five different national grapes in Turkey,” Thesis (M.S.) -- Graduate School of Natural and Applied Sciences. Biotechnology., Middle East Technical University, 2019.