Investigating SNX3 binding motif on EGFR

Download
2019
Ercan, Melda
Sorting Nexin 3 (SNX3) is a protein that is involved in retromer complex and has a role in recognition and trafficking of retromer cargo proteins. SNX3-retromer provides the recycling of their cargo proteins back to the plasma membrane or to Trans Golgi Network. Recent studies reveal the specificity of SNX3 in cargo recognition by acting on a recycling motif on receptor cytoplasmic tails. Considering the importance of SNX3 in receptor recycling, we hypothesized that EGFR can be one of the cargo proteins that is recognized and recycled by the SNX3-retromer. We used site-directed mutagenesis, Co-IP techniques to investigate a potential link between SNX3 and EGFR. Preliminary findings from this work will guide future experiments to further investigate protein-protein interaction.