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A parylene-based dual channel micro-electrophoresis system for rapid mutation detection via heteroduplex analysis
Date
2008-09-01
Author
SUKAS, Sertan
Erson Bensan, Ayşe Elif
Sert, Cüneyt
Külah, Haluk
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A new dual channel micro-electrophoresis system for rapid mutation detection based on heteroduplex analysis was designed and implemented. Mutation detection was successfully achieved in a total separation length of 250 pm in less than 3 min for a 590 by DNA sample harboring a 3 bp mutation causing an amino acid change. Parylene-C was used as the structural material for fabricating the micro-channels as it provides conformal deposition, transparency, biocompatibility, and low background fluorescence without any surface treatment. A new dual channel architecture was derived from the traditional cross-channel layout by forming two identical channels with independent sample loading and waste reservoirs. The control of injected sample volume was accomplished by a new u-turn injection technique with pull-back method. The use of heteroduplex analysis as a mutation detection method on a cross-linked polyacrylamide medium provided accurate mutation detection in an extremely short length and time. The presence of two channels on the microchip offers the opportunity of comparing the sample to be tested with a desired control sample rapidly, which is very critical for the accuracy and reliability of the mutation analyses, especially for clinical and research purposes.
Subject Keywords
Clinical Biochemistry
,
Biochemistry
URI
https://hdl.handle.net/11511/45873
Journal
ELECTROPHORESIS
DOI
https://doi.org/10.1002/elps.200800164
Collections
Department of Biology, Article
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S. SUKAS, A. E. Erson Bensan, C. Sert, and H. Külah, “A parylene-based dual channel micro-electrophoresis system for rapid mutation detection via heteroduplex analysis,”
ELECTROPHORESIS
, pp. 3752–3758, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/45873.