Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
PURIFICATION AND CHARACTERIZATION OF 2 FORMS OF SOLUBLE NADH CYTOCHROME-B5 REDUCTASES FROM HUMAN ERYTHROCYTES
Date
1992-01-01
Author
ARINC, E
Güray, Nülüfer Tülün
SAPLAKOGLU, U
Adalı, Orhan
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
274
views
0
downloads
Cite This
1. Two forms of soluble NADH cytochrome b5 reductase were purified from human erythrocytes. Two distinct fractions both having the NADH cytochrome b5 reductase activity eluted from the second DEAE-cellulose column were further purified by ultrafiltration and 5'-ADP-agarose affinity chromatography.
Subject Keywords
Biochemistry
,
Physiology
,
Molecular Biology
,
General Medicine
URI
https://hdl.handle.net/11511/45905
Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
DOI
https://doi.org/10.1016/0305-0491(92)90185-t
Collections
Department of Biology, Article
Suggestions
OpenMETU
Core
Synthesis, Characterization and Evaluation of Cytotoxic Activities of Novel Aziridinyl Phosphonic Acid Derivatives
Khan, Rehan; Ulusan, Sinem; Banerjee, Sreeparna; Doğan, Özdemir (Wiley, 2019-11-01)
New aziridine 2-phosphonic acids were prepared by monohydrolysis of the aziridine 2-phosphonates that were obtained by the modified Gabriel-Cromwell reaction of vinyl phosphonate or alpha-tosylvinyl phosphonate with a primary amine or a chiral amine. The cellular cytotoxicity of these compounds was tested against the HCT-116 colorectal cancer cell lines and the CCD-18Co normal colon fibroblast lines using the MTT assay. Three of the synthesized phosphonic acid derivatives 2e (ethyl hydrogen {(2S)-1-[(1S)-1-...
Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure
Yildirim, Volkan; ÖZCAN, Servet; Becher, Doerte; Buettner, Knut; Hecker, Michael; Özcengiz, Gülay (Springer Science and Business Media LLC, 2011-03-09)
Background: Total soluble proteome alterations of white rot fungus Phanerochaete chrysosporium in response to different doses (25, 50 and 100 mu M) of Pb (II) were characterized by 2DE in combination with MALDI-TOF-MS.
Identification of small-molecule urea derivatives as novel NAMPT inhibitors via pharmacophore-based virtual screening
Ozgencil, Fikriye; EREN, GÖKÇEN; ÖZKAN, YEŞİM; GÜNTEKİN ERGÜN, SEZEN; Atalay, Rengül (Elsevier BV, 2020-01-01)
Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the condensation of nicotinamide (NAM) with 5-phosphoribosyl-1-prophosphate (PRPP) to yield nicotinamide mononucleotide (NMN), a rate limiting enzyme in a mammalian salvage pathway of nicotinamide adenine dinucleotide (NAD(+)) synthesis. Recently, intracellular NAD(+) has received substantial attention due to the recent discovery that several enzymes including poly(ADPribose) polymerases (PARPs), mono(ADP-ribose) transferases (ARTs), and sirtuins (SIR...
Characterization and modulation by drugs of sheep liver microsomal flavin monooxygenase activity
Demirdöǧen, Birsen Can; Adalı, Orhan (Wiley, 2005-07-01)
The flavin monooxygenases (FMO) catalyse the NADPH and oxygen-dependent oxidation of a wide range of nucleophilic nitrogen-, sulfur-, phosphorus-, and selenium heteroatom-containing chemicals, drugs, and agricultural agents. In the present study, sheep liver microsomal FMO activity was determined by measuring the S-oxidation rate of methimazole and the average specific activity obtained from different microsomal preparations was found to be 3.8 +/- 1.5 nmol methimazole oxidized min(-1) mg(-1) microsomal pro...
Various applications of immobilized glucose oxidase and polyphenol oxidase in a conducting polymer matrix
Cil, M.; Boyukbayram, A. E.; Kiralp, S.; Toppare, Levent Kamil; Yagci, Y. (Elsevier BV, 2007-06-01)
In this study, glucose oxidase and polyphenol oxidase were immobilized in conducting polymer matrices; polypyrrole and poly(N-(4-(3-thienyl methylene)-oxycarbonyl phenyl) maleimide-co-pyrrole) via electrochemical method. Fourier transform infrared and scanning electron microscope were employed to characterize the copolymer of (N-(4-(3-thienyl methylene)-oxycarbonyl phenyl) maleimide) with pyrrole. Kinetic parameters, maximum reaction rate and Michealis-Menten constant, were determined. Effects of temperatur...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
E. ARINC, N. T. Güray, U. SAPLAKOGLU, and O. Adalı, “PURIFICATION AND CHARACTERIZATION OF 2 FORMS OF SOLUBLE NADH CYTOCHROME-B5 REDUCTASES FROM HUMAN ERYTHROCYTES,”
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
, pp. 235–242, 1992, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/45905.