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High level multiple antibiotic resistance among fish surface associated bacterial populations in non-aquaculture freshwater environment
Date
2012-12-01
Author
ÖZAKTAŞ, Tugba
TAŞKIN, Bilgin
Gözen, Ayşe Gül
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Freshwater fish, Alburnus alburnus (bleak), were captured from Lake Mogan, situated in Ankara, during spring. The surface mucus of the fish was collected and associated bacteria were cultured and isolated. By sequencing PCR-amplified 16S RNA encoding genes, the isolates were identified as members of 12 different genera: Acinetobacter, Aeromonas, Bacillus, Brevundimonas, Gordonia, Kocuria, Microbacterium, Mycobacterium, Pseudomonas, Rhodococcus, and Staphylococcus, in addition to one strain that was unidentified. The mucus-dwelling bacterial isolates were tested for resistance against ampicillin, kanamycin, streptomycin and chloramphenicol. About 95% of the isolates were found to be resistant to ampicillin, 93% to chloramphenicol, and 88% to kanamycin and streptomycin. A Microbacterium oxydans and the unidentified environmental isolate were resistant to all four antibiotics tested at very high levels (>1600 mu g/ml ampicillin and streptomycin; >1120 mu g/ml kanamycin; >960 mu g/ml chloramphenicol). Only a Kocuria sp. was sensitive to all four antibiotics at the lowest concentrations tested (3.10 mu g/ml ampicillin and streptomycin; 2.15 mu g/ml kanamycin; 1.85 mu g/ml chloramphenicol). The rest of the isolates showed different resistance levels. Plasmid isolations were carried out to determine if the multiple antibiotic resistance could be attributed to the presence of plasmids. However, no plasmid was detected in any of the isolates. The resistance appeared to be mediated by chromosome-associated functions. This study indicated that multiple antibiotic resistance at moderate to high levels is common among the current phenotypes of the fish mucus-dwelling bacterial populations in this temperate, shallow lake which has not been subjected to any aquaculturing so far but under anthropogenic effect being in a recreational area.
Subject Keywords
Ecological Modelling
,
Waste Management and Disposal
,
Pollution
,
Water Science and Technology
URI
https://hdl.handle.net/11511/46270
Journal
WATER RESEARCH
DOI
https://doi.org/10.1016/j.watres.2012.09.010
Collections
Department of Biology, Article
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T. ÖZAKTAŞ, B. TAŞKIN, and A. G. Gözen, “High level multiple antibiotic resistance among fish surface associated bacterial populations in non-aquaculture freshwater environment,”
WATER RESEARCH
, pp. 6382–6390, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46270.