High level multiple antibiotic resistance among fish surface associated bacterial populations in non-aquaculture freshwater environment

TAŞKIN, Bilgin
Gözen, Ayşe Gül
Freshwater fish, Alburnus alburnus (bleak), were captured from Lake Mogan, situated in Ankara, during spring. The surface mucus of the fish was collected and associated bacteria were cultured and isolated. By sequencing PCR-amplified 16S RNA encoding genes, the isolates were identified as members of 12 different genera: Acinetobacter, Aeromonas, Bacillus, Brevundimonas, Gordonia, Kocuria, Microbacterium, Mycobacterium, Pseudomonas, Rhodococcus, and Staphylococcus, in addition to one strain that was unidentified. The mucus-dwelling bacterial isolates were tested for resistance against ampicillin, kanamycin, streptomycin and chloramphenicol. About 95% of the isolates were found to be resistant to ampicillin, 93% to chloramphenicol, and 88% to kanamycin and streptomycin. A Microbacterium oxydans and the unidentified environmental isolate were resistant to all four antibiotics tested at very high levels (>1600 mu g/ml ampicillin and streptomycin; >1120 mu g/ml kanamycin; >960 mu g/ml chloramphenicol). Only a Kocuria sp. was sensitive to all four antibiotics at the lowest concentrations tested (3.10 mu g/ml ampicillin and streptomycin; 2.15 mu g/ml kanamycin; 1.85 mu g/ml chloramphenicol). The rest of the isolates showed different resistance levels. Plasmid isolations were carried out to determine if the multiple antibiotic resistance could be attributed to the presence of plasmids. However, no plasmid was detected in any of the isolates. The resistance appeared to be mediated by chromosome-associated functions. This study indicated that multiple antibiotic resistance at moderate to high levels is common among the current phenotypes of the fish mucus-dwelling bacterial populations in this temperate, shallow lake which has not been subjected to any aquaculturing so far but under anthropogenic effect being in a recreational area.


Multiple antibiotic resistance of surface mucus dwelling bacterial populations in freshwater fish
Özaktaş, Tuğba; Gözen, Ayşe Gül; Department of Biology (2007)
Surface mucus of a freshwater fish, Alburnus alburnus (bleak), caught from Lake Mogan, situated in south of Ankara, was collected in different seasons. The total cultivable bacteria were enumerated by spread plate method on nine different media. Bacteria were isolated based on colony morphologies and pigmentation. A total of sixty bacterial isolates obtained. The mucus-dwelling bacteria were first tested for resistance against ampicillin and kanamycin; then streptomycin and chloramphenicol were added to the...
Do bigheaded carp act as a phosphorus source for phytoplankton in (sub)tropical Chinese reservoirs?
Lin, Qiuqi; Chen, Qinghang; Peng, Liang; Xiao, Lijuan; Lei, Lamei; Jeppesen, Erik (Elsevier BV, 2020-08-01)
Stocking of bigheaded carp (mainly Hypophthalmichthys nobilis and H. molitrix) is commonly used in (sub)tropical Chinese reservoirs to control phytoplankton, but with ambiguous results. Whether these carp act as a phosphorus (P) source or sink for phytoplankton is debated. We compared the trophic structures in twenty-three reservoirs with different nutrient concentrations in the flood season (after bigheaded carp introduction) with the dry season (after bigheaded carp harvesting). Fish biomass was positivel...
Combining bivalve (Corbicula fluminea) and filter-feeding fish (Aristichthys nobilis) enhances the bioremediation effect of algae: An outdoor mesocosm study
Shen, Ruijie; Gu, Xiaohong; Chen, Huihui; Mao, Zhigang; Zeng, Qingfei; Jeppesen, Erik (Elsevier BV, 2020-07-20)
In outdoor mesocosms, we experimentally studied the effect of combining two native filter feeders, bighead carp (Aristichthys nobilis) and Asian clam (Corbicula fluminea), to control nuisance cyanobacterial blooms. Four treatments - clam-only, fish-only, combined and controls - were used. In combination, bighead carp (80 g m(-3)) and Asian clam (260 g m(-2)), had a remarkable controlling effect on phytoplankton biomass and improved water quality. By the end of the experiment, the chlorophyll a (Chl a) conce...
Genetic and morphologic diversity of Pseudophoxinus (Cyprinidae): implication for conservation in Anatolia
TELLİ, MURAT; Aykut, Kence (Springer Science and Business Media LLC, 2015-01-01)
Pseudophoxinus a freshwater fish genus shows remarkable diversification with high number of endemic species in Anatolia. Most of the species of the genus are categorized as endangered for conservation in IUCN Red List due to water abstraction and habitat destruction. The aim of the study is to investigate genetic and morphological structure of Pseudophoxinus populations using allozyme, microsatellite markers and geometric morphometrics (eight landmarks). Sample collection was performed from eight localities...
Toxicity of 2,4-D acid to phytoplankton
Okay, OS; Gaines, A (Elsevier BV, 1996-03-01)
The toxic effects of 2,4-D on Phaedoactylum tricornutum (Bohlin) and Dunaliella tertiolecta (Butcher), two species of phytoplankton well suited to bioassay studies and responsive to pollutants, were studied by monitoring changes in growth in terms of cell populations, chlorophyll fluorescence and the rate of (CO2)-C-14 assimilation. Short term bioassays, batch and continuous cultures were studied. Pure 2,4-D acid appeared more toxic than the commercial amine form of the herbicide but this may have been due ...
Citation Formats
T. ÖZAKTAŞ, B. TAŞKIN, and A. G. Gözen, “High level multiple antibiotic resistance among fish surface associated bacterial populations in non-aquaculture freshwater environment,” WATER RESEARCH, pp. 6382–6390, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46270.