A facile method for the recovery of ionic liquid and lignin from biomass pretreatment

Persil Çetinkol, Özgül
In the biochemical conversion of lignocellulosic biomass to biofuels, the process of pretreatment is currently one of the most difficult and expensive operations. The use of ionic liquids (ILs) in biomass pretreatment has received considerable attention recently because of their effectiveness at decreasing biomass recalcitrance to subsequent enzymatic hydrolysis. In addition, ILs have the potential for decreasing the need for corrosive or toxic chemicals and associated waste streams that can be generated by other pretreatment methods that utilize acids and/or bases. In this article, we address two significant challenges to the realization of a practical IL pretreatment process. First, we describe a mixture containing specific proportions of a ketone and an alcohol that precipitates cellulose and lignocellulosic biomass from solutions of the IL 1-ethyl-3-methylimidazolium acetate without the formation of intermediate gel phases. Second, an IL recovery process is described that removes lignin and most residual IL solutes and that minimizes energy and solvent use. These two techniques are demonstrated by the pretreatment of 100 g of corn stover with the recovery of 89% of the initial IL and separate corn stover fractions rich in glucans, xylans, lignin, and non-polar substances. These results highlight one potential approach towards the realization of a scalable ionic liquid pretreatment process technology that enables ionic liquid recovery and reuse.


The effect of Dilution, Aeration, and Agitation on Fungal Cellulase and Xylanase Production by DDGS-based Fermentation Media in Stirred Tank Bioreactors
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-01-01)
Fungal cellulases and xylanases are commonly used for biological pretreatment of lignocellulosic biomass into simple sugars, which can then be converted into many value-added products including bioethanol. However, these enzymes are costly to produce and need a feedstock that should be more economical and high in fiber and other nutrients. Distillers dried grains with solubles (DDGS) is the byproduct of corn ethanol production and can be considered an appropriate feedstock. Among various problems in adaptat...
The impact of ionic liquid pretreatment on the chemistry and enzymatic digestibility of Pinus radiata compression wood
Compression wood represents a unique challenge for biochemical processing of softwoods to biofuels and chemicals on account of its high lignin and galactan, and low glucan contents. Here we report the impact of ionic liquid pretreatment on the chemistry and enzymatic digestibility of Pinus radiata compression wood and opposite wood. Samples were pretreated using 1-ethyl-3-methylimidazolium acetate at 120 degrees C and 155 degrees C for 3 h. Hemicelluloses were preferentially extracted during the pretreatmen...
A compact tubular photobioreactor for outdoor hydrogen production from molasses
KAYAHAN, Emine; Eroglu, Inci; Koku, Harun (2017-01-26)
Hydrogen can be produced sustainably by photofermentation of biomass. For an economically feasible operation, the process should be implemented outdoors using low-cost organic material. In the current study, molasses from a sugar factory was utilized for photofermentative hydrogen production. The experiment was run with Rhodobacter capsulatus YO3 (hup-) in fed-batch mode under outdoor conditions in Ankara between July 12, 2015 and July 24, 2015. The stacked U-tube photobioreactor (9 L) designed for outdoor ...
Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-10-01)
© 2022 Elsevier LtdLignocellulolytic enzymes such as cellulase and xylanases are needed on the industrial scales for low-cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focused on the statistical optimizat...
An unconventional approach for improving the integrity and mechanical properties of xylan type hemicellulose based films
Bahcegul, Erinc; Toraman, Hilal E.; Erdemir, Duygu; AKINALAN, Busra; Özkan, Necati; Bakir, Ufuk (2014-01-01)
The isolation of xylans from lignocellulosic biomass via alkaline extraction typically involves a neutralization step, which results in salt formation. Usually, these salts are removed from the medium to avoid their presence within the isolated xylans and films made from these polymers. The present work shows that it is not always necessary to do so, since the presence of potassium acetate (KAcO) in the films was found to be beneficial both for the film formation and mechanical properties. While desalted xy...
Citation Formats
D. DIBBLE et al., “A facile method for the recovery of ionic liquid and lignin from biomass pretreatment,” GREEN CHEMISTRY, pp. 3255–3264, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46427.