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An extracellular - Pepstatin insensitive acid protease produced by Thermoplasma volcanium
Date
2007-01-01
Author
Kocabıyık, Semra
Metadata
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In this study, some parameters for the production and caseinolytic activity of an extracellular thermostable acid protease from a thermoacidophilic archaeon Thermoplasma volcanium were determined. The highest level of growth and enzyme production were detected at pH 3.0 over an incubation period of 192 h at 60 degrees C. The pH optimum for the acid protease activity was 3.0 and the enzyme was fairly stable over a broad pH range (pH 3.0-8.0). The temperature for maximum activity of the enzyme was 55 degrees C and activity remained stable between 50 degrees C and 70 degrees C. These features could be of relevance for various biotechnological applications of this enzyme. Serine-(PMSF), cysteine-(DTT), metallo-(EDTA) and aspartate-(pepstatin) protease inhibitors did not inhibit the caseinolytic activity of the enzyme. Therefore, Tp. volcanium acid protease could be a member of the pepstatin-insensitive carboxyl proteinases.
Subject Keywords
Renewable Energy, Sustainability and the Environment
,
Environmental Engineering
,
Waste Management and Disposal
,
Bioengineering
,
General Medicine
URI
https://hdl.handle.net/11511/46489
Journal
BIORESOURCE TECHNOLOGY
DOI
https://doi.org/10.1016/j.biortech.2005.11.016
Collections
Department of Biology, Article
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S. Kocabıyık, “An extracellular - Pepstatin insensitive acid protease produced by Thermoplasma volcanium,”
BIORESOURCE TECHNOLOGY
, pp. 112–117, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46489.