Effects of plasmid curing on antibiotic susceptibility, phage type, lipopoly saccharide and outer membrane protein profiles in local Salmonella isolates

Date

2001-12-01

Author

İçgen, Bülent
Gültekin, Güzin Candan
Özcengiz, Gülay

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

198
views

0
downloads

Five different Salmonella isolates (four Salmonella enteritidis and one Salmonella havana) of human, chicken or egg origin which all expressed smooth lipopolysaccharide were subjected to plasmid curing Upon this treatment, four isolates lost at least one of their resident plasmids while all five isolates became rough in their LPS profiles, Two of the S. enteritidis isolates, CA13 and CE7 were found to convert phage types (PTs) as well. The phage type conversion in CA13 from PT6 to PT21 was found to be accompanied by the loss of a 19.0-MDa plasmid as well as the loss of sulbactam ampicillin and tetracycline resistance. The change in the PTs of the strain CE7 from PT20a to the PT20 variant was accompanied by the loss of two plasmids of 10.9 and 1.45 MDa, Outer membrane protein (OMP) type change occurred only in the isolate CA13, which lost an OMP of 27.2 kDa. The strains, CA13 and CE7 retained their high molecular weight plasmid (36 MDa), which has been very commonly found (77 of 82) among local S. enteritidis isolates. (C) 2001 Academic Press.

Subject Keywords

Food Science, Microbiology, General Medicine

URI

https://hdl.handle.net/11511/46738

Journal

FOOD MICROBIOLOGY

DOI

https://doi.org/10.1006/fmic.2001.0437

Collections

Department of Environmental Engineering, Article

Suggestions

OpenMETU
Core

Interactions of high hydrostatic pressure, pressurization temperature and pH on death and injury of pressure-resistant and pressure-sensitive strains of foodborne pathogens Alpas, Hami; BOZOGLU, F; RAY, B (Elsevier BV, 2000-09-15) The objective of this: study is to determine the interactions between high hydrostatic pressure, pressurization temperature, time and pH during pressurization on death and injury of pressure-resistant and pressure-sensitive strains of four foodborne pathogens: Staphylococcus aureus 485 and 765, Listeria monocytogenes CA and OH2, Escherichia coli O157:H7 933 and 931, Salmonella enteritidis FDA and Salmonella typhimurium E21274. Among these strains S. aureus 485, L. monocytogenes CA, E, coli O157:H7 933 and S...
Immunization of the industrial fermentation starter culture strain of Saccharomyces cerevisiae to a contaminating killer toxin-producing Candida tropicalis Izgu, F; Altinbay, D; Derinel, Y (Elsevier BV, 2004-12-01) K3 killer trait was introduced into the fermentation starter strain of Saccharomyces cerevisiae BSP 1 in order to construct immune industrial strain that produces K3 type killer toxin and was resistant to Candida tropicalis (K+) contamination. Protoplasts of respiration-deficient Rhodegrees strain of S. cerevisiae NCYC 761 (K3) and S. cerevisiae BSP 1 were fused. The resulting respiration-competent hybrid with K3 type killer activity was selected on media containing a non-fermentable carbon source and by a ...
Effect of controlled athmosphere storage,modified athmosphere packaging and gaseous ozone treatment on the survival characteristics of Salmonella enteritidis ay cherry tomatoes Daş, Elif; Gürakan, Candan; Department of Food Engineering (2004) In recent years, outbreaks of infections associated with raw and minimally processed fruits and vegetables have been reported. Possible sources for contamination are irrigation water, manure, wash water, handling by workers and contact with contaminated surfaces. Pathogens can occur on raw and minimally processed produce at populations ranging from 103 to 109 CFU/g and able to survive and sometimes grow under various storage conditions. The objective of this study was to analyse the growth/survival of Salmo...
Inactivation of Escherichia coli and Listeria innocua in kiwifruit and pineapple juices by high hydrostatic pressure Buzrul, Sencer; Alpas, Hami; Largeteau, Alain; Demazeau, Gerard (Elsevier BV, 2008-06-10) Escherichia coli and Listeria innocua in kiwifruit and pineapple juices were exposed to high hydrostatic Pressure (HHP) at 300 MPa for 5 min. Both bacteria showed equal resistance to HHR Using low (0 degrees C) or subzero (- 10 degrees C) temperatures instead of room temperature (20 degrees C) during pressurization did not change the effectiveness of HHP treatment oil both bacteria in Studied juices. Pulse pressure treatment (multiple Pulses for a total holding time of 5 min at 300 MPa) instead of continuou...
Characterization of extracellular beta-lactamases from penicillin G-resistant cells of Streptococcus thermophilus Chirica, LC; Güray, Nülüfer Tülün; Gültekin, Güzin Candan; Bozoglu, F (International Association for Food Protection, 1998-07-01) In this study, biochemical properties of two extracellular beta-lactamases produced by penicillin-resistant Streptococcus thermophilus cells were investigated. Both beta-lactamases showed specificity for penicillins but not for cephaloridins. The p-lactamases exhibited different affinities for penicillin G. The one with the higher molecular weight (F1) had a K(m) value of 3.44 mu M and a V(max), value of 8.33, mu mol/min/mg of protein, whereas the beta-lactamase with the lower molecular weight (FII) had a K...

Citation Formats

B. İçgen, G. C. Gültekin, and G. Özcengiz, “Effects of plasmid curing on antibiotic susceptibility, phage type, lipopoly saccharide and outer membrane protein profiles in local Salmonella isolates,” FOOD MICROBIOLOGY, pp. 631–635, 2001, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46738.