Immunization of the industrial fermentation starter culture strain of Saccharomyces cerevisiae to a contaminating killer toxin-producing Candida tropicalis

Date

2004-12-01

Author

Izgu, F
Altinbay, D
Derinel, Y

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K3 killer trait was introduced into the fermentation starter strain of Saccharomyces cerevisiae BSP 1 in order to construct immune industrial strain that produces K3 type killer toxin and was resistant to Candida tropicalis (K+) contamination. Protoplasts of respiration-deficient Rhodegrees strain of S. cerevisiae NCYC 761 (K3) and S. cerevisiae BSP 1 were fused. The resulting respiration-competent hybrid with K3 type killer activity was selected on media containing a non-fermentable carbon source and by a killer zone assay in a plate test, respectively. The fusant was similar to the parent strain in its fermentation and sugar utilization patterns, growth rate, dough-raising properties and osmotolerance. The newly constructed S. cerevisiae BSP 1 (K3) inhibited the growth of C tropicalis in a pH range from 3.5 to 5.0 and over a temperature range of 20-30degreesC.

Subject Keywords

Food Science, Microbiology, General Medicine

URI

https://hdl.handle.net/11511/67248

Journal

FOOD MICROBIOLOGY

DOI

https://doi.org/10.1016/j.fm.2004.03.012

Collections

Department of Biology, Article

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Citation Formats

F. Izgu, D. Altinbay, and Y. Derinel, “Immunization of the industrial fermentation starter culture strain of Saccharomyces cerevisiae to a contaminating killer toxin-producing Candida tropicalis,” FOOD MICROBIOLOGY, pp. 635–640, 2004, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/67248.