Paper Based Glucose Biosensor Using Graphene Modified with a Conducting Polymer and Gold Nanoparticles

Date

2017-01-01

Author

GOKOGLAN, Tugba Ceren
KESİK, Melis
SOYLEMEZ, Saniye
YÜKSEL, Recep
Ünalan, Hüsnü Emrah
Toppare, Levent Kamil

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In this work a novel approach was reported for glucose detection using disposable paper based electrodes consisting graphene modified with a conducting polymer, glucose oxidase (GOx) and gold nanoparticles. For the first time, a conducting polymer; poly(9,9-di-(2-ethylhexyl)-fluorenyl-2,7-diyl)-end capped with 2,5-diphenyl-1,2,4-oxadiazole (PFLO) was spread over graphene coated papers and used as a matrix for glucose detection. To finalize the desired biosensor, the enzyme GOx was immobilized with gold nanoparticles (AuNPs) via physical adsorption technique. Oxygen consumption was followed at -0.7 V vs Ag wire in a 50 mM, pH 6.0 phosphate buffer. The amperometric response of the GOx/graphene/PFLO/AuNPs-modified electrode was found to be linearly proportional to the glucose concentration. The fabricated biosensor revealed a high sensitivity for glucose in a linear range from 0.1 to 1.5 mM with a low detection limit of 0.081 mM at a signal-to-noise ratio of 3. K-M(App), I-max, and sensitivity were calculated as 0.229 mM, 2.198 mu A, and 7.357 mu A/mM cm(2), respectively. Finally, the constructed disposable biosensor was successfully utilized for the determination of glucose in commercial beverages. (C) 2017 The Electrochemical Society. All rights reserved.

Subject Keywords

Renewable Energy, Sustainability and the Environment, Electrochemistry, Materials Chemistry, Electronic, Optical and Magnetic Materials, Surfaces, Coatings and Films, Condensed Matter Physics

URI

https://hdl.handle.net/11511/47114

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Department of Metallurgical and Materials Engineering, Article