Applications

Download

index.pdf

Date

2014-01-01

Author

Gurel, Levent
Ergül, Özgür Salih

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

152
views

0
downloads

Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP). ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP). Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol dm-3 , denoted as A, B, C, D and E, respectively) were compared. Mineral formation was confirmed by IR and Raman, SEM, ICP-OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water. Ca was incorporated into mineral to a greater extent than Mg in samples mineralized in media A-D. Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA). Mineral formed in medium C was a combination of CDHA and an amorphous phase. Mineral formed in medium D was an amorphous phase. Mineral formed in medium E was a combination of crystalline and amorphous MgP. Young’s modulus and storage modulus decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E. Attachment and vitality of osteoblastic MC3T3- E1 cells were higher on samples mineralized in media B-E, containing Mg, than in those mineralized in medium A, not containing Mg. Hence, mineral composition, mechanical and biological properties of enzymatically mineralized hydrogels can be adjusted by varying the mineralization medium.

URI

https://hdl.handle.net/11511/47169

Journal

MULTILEVEL FAST MULTIPOLE ALGORITHM (MLFMA) FOR SOLVING LARGE-SCALE COMPUTATIONAL ELECTROMAGNETICS PROBLEMS

DOI

https://doi.org/10.1002/term.1875

Collections

Department of Electrical and Electronics Engineering, Article

Suggestions

OpenMETU
Core

Development of enhanced ultrafiltration methodologies for the resolution of racemic benzoin Olceroglu, Ayse Hande; Çalık, Pınar; Yılmaz, Levent (Elsevier BV, 2008-09-15) In the scope of achieving the separation of chiral molecules, enzyme enhanced ultrafiltration (EEUF), a new method based on polymer enhanced ultrafiltration (PEUF), utilizing apoenzymes as ligands, was developed. Benzoin was chosen as the model chiral molecule. Bovine serum albumin (BSA) and apo form of benzaldehyde lyase (BAL) (E.C. 4.1.2.38) were used as chiral ligands in PEUF and EEUF experiments, respectively. In order to bind to the target enantiomer well, the addition of ligand to the benzoin solution...
Addressing challenges in the ultrafiltration of biomolecules from complex aqueous environments Büküşoğlu, Emre; Koku, Harun; Çulfaz Emecen, Pınar Zeynep (Elsevier BV, 2020-04-01) Substantial progress has been made in ultrafiltration processes toward the separation of colloidal species from aqueous environments. Studies, especially in the past decade, have advanced the separations using functional and nano-structured membrane materials and a thorough understanding of colloid-membrane and colloid-colloid interactions in aqueous media. In this context, this review describes the recent advances in ultrafiltration of biomolecular species, most specifically proteins, from complex environm...
A theoretical study on coenzyme Q(10) and its reduced form. AM1 treatment Türker, Burhan Lemi (2000-01-01) Coenzyme Q(10) (CoQ(10)) and its reduced form, ubiquinol (CoQ(10)H(2)) were subjected to semiempirical quantum chemical treatment at the level of AM1-RHF type calculations. Both of the structures are stable having negative total and binding energies and exothermic heats of formation. The frontier molecular orbital characteristics, electrostatic contour diagrams and charge distributions of these structures were presented.
Production of flexible polymeric photoanodes using binder-free electrophoretic deposition and compression for dye-sensitized solar cells Kocaoglu, Bahadir Can; Özenbaş, Ahmet Macit (2015-04-29) Electrophoretic deposition (EPD) of titania nanoparticles have been utilized in order to form the nanoporous photoanodes of dye-sensitized solar cells (DSSCs) on flexible polymeric (PEN/PET) substrates. Highly uniform, adherent nanoporous thick films were produced by uniformly dispersing the titania nanoparticles in alcoholic suspensions without any binders. Suspension performances of each type of isopropanol based mixtures have been examined. Additionally, the effect of acetylacetone addition in suspension...
Background Orta Doğu Teknik Üniversitesi , Enformatik Enstitüsü (Orta Doğu Teknik Üniversitesi Enformatik Enstitüsü; 2022-10) Glucose-6-phosphate dehydrogenase (G6PD, E.C. 1.1.1.49) is a ubiquitous enzyme that has a housekeeping role in all cells. This enzyme catalyzes the first step in the pentose phosphate pathway (PPP) converting glucose 6-phosphate (G6P) to produce 6- phosphogluconolactone with concomitant reduction of oxidized nicotinamide adenine dinucleotide phosphate (NADP+) to reduced nicotinamide adenine dinucleotide phosphate (NADPH). NADPH plays a key role in protecting cells against oxidative stress by regulating the ...

Citation Formats

L. Gurel and Ö. S. Ergül, “Applications,” MULTILEVEL FAST MULTIPOLE ALGORITHM (MLFMA) FOR SOLVING LARGE-SCALE COMPUTATIONAL ELECTROMAGNETICS PROBLEMS, pp. 369–410, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47169.