PRODUCTION OF THE LEPIDOPTERA-SPECIFIC CRYSTAL PROTEIN BY A LOCAL ISOLATE OF BACILLUS-THURINGIENSIS

Date

1993-12-01

Author

AFKHAMI, P
Özcengiz, Gülay
ALAEDDINOGLU, NG

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A local isolate of Bacillus thuringiensis (B.t.81) produced crystal protein was identified as a cry I gene of Class I. The synthesis and assembly of crystal complements were investigated at intervals throughout the growth cycle. Incubation temperature had a marked effect on toxin synthesis; production being the highest at 25-degrees-C and the lowest at 42-degrees-C. The mutants of B. t. 81, unable to synthesize crystal protein complements, have also been described.

Subject Keywords

Biotechnology, General Medicine

URI

https://hdl.handle.net/11511/47895

Journal

BIOTECHNOLOGY LETTERS

DOI

https://doi.org/10.1007/bf00130307

Collections

Department of Biology, Article

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PLASMID PATTERNS OF BACILLUS-THURINGIENSIS 81 AND ITS CRYSTAL-NEGATIVE MUTANTS AFKHAMI, P; Özcengiz, Gülay; ALAEDDINOGLU, NG (Springer Science and Business Media LLC, 1993-12-01) A local isolate of Bacillus thuringiensis (B.t. 81) contained five distinct plasmids Relative amounts of these plasmids varied at different stages of growth.Two Cry- mutants of the strain maintained all plasmids. The mutants, however, exhibited some variations in the patterns of large plasmids depending on the composition of culture medium even though they failed to produce crystal protein. Restriction analysis of the plasmids was also carried out for future cloning studies.
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Citation Formats

P. AFKHAMI, G. Özcengiz, and N. ALAEDDINOGLU, “PRODUCTION OF THE LEPIDOPTERA-SPECIFIC CRYSTAL PROTEIN BY A LOCAL ISOLATE OF BACILLUS-THURINGIENSIS,” BIOTECHNOLOGY LETTERS, pp. 1253–1258, 1993, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47895.