Production of neutral and alkaline extracellular proteases by the thermophilic fungus, Scytalidium thermophilum, grown on microcrystalline cellulose

Date

2002-07-01

Author

Ifrij, IH
Ögel, Zümrüt Begüm

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

194
views

0
downloads

Extracellular proteases produced by Scytalidium thermophilum, grown on microcrystalline cellulose, were most active at pH 6.5-8 and 37-45 degreesC when incubated for 60 min. Highest protease activity was at day 3 where endoglucanase activity was low. Protease activity measurements with and without the protease inhibitors, p-chloromercuribenzoate, PMSF, antipain, E-64, EDTA and pepstatin A, suggest production of thiol-containing serine protease and serine proteases. Endoglucanase and Avicel-adsorbable endoglucanase activity in culture medium was not significantly affected by protease inhibitors.

Subject Keywords

Biotechnology, General Medicine

URI

https://hdl.handle.net/11511/57873

Journal

BIOTECHNOLOGY LETTERS

DOI

https://doi.org/10.1023/a:1016026904687

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

PRODUCTION OF THE LEPIDOPTERA-SPECIFIC CRYSTAL PROTEIN BY A LOCAL ISOLATE OF BACILLUS-THURINGIENSIS AFKHAMI, P; Özcengiz, Gülay; ALAEDDINOGLU, NG (Springer Science and Business Media LLC, 1993-12-01) A local isolate of Bacillus thuringiensis (B.t.81) produced crystal protein was identified as a cry I gene of Class I. The synthesis and assembly of crystal complements were investigated at intervals throughout the growth cycle. Incubation temperature had a marked effect on toxin synthesis; production being the highest at 25-degrees-C and the lowest at 42-degrees-C. The mutants of B. t. 81, unable to synthesize crystal protein complements, have also been described.
SUBCELLULAR-DISTRIBUTION OF ACCUMULATED HEAVY-METALS IN SACCHAROMYCES-CEREVISIAE AND KLUYVEROMYCES-MARXIANUS YAZGAN, A; Özcengiz, Gülay (Springer Science and Business Media LLC, 1994-08-01) Kluyveromyces spp. have been found to be more efficient than a CUP1(R) strain of S. cerevisiae in heavy metal resistance and accumulation. The present study describes the subcellular distribution of the accumulated metals (Ae, Cd, Cu) in S. cerevisiae and K. marxianus. Absorption by insoluble cellular material of the metals appears as the main mechanism of metal accumulation in both organisms.
Determination and metabolic engineering of rate limiting reactions in aromatic amino acid pathway in Bacillus subtilis for L-phenylaianine production Guzide, Calik; Yasemin, Demirci; Pinar, Calik; Ozdamar, Tuncer H. (2007-09-01) Rate limiting reactions in the aromatic-group amino acid pathway (AAAP) in Bacillus subtilis for l-phenylalanine (Phe) production were determined as reported elsewhere (Özçelik-Şenver et al., 2004). The biochemical reactions in the AAAP start with the reaction using phosphoenolpyruvate (PEP) and erythrose 4-phosphate (E4P) synthesised in the glycolysis pathway and the PPP, respectively, leading to the formation of Phe via chorismate by branching at prephenate. The branch-point metabolites E4P supplied in vi...
Kinetic analyses of the effects of temperature and light intensity on growth, hydrogenm production and organic acid utilization by rhodobacter capsulatus Sevinç, Pelin; Gündüz, Ufuk; Department of Biotechnology (2010) Effects of temperature and light intensity on photofermentative hydrogen production by Rhodobacter capsulatus DSM1710 by use of acetic and lactic acids as substrates were studied. Experiments were conducted at 20, 30 and 38oC incubator temperatures under light intensities in the 1500 – 7000 lux range. pH of the medium and quantity of hydrogen forming together with quantity of biomass, and concentrations of acetic, lactic, formic, butyric and propionic acids in the medium were determined periodically. Growth...
Regulatory effects of oxygen transfer on overexpression of recombinant benzaldehyde lyase production by Escherichia coli BL21 (DE3) Kaya-Çeliker, Hande; Angardi, Vahideh; Çalık, Pınar (Wiley, 2009-10-27) Effects of oxygen transfer (OT) on benzaldehyde lyase (BAL) production by recombinant E. coli BL21 (DE3) pLySs were investigated at an air‐inlet rate of QO/VR = 0.5 vvm and agitation rates of N = 250, 500, 625, and 750/min; and at constant dissolved oxygen (DO) concentrations of CDO = 0.04 and 0.08 mol/m3, on a glucose based defined medium. The highest cell concentration (CX = 3.0 kg/m3) and volumetric BAL activity (A = 1095 U/cm3) were obtained at CDO = 0.08 mol/m3. KLa increased with agitation rate and de...

Citation Formats

I. Ifrij and Z. B. Ögel, “Production of neutral and alkaline extracellular proteases by the thermophilic fungus, Scytalidium thermophilum, grown on microcrystalline cellulose,” BIOTECHNOLOGY LETTERS, pp. 1107–1110, 2002, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57873.