Production of neutral and alkaline extracellular proteases by the thermophilic fungus, Scytalidium thermophilum, grown on microcrystalline cellulose

Date

2002-07-01

Author

Ifrij, IH
Ögel, Zümrüt Begüm

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Extracellular proteases produced by Scytalidium thermophilum, grown on microcrystalline cellulose, were most active at pH 6.5-8 and 37-45 degreesC when incubated for 60 min. Highest protease activity was at day 3 where endoglucanase activity was low. Protease activity measurements with and without the protease inhibitors, p-chloromercuribenzoate, PMSF, antipain, E-64, EDTA and pepstatin A, suggest production of thiol-containing serine protease and serine proteases. Endoglucanase and Avicel-adsorbable endoglucanase activity in culture medium was not significantly affected by protease inhibitors.

Subject Keywords

Biotechnology, General Medicine

URI

https://hdl.handle.net/11511/57873

Journal

BIOTECHNOLOGY LETTERS

DOI

https://doi.org/10.1023/a:1016026904687

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Graduate School of Natural and Applied Sciences, Article

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Citation Formats

I. Ifrij and Z. B. Ögel, “Production of neutral and alkaline extracellular proteases by the thermophilic fungus, Scytalidium thermophilum, grown on microcrystalline cellulose,” BIOTECHNOLOGY LETTERS, pp. 1107–1110, 2002, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57873.